| 质粒名称: | P362-FBP_12 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 139703 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_220d |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs |
| 物种来源: | Neonothopanus nambi and Aspergillus nidulans |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | various |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AarI（克隆时销毁） |
| 3' 克隆位点: | AarI（克隆时销毁） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | P362-FBP_12 was a gift from Daniel Voytas (Addgene plasmid # 139703 ; http://n2t.net/addgene:139703 ; RRID:Addgene_139703) |
| 参考文献部分: | Building customizable auto-luminescent luciferase-based reporters in plants. Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF. Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. 10.7554/eLife.52786 PubMed 32209230 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS P362-FBP_12 22699 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION T-DNA encoding Fungal Bioluminescent Pathway Components, complete
pathway.
ACCESSION .
VERSION .
KEYWORDS P362-FBP_12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 22699)
AUTHORS Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
R, Rizvi F, Imaizumi T, Voytas DF
TITLE Building customizable auto-luminescent luciferase-based reporters in
plants.
JOURNAL Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
PUBMED 32209230
REFERENCE 2 (bases 1 to 22699)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..22699
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(5..22)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5..21)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(14..36)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 224..248
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 1549..2178
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2607..3680
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3746..3940
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(4176..4198)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 4284..4424
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4339..4358
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4439..4456)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4610..5198)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4690..4709)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5285..6079)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6504..6528
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6606..6780
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6837..7634)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6948..6967)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 7558..7577
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(7697..8373)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 8564..8585
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8600..8630
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8638..8654
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8643..8665
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8662..8678
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8662..8678
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 10808..11153
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 12304..12504
/label=gene 7 terminator
/note="transcription terminator for octopine-type Ti
plasmid gene 7"
promoter 12541..12921
