| 出品公司: | |
|---|---|
| 载体名称: | pSicoR |
| 质粒类型: | 哺乳动物细胞慢病毒表达载体，RNAi, Cre/loxP |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7567 bp |
| 5' 测序引物及序列: | pSicoR-F: TGCAGGGGAAAGAATAGTAGAC |
| 3' 测序引物及序列: | pSicoR-R: CAAACACAGTGCACACCACGC |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: | 载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。 |
| 产品目录号: | |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pSicoR载体允许条件控制（Cre-Lox）,稳定表达shRNAs，能够RNA干扰细胞和转基因小鼠的基因表达。shRNA编码寡核苷序列可以通过HpaI和XhoI限制性内切酶位点插入pSicoR载体。
载体序列
LOCUS pSicoR 7567 bp DNA circular SYN
DEFINITION pSicoR
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..7567
/organism="pSicoR"
/mol_type="other DNA"
promoter 248..824
/label="CMV_immearly_promoter"
misc_feature 327..614
/label="CAG_enhancer"
misc_feature 781..801
/label="CMV_fwd_primer"
misc_feature 844..1024
/label="HIV-1_5_LTR"
misc_feature 844..1024
/label="truncHIV-1_3_LTR"
misc_feature 1135..1179
/label="HIV-1_psi_pack"
CDS 1573..2460
/label="ORF frame 1"
misc_feature 1695..1928
/label="RRE"
misc_feature 2459..2474
/label="cPPT"
misc_feature 2826..2859
/label="loxP"
promoter 3025..3577
/label="CMV_immearly_promoter"
misc_feature 3080..3367
/label="CAG_enhancer"
misc_feature 3534..3554
/label="CMV_fwd_primer"
promoter 3535..3604
/label="CMV_promoter"
CDS complement(3613..4383)
/label="ORF frame 2"
CDS 3628..4347
/label="ORF frame 1"
gene 3631..4344
/label="EGFP"
/gene="EGFP"
misc_feature complement(3673..3694)
/label="EGFP_N_primer"
misc_feature 3811..3840
/label="Y66 (EGFP)"
misc_feature 4281..4302
/label="EGFP_C_primer"
misc_feature 4367..4400
/label="loxP"
misc_feature 4458..5045
/label="WPRE"
CDS 4546..5100
/label="ORF frame 1"
CDS 4559..5599
/label="ORF frame 2"
misc_feature complement(5048..5064)
/label="pBluescriptKS_primer"
misc_feature 5236..5257
/label="U3PPT"
misc_feature 5236..5251
/label="cPPT"
misc_feature 5573..5753
/label="HIV-1_5_LTR"
misc_feature 5573..5753
/label="truncHIV-1_3_LTR"
gene complement(6574..7434)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(6574..7434)
/label="ORF frame 2"
promoter complement(7476..7504)
/label="AmpR_promoter"
ORIGIN
1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
2641 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact
2701 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg
2761 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga
2821 tttctataac ttcgtatagc atacattata cgaagttata catgtcacaa aaggaaactc
2881 accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg agaaaagcct
2941 tgttaacgcg cggtgaccct cgagtactag gatccattag gcggccgcgt ggataaccgt
3001 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
3061 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
3121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
3181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
3241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
3301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
3361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
3421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
3481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
3541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
3601 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
3661 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
3721 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
3781 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
3841 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
3901 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
3961 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
4021 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
4081 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
4141 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
4201 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
4261 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
4321 ctcggcatgg acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag
4381 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat
4441 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
5041 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
5101 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
5161 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
5221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
5281 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
5341 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
5401 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
5461 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
5521 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
5581 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
5641 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
5701 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
5761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
5821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
5881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
5941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
6001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
6061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
6121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
6181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
6241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
6301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
6361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
6421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
6481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
6541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
6601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
6661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
6721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
6841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
6901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
6961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
7021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
7081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
7141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
7201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
7261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
7321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
7381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
7441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
7501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
7561 acctgac
//
