| 质粒名称: | pAAVLINK-EF1a-5'-COL5A1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001668 |
| 产品用途: | 5' AAVLINK plasmid for COL5A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL5A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-COL5A1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-COL5A1载体质粒基本信息
pAAVLINK-EF1a-5'-COL5A1质粒图谱载体图谱和pAAVLINK-EF1a-5'-COL5A1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-COL5A1质粒载体简介
pAAVLINK-EF1a-5'-COL5A1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-COL5A1 6983 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6983)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6983
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..4123
/codon_start=1
/label=COL5A1
/translation="MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKV
LDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILT
TVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDG
KWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQ
QLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYE
DPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDY
VPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADD
LEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEP
AIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPG
TMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPP
GSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGL
AGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPG
PPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLP
GMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGE
DGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPG
LPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKP
GPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGK
TGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPG
KDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRP
GPQGPPGPAGEKGAPGEKGPQGPAGRDGLQ"
misc_feature 1353..1358
/label=SDS
misc_feature 3034..3039
/label=SDS
misc_feature 3542..3547
/label=SDS
intron 4124..4164
/label=Splicing Donor
misc_feature 4168..4201
/label=lox2272-JT1571
repeat_region 4246..4386
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4246..4375
/label=AAV2 ITR
rep_origin 4461..4916
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5198..5302
/gene="bla"
/label=AmpR promoter
CDS 5303..6163
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6334..6922
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggacgt
721 ccatacccgc tggaaagcgc gcagcgcgct ccgcccgggc gccccgctgc tgcccccgct
781 gctgctgctg ctgctgtggg cgccgcctcc gagccgcgca gctcagccag cagatctcct
841 gaaggttcta gattttcaca acttgcctga tggaataaca aagacaacag gcttttgcgc
901 cacgcggcga tcttccaaag gcccggatgt cgcttacaga gtcaccaaag acgcgcagct
961 cagcgcaccc accaagcagc tgtaccctgc gtctgcattt cccgaggact tctccatcct
1021 aacaactgtg aaagccaaga aaggcagcca ggccttcctg gtctccatct acaacgagca
1081 gggtatccag cagattgggc tggagctggg ccgctctccc gtcttcctct acgaggacca
1141 cacggggaag cctggcccgg aagactaccc cctcttccgg ggcatcaacc tgtcagatgg
1201 caagtggcac agaattgctc tcagcgtcca caagaaaaat gtcaccttga tcctcgactg
1261 taaaaagaag accaccaaat tcctcgaccg cagcgaccac cccatgatcg acatcaatgg
1321 catcatcgtg tttggcaccc ggatcctgga tgaggaggtg tttgagggtg acatccagca
1381 gctgctcttt gtctcggacc accgggcagc ttatgattac tgtgagcact acagccctga
1441 ctgtgacacc gcagtacctg acaccccaca gtcgcaggac cccaatccag atgaatatta
1501 cacggaagga gacggcgagg gtgagaccta ttactacgaa tacccctact acgaagaccc
1561 cgaagaccta gggaaggagc ccacccccag caagaagccc gtggaagctg ccaaagaaac
1621 cacagaggtc cccgaggagc tgaccccgac ccccacggaa gctgctccca tgcctgaaac
1681 cagtgaaggg gctgggaagg aagaggacgt cggcatcggg gactatgact acgtgcccag
1741 tgaggactac tacacgccct caccgtatga tgacctcacc tatggcgagg gggaggagaa
1801 ccccgaccag cccacagacc caggcgctgg ggccgaaatt cccaccagca ccgccgacac
1861 ctccaactcc tccaatccag ctccgcctcc aggggaaggt gcggatgact tggaggggga
1921 gttcactgag gaaacgatcc ggaaccttga cgagaactac tacgacccct actacgaccc
1981 caccagctcc ccgtcggaga tcgggccggg aatgccggcg aaccaggata ccatctatga
2041 agggattgga ggacctcggg gcgagaaagg ccaaaaggga gaaccagcga ttatcgagcc
2101 gggcatgctc atcgagggcc cgcctggccc agaaggcccc gcgggtcttc ccggacctcc
2161 aggaaccatg ggtcccactg gccaagtcgg ggaccctgga gaaaggggcc cccctggacg
2221 cccaggcctt cctggggccg atggcctgcc cggtcctcca ggaaccatgc tcatgctgcc
2281 cttccggttt ggaggtggcg gcgatgcggg ctccaaaggc cccatggtct cagcccagga
2341 gtcccaggcg caagccattc tccagcaggc caggttggca ctgaggggac cagctggccc
2401 gatgggtctc acagggagac ctggccctgt gggtccccct gggagcggag gtttgaaggg
2461 cgagccggga gacgtggggc ctcagggtcc tcgaggtgtg caaggcccgc ctggtccggc
