pLVX-TRE3G

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-TRE3G , pLVX TRE3G
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: TRE3GV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7819 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: 嘌呤霉素(Puromycin)
备注: 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体。
产品目录号: 631191
稳定性: /
组成型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-TRE3G载体图谱



pLVX-TRE3G多克隆位点


pLVX-TRE3G载体特征

载体简介

载体序列

LOCUS       pLVX-TRE3G   7819 bp    DNA   circular            31-MAY-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.631191, 631193,631187, 631189  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|         
FEATURES             Location/Qualifiers
     misc_feature    1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT/CTS
     misc_feature    2600..3103
                     /label=pPGK
     misc_feature    3124..3723
                     /label=Puro(R)
     misc_feature    3741..4332
                     /label=WPRE
     3'clip          4536..5172
                     /label=3'\LTR
     misc_feature    complement(5642..6312)
                     /label=pUC\ori
     misc_feature    2556..2599
                     /label=MCS
     misc_feature    2210..2555
                     /label=pTRE3G
     misc_feature    complement(6457..7317)
                     /label=Amp(R)
BASE COUNT     1973 a      1955 c      2040 g      1851 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc cgggcccgcg gccgccccgg 
     2581 gacgcgtcat atggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 
     2641 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 
     2701 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 
     2761 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 
     2821 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 
     2881 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 
     2941 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 
     3001 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 
     3061 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 
     3121 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 
     3181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 
     3241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 
     3301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 
     3361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 
     3421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 
     3481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 
     3541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 
     3601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 
     3661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 
     3721 tgacggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg 
     3781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     3841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     3901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     3961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     4021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     4081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 
     4141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     4201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     4261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     4321 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag 
     4381 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga 
     4441 ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 
     4501 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac 
     4561 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc 
     4621 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc 
     4681 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt 
     4741 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt 
     4801 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga 
     4861 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg 
     4921 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt 
     4981 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 
     5041 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 
     5101 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 
     5161 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 
     5221 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta 
     5281 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     5341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     5401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     5461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     5521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     5581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     5641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     5701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     5761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     5821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     5881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     5941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     6001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     6061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     6121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     6181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt 
     6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     6361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     6421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     6481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     6541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     6601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     6661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     6721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 
     6781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     6841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     6901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     6961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     7021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 
     7081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     7141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     7201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     7261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     7321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     7381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     7441 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca 
     7501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     7561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag 
     7621 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag 
     7681 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 
     7741 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 
     7801 gtactacaaa cttagtagt 
//

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