pGL4.77[hRlucP/Hygro]

价格:1500元

联系方式:I47-825O-882O

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pGL4.77载体基本信息

出品公司: Promega
载体名称: pGL4.77 ; pGL4.77[hRlucP/Hygro]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5252 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucP
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.77[hRlucP/Hygro]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucP。
产品目录号: E6951
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.77载体质粒图谱和多克隆位点信息

pGL4.77载体图谱



pGL4.77 载体特征

pGL4.77载体简介

The pGL4.77[hRlucP/Hygro] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.77[hRlucP/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.77载体序列

LOCUS       AY864932                5252 bp    DNA     circular SYN 31-JAN-2005
DEFINITION  Luciferase reporter vector pGL4.77[hRlucP/Hygro], complete
            sequence.
ACCESSION   AY864932
VERSION     AY864932.1  GI:58201881
KEYWORDS    .
SOURCE      Luciferase reporter vector pGL4.77[hRlucP/Hygro]
  ORGANISM  Luciferase reporter vector pGL4.77[hRlucP/Hygro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Paguio,A., Almond,B. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Luciferase reporter vector
                     pGL4.77[hRlucP/Hygro]"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:309058"
     misc_feature    1..70
                     /note="multiple cloning site"
     CDS             100..1158
                     /note="Renilla luciferase"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRlucP"
                     /protein_id="AAW66994.1"
                     /db_xref="GI:58201882"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
                     ESGMDRHPAACASARINV"
     misc_feature    1198..1419
                     /note="SV40 late poly A"
     misc_feature    1467..1885
                     /note="SV40 enhancer/promoter"
     CDS             1910..2947
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic hygromycin resistance protein"
                     /protein_id="AAW66995.1"
                     /db_xref="GI:58201883"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV"
     regulatory      2971..3019
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal sequence"
     primer_bind     3086..3105
                     /note="RVprimer4 binding site"
     rep_origin      3343
                     /note="ColEI-derived plasmid replication origin"
     CDS             complement(4134..4994)
                     /note="Ampr"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAW66996.1"
                     /db_xref="GI:58201884"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    5099..5252
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     5201..5220
                     /note="RVprimer3 binding site"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
     1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
     1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
     1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
     1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
     1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc
     1921 cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga
     1981 cctgatgcag ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg
     2041 cggctatgta ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta
     2101 ccgccacttc gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag
     2161 cgagagcctg acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc
     2221 cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc
     2281 cgccgacctc agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta
     2341 cacaacctgg cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac
     2401 cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg
     2461 ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt
     2521 cctgaccgac aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga
     2581 cagtcagtac gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca
     2641 gcagactcgc tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc
     2701 ctacatgctg cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga
     2761 cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt
     2821 cggtcgcaca caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt
     2881 gctggccgac agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg
     2941 agtttaaact ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
     3001 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
     3061 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3121 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4081 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     4141 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     4201 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     4261 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     4321 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     4381 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     4441 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4501 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4561 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     4621 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     4681 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     4741 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     4801 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     4861 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     4921 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     4981 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     5041 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     5101 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     5161 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     5221 cagtgcaagt gcaggtgcca gaacatttct ct
//

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