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
terminator 14193..14445
/label=MAS terminator
/note="mannopine synthase terminator"
promoter 14557..14902
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 14921..14976
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
polyA_signal 20109..20283
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
promoter 20804..21149
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 21971..22678
/label=OCS terminator
/note="octopine synthase terminator"
ORIGIN
1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
8701 tacccgggga tcggcgcgcc ctatggagaa tatcattttt aagtaaaatt ttgaattcaa
8761 atgttacgtg tattatttaa ttcatcaatt tgccttgtca tagcgagtac attacaaaca
8821 tcacatatat ttgattgatt gtcaaaaaat atcaaaatat atatcaattt taagaggtat
8881 aggtgtctaa tatgtactag ccctaattta aatatctaaa ttaattattc ggatgaatct
8941 atataccatc tttttaatgg acacccaaaa tcacacatca aacatcatat acatgttgaa
9001 aacatattat tgatatagct acatatatgt tttaatataa ataaaagacg agtcatatat
9061 tcaaaaatta agaatcaaat aattttaatt tatttaatat tcaaaactta atactattta
9121 aatttagata ttctaatttt aatacacgtc tgataaaata gatgaggact aaataaataa
9181 tttgagacta tcttttcttt atttggcggc ccacaaataa tttagattct cgtaaccccc
9241 tctttttctc tcactgaaaa agcacaatcc gtgtccaaac acaaagaagc actcgacacc
9301 gtagatctcc attcagatca acggcttata ttcagttttc tccattcacg tggatcgaca
9361 ttcttatccg tccgattatc aataaatttc ccaaaattta gcggccatga ttttaacccc
9421 gcctcatttc aaaccgccca cgaaatcctc gacgcccaaa ttcaccaact ataaatagcc
9481 accaccatcc ccttcatcaa tcatcaaatt tcataaccct agaatcatca cctttttcaa
9541 atttcaatgg ctcctatctc ctccacctgg tccagattaa tcaggttcgt agctatagaa
9601 acgtcactcg tgcatatagg cgaaccgatt gatgcaacta tggacgtagg gcttgctcgt
9661 cgagaaggga aaactattca ggcctatgag attatcggat ctggcagcgc cctggacctt
9721 tctgcacagg tgagtaagaa cgtgctcaca gtacgagaat tgttgatgcc actttctaga
9781 gaagagataa agacggtcag atgcttaggc cttaactatc ctgtccacgc aacagaagcc
9841 aatgttgctg taccgaagtt tccgaacctg ttctacaaac cagtcacaag tctcatcggg
9901 cctgatggtc ttatcacgat ccctagtgta gtgcaaccgc ctaaggaaca tcagtcagat
9961 tatgaagctg aacttgtgat tgtaatcgga aaggccgcca agaacgtgtc tgaggacgaa
10021 gccttggatt atgtcttagg ctatacagct gctaacgata ttagtttccg aaaacaccag
10081 ctcgcagtct cacagtggtc attctcaaaa ggatttggat ctctccttct cactatacgt
10141 atggcccaga cacatagtgg aaacataaat aggttctcac gtgatcaaat ctttaatgtt
10201 aagaaaacaa tcagcttttt gtctcaagga actaccctcg aacctgggtc tatcattttg
10261 actggcaccc ctgatggggt cggcttcgtc aggaacccgc ccttatactt aaaagatggg
10321 gatgaagtga tgacttggat aggttccggc attggtacgc ttgctaacac cgttcaagaa
10381 gagaaaacat gctttgcaag cggtgggcat gagtaggctt ttatgttggt gatatggtgg
10441 taaatgtagg gatttagttt acaattgcgt atgtctgtgt tggatatctg tagtgctgtt
10501 cttatggctt agatcttgta atttctcatt acagtatcaa tgaatagata tcagtttcta
10561 gtgatgacat tggttcgtct tttagctgtt gattaatttt tcttaattga ttcatcctat
10621 tgcaattctt ctgaatttaa attgtatact gtgaaattaa gaaaattctt gaaattaatg
10681 agaatttgag taatagcgct gttagagctc tgacggcgcg ccctatggag gtcaacatgg
10741 tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa
10801 gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc
10861 cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc
10921 atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag
10981 atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa
11041 agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc
11101 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctcga
