| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pXINSECT-DEST38 |
| 质粒类型: | 昆虫表达载体；Gateway载体 |
| 克隆方法: | Gateway |
| 启动子: | Actin |
| 载体大小: | 12419 kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无标签 |
| 载体抗性: | 氨苄青霉素、氯霉素（仅空载体） |
| 真核筛选标记: | 无 |
| 克隆菌株: | DB3.1 |
| 宿主细胞（系）: | High Five（推荐） 等细胞系 |
| 备注: |
pXINSECT-DEST38载体与pBmA-neo载体共转染，可以建立稳表达细胞系； 应用Gateway技术，可快速高效将 目的基因片段（尤其适用于目的基因片段>1kb，用传统的连接酶法效率低的）插入到载体中； |
| 产品目录号: | 12249-025 |
| 瞬表达/稳表达: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pXINSECT-DEST38载体优势：
Using the Express Insect Kit or the Express Insect Vector Set to achieveprotein expression in insect cells has the following advantages:
Non-lytic, continuous protein expression
Minimal degradation of your protein of interest as compared to viralexpression systems
Higher protein expression level achieved than other similar non-viral insectexpression systems
Choice of transient or stable protein expression
Adapted for use with the Gateway Technology or traditional restrictionenzyme-mediated cloning
载体特征：
pXINSECT-DEST38 and pXINSECT-DEST39 have the following features:
The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
Chloramphenicol resistance gene located between the two attR sites for counterselection
The ccdB gene located between the two attR sites for negative selection
The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
Ampicillin resistance gene for selection of transformants in E. coli
The pUC origin for high copy replication and maintenance of the plasmid in E. coli
载体序列
ATGTATAATTAATTAAATTCAAATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGT CGGACAACGGAACCAGACCCTGTAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCT CTCTCTTCGGTTCACTTTACTGATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGA CTAGCGTCTACATCTTTAATCTCGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACC GCTCTTCGTTTTTGAAAGACAATACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATA CTGTTCGACAATGTGCATGCTGCCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTC GCAATATTGTCATGATATTTATAATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCG TACTTTTTTTGTTAACAATTAAATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTT TACACTTTCCGAATACTTTAATATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCA ATTTCATTACTCTCTTTACGACTAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGT ACAACTTGCTCAAAAGAAACCCACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTG CGCGTAATACATATCTAAATTAAAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAG TGGCATTTTTTACAATTTTGTGCAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGC CGGTTTCTTTAACCAAATTGTACGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATT ATCTACCATGTAATAGTTGTTTGTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCA AACGGCTTTACTTCACTTTTTTTAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACC TATTGGAATTGTAGTCGTTGTCGTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGT AATTTCTTGAGTTGAAGCAACCGTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAG CTTTGAATTGTGCTTTTCTTGTATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTG AATCGTCCAAGTATTCGTCCAGCTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATG CTCAAAAGATTCCGCATTATAAGCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCC GCATTATAAGCAGAACTAACTGCTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGT TATCATCAGTTAACGAATTGACGCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTAC CGTGTCGGCTCCATCCGGCGTGGGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAA AACTCTGAATAGCCGTTGTCGAACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAAT TAATTTGCGTCATAGTCGTTTGGTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAAT ATTCAACATGAACGTCAATTTATACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCC TCTAAAACCAATGCGGTTATCGTTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTA ATGCAAAAACTCGTTTGGTGAGCGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAAC ACCGTCCTTGAACGGAACACGAGGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGAT TTATTTACATACAACAAGTACACTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAG TGTGGCACATTTTGTCTGTGCGCAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGC GCACACGGACAACGGACCCGACAAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTG CATTTTATTAATAACTTACAAAATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCC CGAACTGCAAATCGATCGATAAGCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAAT TAATTTCTCCGCGTCGTATTATACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATG ATTTTGATGATGGAGGTGCGCTCAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCA ATTTGCTACGACGCAATTATTGTGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAA AGTATATTGATATTAAATCATATAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATT AAAATTGTTCAACGATTGTATGAGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATT CTACTTGTAAAACACAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTT AAAACTAAACTCGCTTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTT TTCAAAACTGAACCGGCTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATT AAACTGATGTCATTTTTATACACGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAAC TCGCTTTACAAGTAGAATTCTACTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTG TTTTCGTCATAAAATGTTTATACAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATT CTACGTTACATTTAAATTCGTTGTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAAC GTGCAACCAACGATTGTATTTGACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGC ACGGCTTCTTTTTTTACCACTATCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACAT AAGCGTACGCGTTTAAAACGTGCGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTT GCGTAACTCGTTTAAAGCATTAATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGA ACCGAGTAGTGGCATGAATAAAATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCG CGGGTAGGTACCACCACCCTGCCTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGC GGCCGTGGTACTGAGACCTTAGAACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATG GGCTCCAGTAACCACTTAACATCAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAA AATAAATATGTTTGAAGTCCGTAACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCT TCACCGACAATGGAAAATAATCACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAG TTTCGATAAAGCGAGGCTACCAAGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACA ACTTTTACCTCTCAAATTGCCCACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAG CCGCTACGATATCATTATCATACAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATC TCTTCATAATTATAATTAAAAAGAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGA GATTAATTAACCGGATTTTCTGGGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGA AATTCTATGAAACTTCGGCGGGAGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCA GTTATCAAGGCATCGCCTTATCAAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAA ACTGAGTCAGCCCGCGATTGGTGGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCT TTTATATAGTTTATCCTCACGAGTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATC TACGTACTTGTCACTTATTTAATAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACA TATTTATTTATTTGTAAAATTTGAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTT TGAGTGTTTTTTTTTTTTAATAATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGT GAAGTGAAATTTTTTATAAAAAAAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTAC TAAATAAGAAATAAGTTTATTGGACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGA AATTGGGAACAGTGAATTATGAATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAA ATTTGTCGTTTACTTAGTATAATGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCA GACTAATTCAAGAGGTGCGACGAAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCG AATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAA TGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAA CATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA TGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATC ACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTG CTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAA GCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATG GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAA CTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCA AGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTC TCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCC CCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCA TCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG CAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTG ATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAG CAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTC CGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAAT CAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACT GGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGA GTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAA AGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATG GCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAA ACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCG ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAA TATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATCGACCCGGGT CTAGAGCGGCCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAACGAAAGA TTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACGAAACCA CATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATTGTCCGG TGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCATCGACA ATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCCTCGCCT CCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCATTGTACA CAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGGTGGCTC AAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATATGCATA TTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTAAAGTTA CTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACTTGCTGT CCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGACAGTTA CGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGAATGCGA GTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTACTTTCT ACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTAGAGGTA CCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACACACAAG ATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCATTGCGT AGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTTAGTGAG GGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG GCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCA GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCG CCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTA GATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCG AGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGC CAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGG CAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAAC CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTAC TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACA CGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCA TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA AGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCT CATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTT GAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAA ACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCC GTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGT GGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTG CGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGC TGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT GAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATT GCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACGTTGAAGTACCTTACACAAC AAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCCAAAATCTAAAGCAAGGAGA AGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGCAATTTTGCAAAACTTTACT GTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAATCTTCGCAGAGTCAATAAAT TGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAAGGCACTCCCGCCGGTTTCA CAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATGGGCCGGGCTATCAATTATC TAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAACGATGTCCCCAACATACGC GGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTGCGGCGCATGGACCAACGTG CCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAAAACTTCCCGGAGACCAACG TGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTTACATAATCACATGAGAACC TTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTTGTTTACCGCCGCCACATTA GTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTGCAAGGTAGAAACGCCGGAG AAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCAATCTAGCTCAGTCGGATGT TAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAACCAACATGTGCCAAGGATTT AACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAACCCAACTTCGCCTCAATACG TAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTACACGTTTAGTGATTTAATT GGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAATCGTCCAACGGTAGTGACA GCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTGTTTTTAGGTTTAATACTTT ATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTGGGGCACCAACTCCCATTGT TGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAAGACAAAAATAATATAAAAT ATATGTATAATTAATTAAATTCAAAAGAT
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