2521 cgggaagccc ggaagacggg gtcgggctgg gagtgatgga gccagaggaa tgcctggaca
2581 aactggcccc aagggtgacc ggggtttcga cggcctggct gggttgccag gcgagaaggg
2641 ccacaggggt gaccctggtc cttccggccc accaggacct ccgggagacg atggagaaag
2701 gggtgacgac ggagaagttg ggcccagggg gctgcctggg gagcccgggc cacgtggtct
2761 gcttgggccg aaggggcccc caggtcctcc cggacctccc ggtgtcacgg gtatggacgg
2821 ccagccgggg ccaaaaggaa atgtgggtcc ccagggagag cctggccccc caggacagca
2881 gggtaatcca ggcgcccagg gtcttccagg cccccagggt gcaattggtc ctccaggaga
2941 aaagggtccc ttggggaaac caggccttcc aggaatgccc ggtgctgacg gacccccggg
3001 acaccctggc aaagaaggcc ctccaggaga gaaaggaggt cagggtccac ctggccccca
3061 gggtccgatt ggctacccag gtcctcgagg agtcaagggg gccgatggca tccgtggtct
3121 gaagggcaca aagggcgaga agggtgaaga cggctttcct gggtttaaag gagacatggg
3181 catcaagggt gatcgggggg agatcggccc acccggtccc aggggagaag atggccctga
3241 aggcccaaag ggtcgcggag gtcccaatgg tgaccccggt cctctgggac cccctgggga
3301 gaagggaaaa ctcggagtcc cagggttacc agggtatcca ggaagacaag gaccaaaggg
3361 ctctattgga ttccctggat ttcctggcgc caatggagag aagggcggca gggggacccc
3421 tggaaagcca ggaccgcggg ggcagcgagg cccaacgggt ccgaggggtg aaagaggccc
3481 ccggggcatc actgggaagc ctggccccaa gggcaactcc ggaggtgacg gcccagctgg
3541 ccctcctggt gaacggggac ccaatggacc ccaaggaccc acaggatttc ctggaccaaa
3601 gggcccccct ggccctccag gcaaggatgg actcccagga caccctggac agagaggcga
3661 gactggtttc caaggcaaga ccggccctcc aggccccccc ggcgtggtcg gccctcaggg
3721 tcccacggga gaaacgggcc caatgggtga gcgtggccac cctgggcccc ctggaccccc
3781 cggtgaacag gggcttccgg gccttgctgg aaaagaaggg acgaagggtg acccaggccc
3841 tgcaggcctc cctgggaaag atggccctcc aggattacgt ggtttccctg gggaccgagg
3901 gcttcctggt ccagtgggag ctcttggact gaaaggcaat gaagggcccc ctggcccacc
3961 aggccctgcg ggatctccag gggagagagg tccagctgga gccgctgggc ccatcggaat
4021 tccagggaga cctgggcccc agggaccccc agggccggca ggagagaaag gggctcctgg
4081 cgagaaaggc ccacaaggcc cagctggccg agacggtctc caggtaagta tcaaggttac
4141 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
4201 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
4261 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
4321 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
4381 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
4441 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4501 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
4561 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4621 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
4681 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
4741 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
4801 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
4861 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
4921 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
4981 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
5041 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
5101 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
5161 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
5221 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
5281 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
5341 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
5401 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
5461 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
5521 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
5581 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
5641 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
5701 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
5761 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
5821 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
5881 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
5941 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
6001 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
6061 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
6121 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
6181 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
6241 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
6301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
6361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
6661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
6721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
6781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
6841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
6901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
6961 ttgctggcct tttgctcaca tgt
//
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pAAVLINK-EF1a-5'-COL5A1质粒载体应用举例
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