11161 gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
11221 ttacatttac aattatcgat acaatggtgc aggacacttc aagtgctagc acaagtccaa
11281 ttttaaccag atggtatata gacactcgac ctctcacggc aagtaccgcc gcacttccgc
11341 tgctggaaac gctgcaacca gctgatcaga tctccgtcca aaaatattat catctcaagg
11401 acaagcacat gtcacttgcc tccaaccttt tgaagtattt attcgttcat cgaaattgcc
11461 gaataccatg gagtagtatt gtcatatcaa gaacgcccga cccgcacaga aggccttgct
11521 atatacctcc ttcaggttca caagaggact ccttcaaaga cggatatacg ggaatcaacg
11581 tagaattcaa cgttagtcac caagccagca tggtggcaat cgcaggtacg gctttcacac
11641 ctaatagcgg tggagatagc aaactcaaac cggaggtcgg aatagatata acatgtgtaa
11701 atgagaggca gggcaggaat ggagaagaaa gatccttgga atccctgagg cagtatatag
11761 atatattcag cgaagtattc agcaccgcag agatggctaa tataaggcga cttgatgggg
11821 tttcctcaag ttcccttagt gcagacagac tcgtagatta tggataccga ttattctaca
11881 cctattgggc cttaaaagag gcttatatca agatgacagg tgaggcctta ttggctcctt
11941 ggttgagaga gctggagttt tccaacgtgg tcgctccagc cgctgtcgcc gaaagcgggg
12001 actctgctgg agactttggc gaaccgtaca ctggcgtgcg tacaacattg tataagaacc
12061 tcgtagagga cgtacgaatt gaggtagctg ctttgggagg agattatctg ttcgcaacag
12121 ccgctagagg tggtggtata ggcgcatcct cacgtcctgg tggtggccca gatggctctg
12181 gaattagatc ccaggaccct tggaggccgt tcaagaaact cgatatagag cgtgacatac
12241 agccatgtgc tacgggcgtt tgtaattgcc tgtcctcagg ataggcttga tcccccgtcg
12301 acagctagct atatcatcaa tttatgtatt acacataata tcgcactcag tctttcatct
12361 acggcaatgt accagctgat ataatcagtt attgaaatat ttctgaattt aaacttgcat
12421 caataaattt atgtttttgc ttggactata atacctgact tgttatttta tcaataaata
12481 tttaaactat atttctttca agatcgctgt tagagctcgg acggcgcgcc ctatggagat
12541 ttttcaaatc agtgcgcaag acgtgacgta agtatccgag tcagttttta tttttctact
12601 aatttggtcg tttatttcgg cgtgtaggac atggcaaccg ggcctgaatt tcgcgggtat
12661 tctgtttcta ttccaacttt ttcttgatcc gcagccatta acgacttttg aatagatacg
12721 ctgacacgcc aagcctcgct agtcaaaagt gtaccaaaca acgctttaca gcaagaacgg
12781 aatgcgcgtg acgctcgcgg tgacgccatt tcgccttttc agaaatggat aaatagcctt
12841 gcttcctatt atatcttccc aaattaccaa tacattacac tagcatctga atttcataac
12901 caatctcgat acaccaaatc gaatggcatc ctttgaaaat tcactcagtg tcttaatagt
12961 tggggctggc ttaggtggtt tggccgctgc tatagcactg cgaaggcaag gacatgtagt
13021 caagatatac gatagttctt cattcaaggc cgaattggga gcaggattag ctgtaccacc
13081 caatactctc cgttccctgc aacaactggg ctgtaataca gaaaacctga acggagtaga
13141 caacctctgc tttacggcca tgggatacga cgggagtgta ggtatgatga ataatatgac
13201 cgactaccgt gaggcatacg gcacttcctg gataatggta catcgagtgg atctccacaa
13261 cgagctgatg cgagtcgcct tggatccggg cgggttaggg cctcctgcca ctcttcacct
13321 taaccatcgt gttacgttct gcgatgtgga tgcctgtaca gtcacgttta cgaacgggac
13381 gacacagtct gctgacttga ttgtcggagc tgatggtatt aggtctacga ttcgaagatt
13441 cgttctggag gaggacgtga ctgttccagc tagcggaatt gtcggctttc gatggttggt
13501 ccaggccgat gctctcgacc catatcccga actggattgg attgtcaaaa aaccaccctt
13561 gggagccagg ttgatttcta ctccacagaa cccgcagagt ggcgtaggac tggccgatag
13621 acgaacgatc attatttacg catgcagagg tgggaccatg gttaatgtat tagccgtaca
13681 cgacgacgaa agagatcaaa acactgctga ttggtccgtt cccgcatcaa aggatgattt
13741 attcagagtg tttcacgatt accatccaag attccgacga ctcttagagt tagcacagga
13801 cattaattta tggcagatga gggtggtccc ggtcttgaaa aaatgggtga acaagagggt
13861 atgcttgctg ggtgatgccg cccatgcttc actgcccaca cttggacaag gcttcgggat
13921 ggggttggag gatgcagtcg ctttgggcac gttgttgcct aagggaacga ccgcatcaca
13981 gatcgagaca aggctggccg tttacgaaca gctgcgaaag gatcgagctg agtttgtcgc
14041 cgcagagagc tatgaggagc aatatgtccc agagatgaga gggctgtacc ttaggtccaa
14101 agaattgaga gacagagtga tgggttatga tattaaggta gagtcagaga aggttttgga
14161 aacactgttg cgatcatcca actcagcttg agcttggact cccatgttgg caaaggcaac
14221 caaacaaaca atgaatgatc cgctcctgca tatggggcgg tttgagtatt tcaactgcca
14281 tttgggctga attgtagaca tgctcctgtc agaaattccg tgatcttact caatattcag
14341 taatctcggc caatatccta aatgtgcgtg gctttatctg tctttgtatt gtttcatcaa
14401 ttcatgtaac gtttgctttt cttatgaatt ttcaaataaa ttatccgctg ttagagctcc
14461 cggggcgcgc cctatggagg tcaacatggt ggagcacgac actctggtct actccaaaaa
14521 tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat
14581 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
14641 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
14701 agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
14761 aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgaca tctccactga
14821 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
14881 ttcatttcat ttggagagga cacgctactg tatttttaca acaattacca acaacaacaa
14941 acaacaaaca acattacaat tactatttac aattacaatg aatagtagta aaaaccctcc
15001 atccactctg ttagatgttt ttctggatac cgctaggaac cttgatacag catccagaaa
15061 tgtcctggag tgcggagaac acaggtggag ttacagagag ttggacacag tgtcatccgc
15121 attggcccaa caccttcgat acactgtggg tcttagcccc actgtagccg tgattagcga
15181 gaatcacccc tacatacttg cattaatgct cgccgtatgg aaactcggcg gaacgttcgc
15241 accgatagat gttcattccc cagccgaact tgttgctgga atgttgaata ttgtaagccc
15301 gtcatgtctt gtcattccta gctctgatgt gacaaatcag acattggcat gcgatcttaa
15361 tataccggtt gttgcattcc acccacatca aagtacaatt cccgaactca acaagaagta
15421 tctcacagat tctcagatca gtcctgacct gcccttcccc gaccctaatc gacctgcttt
15481 gtacttattc acgtcatctg caacgtctag gtccaatttg aagtgcgtgc cacttacaca
15541 cacattcata ttgcgaaact ccttaagtaa acgagcatgg tgcaaaagaa tgaggccaga
15601 gacagatttt gatggtatta gggtcctggg gtgggccccc tggagccatg tcctcgctca
15661 tatgcaggac attggccctc ttacattatt gaatgccggg tgctacgtat ttgccacgac
15721 cccatctacc tacccgacgg agctgaagga tgaccgtgat gttattagtt gcgccgcaaa
15781 tgccgtaatg tacaaaggtg ttaaatcctt cgcatgcctc ccattcgtgc ttgggggact
15841 caaggcttta tgtgagtcag agcccagtgt taaggcacaa ctgcaagtgg aggaacgagc
15901 ccagcttttg aagtcactcc aacacatgaa aatcctggaa tgcggggggg caatgctcga
15961 agtgagtgtg gcaagctggg ctatagagaa taggattcct atatctattg ggattggtat
16021 gacagagaca ggcggcgctc ttttcgctgg gccggtccaa gcaattcaaa ccggctttag
16081 cagtgaagat aaatttatcg aggacgcaac ctacttgctg gtcaaggacg attacgagag
16141 ccacgcagag gaggacataa acgagggcga gttagtggta aagtccagaa tgttacctag
16201 gggttatttg ggctataacg acccctcttt ctctgttgac gatgctggtt gggttacttt
16261 caaaactgga gatcgatatt cagtcactcc tgacggcaaa ttctcctggc ttggtcgaaa
16321 tactgatttc attcagatga cgagtggtga gactctcgat ccccgtccga ttgagtccct
16381 cctttgtgag tccagcctta tatcacgagc ctgtgtcatc ggggataagt tcctgaacgg
16441 accagccaca gctgtatgcg ctattataga actcgaacca acgactgttg agaaaggcca
16501 ggctcacagc agggatattg cccgaatctt tgcccccatc aatcgagatc ttcctccccc
16561 gttacgtata gcatggagtc acgtacttgt acttcagcca agcgagaaaa taccgatgac
16621 gaaaaagggt acaatcttcc gtaagaaaat cgaacaggtt ttcggttccg ctttaggagg
16681 cagtagcggc gacaactcac aagcaaccac tgacgcttca gtagttagga gggatgagtt
16741 gagcaacacg gtcaaacata tcataagcag agttttggga gtctccgacg atgaactcct
16801 ttggacactg agctttgctg agcttggcat gacgtccgct cttgctacta gaatagcaaa
16861 tgaattgaac gaagttctgg taggtgtgaa cctccccatc aatgcatgct acattcatgt
16921 ggacctgcct tctttgtcaa atgccgtgta tgcaaagtta gcacacctta aactcccgga
16981 taggacgccc gagccaagga aggccccagt tgaaaatccc ggtggaaagg aaatcgtaat
17041 agtaggtcag gcttttaggc ttccggggag tataaatgat gtcgcttctt tgagggacgc
17101 ttttttggct agacaagcat ccagtattat tactgaaata ccgccagata gatgggacca
17161 cgcatcattt tatccaaaag acatacgatt taataaagca gggctcgtcg acatcgctaa
17221 ctacgaccac agttttttcg gattgacagc cacggaggca ctgtacctgt caccaacgat
17281 gcgtttggca cttgaagtgt cctttgaagc cttggagaat gctaatatac ccgtgtccca
17341 gttaaagggc tctcaaacag ctgtatatgt cgcaaccacc gacgacgggt ttgagacact
17401 gctgaatgct gaggcaggat atgatgcata cacacgattc tatggcactg gtagggccgc
17461 ctctacggcc agtggacgaa ttagttattt actcgacgta cacggccctt caattaccgt
17521 agacactgct tgttcaggcg gtgctgtttg catagaccag gccatagact acctccaatc
17581 ctctagcgca gctgataccg ccataatctg cgccagcaac actcactgct ggccaggttc
17641 cttcatgttt ctgagtgctc aaggtatggt aagttccggg gggcgatgtg caacttttac
17701 gacggatgcc gacggctatg taccctctga aggtgctgtg gcatttatcc tcaaaacccg
17761 agaagcagct atgagagata aagatactat cttggcaact atcaaagcaa cgcagattag
17821 tcataatggt cgttcccaag gtttagtcgc accaaacgtc aattcacaag ctgatttaca
17881 cagaagctta ttgcagaaag ctggtttgag tccagccgac attcacttca tcgaagctca
17941 cgggacagga acgtcactcg gtgaccttag tgaaatacaa gcaattaatg atgcctacac
18001 gtcatctcag cctcgaacag ccggaccctt aatagtaagc gcaagtaaaa ccgtcattgg
18061 tcacacagag cctgctggac cgttggtagg catgctctca gttctcaact cctttaaaga
18121 gggcgcagtt cccggcttag ctcacctgac agcagataac ctcaatccgg ctttagactg
18181 ctcctccgtc cctttactta tcccctacca gcctgtccac ttggcagctc ctaagccaca
18241 cagggctgct gtgatgagtt atggcttctc cggcactctt ggaggtatag ttcttgaagc
18301 accggatgag gaaaggcttg aggaagaacc acccaacgat aagcctatgt tatttgttgt
18361 tagcgccaaa actcatactg cattgattga gtaccttgga cgataccttg aatttctgct
18421 ccaggccaat ccccaagact tctgcgatat ctgttacact agctgcgttg gacgtgagca
18481 ttacaggtac cgttttgcat gtgtggcaaa cgatatggaa gatttaattg gccaacttca
18541 gaagaggctt gggtctaagg ttccacctaa accgtcctat aaaagaggag ccctcgcttt
18601 tgctttcagt ggacaaggga cccagtttcg tggaatggca acagaattgg ctaaggcata
18661 tagtggtttt cgtaagatag tcagtgatct tgctaaaaga gcatcagaac tgtccggcca
18721 tgcaatcgac aggtttctgc ttgcatatga cattggggct gagaatgttg caccggatag
18781 tgaagctgat caaatctgca tattcgttta ccaatgtagt gttctcagat ggctccaaac
18841 aatggggatt cgtccgtcag ccgtgatcgg tcacagcctc ggtgagatta gcgcatccgt
18901 agctgctgga gcattgtcct tggactctgc ccttgacctc gtaataagta gagccaggtt
18961 actgaggtca tctacgaatg ccccagctgg catggcagct atgtctgcat cccaggatga
19021 agtagtcgaa ctcataggaa agctcgattt agataaagct aatagcctca gcgtgtccgt
19081 aataaacggc ccccagaaca ccgttgtttc aggatcttct gcagcaatag aaagtatcgt
19141 tgctctcgca aagggacgaa aaatcaaggc cagcgcactc aacattaacc aggcattcca
19201 tagtccttac gtcgattctg cagtacccgg cttacgagct tggagtgaga aacatatatc
19261 atcagctcga ccattgcaaa tccctttgta ctctaccctc ttgggcgctc aagtttctga
19321 gggtcaaatg ttaaatcccg atcactgggt agaccatgct aggaaacccg ttcagttcgc
19381 acaagcagcc actataatga aggaatcttt caccggagta ataatcgata ttggacctca
19441 agttgtagca tggtccctgc tcttaagcaa tggtcttact tccgtgaccg cactcgccgc
19501 aaagcgtggc cgttcacagc aagtggcctt cctctccgcc ttagctgatc tttatcagga
19561 ctacggcgtc gtaccagact ttgttggact gtacgcacaa caggaggacg cttccagact
19621 gaaaaagacc gatatcttaa cttacccctt tcagagggtt cgacgttacc cttcctttat
19681 accctctaga agggcaccca cccacgctca tgtccaagac gaggaaactc tgtcatcagg
19741 atcatcaact ccaaccttag aaaatactga cctggacagt gggaaggaat ctctgatggg
19801 accaacaaga gggcttttgc gagtcgatga tcttagggac tcaatagtca gttcagtcaa
19861 agatgtcctg gaattgaaga gtaacgaaga tctggacctc agcgagtccc ttaatgctct
19921 tggcatggac tctataatgt tcgctcaatt acgaaaaagg ataggggagg gcctcggcct
19981 gtccgttcca atggtttttc tcagcgatgc cttcagtatt ggagaaatgg tcagtaacct
20041 cgtggaacag gccgaagctt ccgaggacaa ttgagcttct ctagctagag tcgatcgaca
20101 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
20161 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
20221 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
20281 atcgctgtta gagctctccc ggcgcgccct atggacagat ttgccttttc aatttcagaa
20341 agaatgctaa cccacagatg gttagagagg cttacgcagc aggtatcatc aagacgatct
20401 acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca
20461 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
20521 gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga
20581 ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa
20641 tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac
20701 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact
20761 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
20821 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
20881 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
20941 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
21001 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
21061 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
21121 aaggaagttc atttcatttg gagagaacac gggggactaa tgcgtataaa tatctctctg
21181 tctagcttat tcgagagatt aagcaaactc agctctcgta gcatagctat tacatgtggt
21241 gtcgtcctcg cctctgctat tgcttttccc atcatacgac gagattatca gacgttctta
21301 gaggttggtc ctagctatgc ccctcaaaat tttcgaggct acattatcgt ttgcgtactc
21361 agcttgttcc gacaagagca aaaagggttg gcaatctacg acagactccc agagaagaga
21421 agatggctgg ccgatctccc atttcgtgaa ggtactagac ctagtataac atcacacata
21481 atacagagac agcgaactca acttgtagac caagagtttg ccacacgaga actgatagac
21541 aaagttattc ccagggtgca agcacgacat acagataaaa catttttgag cacgagcaag
21601 tttgaatttc atgctaaagc cattttcctg ttgccaagta ttcctataaa cgacccctta
21661 aacataccta gtcatgatac ggttcgtaga acgaaacgag agattgcaca catgcatgac
21721 taccacgact gtactcttca cttagctctt gcagctcaag atggaaaaga agtcctgaaa
21781 aaaggctggg gccaacgtca tccgcttgcc gggccgggtg tacctgggcc gccgacagaa
21841 tggactttct tatacgcacc ccgtaatgag gaagaagcta gggtagtaga aatgattgtc
21901 gaggcaagta tcggatacat gactaatgat ccggcaggta aaatcgtcga gaatgccaaa
21961 tgagcttgtc ctgctttaat gagatatgcg agaagcctat gatcgcatga tatttgcttt
22021 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
22081 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
22141 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
22201 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
22261 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
22321 aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg
22381 acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc
22441 gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc
22501 accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg
22561 cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac
22621 gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagga
22681 ccgctgttag agctcagtg
//
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