pXINSECT-DEST38

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pXINSECT-DEST38
质粒类型: 昆虫表达载体;Gateway载体
克隆方法: Gateway
启动子: Actin
载体大小: 12419 kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 无标签
载体抗性: 氨苄青霉素、氯霉素(仅空载体)
真核筛选标记:
克隆菌株: DB3.1
宿主细胞(系): High Five(推荐) 等细胞系
备注: pXINSECT-DEST38载体与pBmA-neo载体共转染,可以建立稳表达细胞系;
应用Gateway技术,可快速高效将 目的基因片段(尤其适用于目的基因片段>1kb,用传统的连接酶法效率低的)插入到载体中;
产品目录号: 12249-025
瞬表达/稳表达: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pXINSECT-DEST38 载体图谱



pXINSECT-DEST38 多克隆位点

pXINSECT-DEST38 载体特征

载体简介


pXINSECT-DEST38载体优势:
Using the Express Insect Kit or the Express Insect Vector Set to achieveprotein expression in insect cells has the following advantages:
 Non-lytic, continuous protein expression
 Minimal degradation of your protein of interest as compared to viralexpression systems
 Higher protein expression level achieved than other similar non-viral insectexpression systems
 Choice of transient or stable protein expression
 Adapted for use with the Gateway Technology or traditional restrictionenzyme-mediated cloning

载体特征:
pXINSECT-DEST38 and pXINSECT-DEST39 have the following features:
 The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
 Chloramphenicol resistance gene located between the two attR sites for counterselection
 The ccdB gene located between the two attR sites for negative selection
 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
 Ampicillin resistance gene for selection of transformants in E. coli
 The pUC origin for high copy replication and maintenance of the plasmid in E. coli

载体序列

ATGTATAATTAATTAAATTCAAATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGT
CGGACAACGGAACCAGACCCTGTAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCT
CTCTCTTCGGTTCACTTTACTGATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGA
CTAGCGTCTACATCTTTAATCTCGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACC
GCTCTTCGTTTTTGAAAGACAATACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATA
CTGTTCGACAATGTGCATGCTGCCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTC
GCAATATTGTCATGATATTTATAATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCG
TACTTTTTTTGTTAACAATTAAATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTT
TACACTTTCCGAATACTTTAATATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCA
ATTTCATTACTCTCTTTACGACTAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGT
ACAACTTGCTCAAAAGAAACCCACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTG
CGCGTAATACATATCTAAATTAAAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAG
TGGCATTTTTTACAATTTTGTGCAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGC
CGGTTTCTTTAACCAAATTGTACGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATT
ATCTACCATGTAATAGTTGTTTGTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCA
AACGGCTTTACTTCACTTTTTTTAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACC
TATTGGAATTGTAGTCGTTGTCGTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGT
AATTTCTTGAGTTGAAGCAACCGTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAG
CTTTGAATTGTGCTTTTCTTGTATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTG
AATCGTCCAAGTATTCGTCCAGCTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATG
CTCAAAAGATTCCGCATTATAAGCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCC
GCATTATAAGCAGAACTAACTGCTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGT
TATCATCAGTTAACGAATTGACGCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTAC
CGTGTCGGCTCCATCCGGCGTGGGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAA
AACTCTGAATAGCCGTTGTCGAACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAAT
TAATTTGCGTCATAGTCGTTTGGTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAAT
ATTCAACATGAACGTCAATTTATACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCC
TCTAAAACCAATGCGGTTATCGTTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTA
ATGCAAAAACTCGTTTGGTGAGCGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAAC
ACCGTCCTTGAACGGAACACGAGGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGAT
TTATTTACATACAACAAGTACACTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAG
TGTGGCACATTTTGTCTGTGCGCAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGC
GCACACGGACAACGGACCCGACAAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTG
CATTTTATTAATAACTTACAAAATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCC
CGAACTGCAAATCGATCGATAAGCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAAT
TAATTTCTCCGCGTCGTATTATACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATG
ATTTTGATGATGGAGGTGCGCTCAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCA
ATTTGCTACGACGCAATTATTGTGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAA
AGTATATTGATATTAAATCATATAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATT
AAAATTGTTCAACGATTGTATGAGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATT
CTACTTGTAAAACACAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTT
AAAACTAAACTCGCTTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTT
TTCAAAACTGAACCGGCTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATT
AAACTGATGTCATTTTTATACACGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAAC
TCGCTTTACAAGTAGAATTCTACTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTG
TTTTCGTCATAAAATGTTTATACAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATT
CTACGTTACATTTAAATTCGTTGTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAAC
GTGCAACCAACGATTGTATTTGACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGC
ACGGCTTCTTTTTTTACCACTATCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACAT
AAGCGTACGCGTTTAAAACGTGCGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTT
GCGTAACTCGTTTAAAGCATTAATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGA
ACCGAGTAGTGGCATGAATAAAATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCG
CGGGTAGGTACCACCACCCTGCCTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGC
GGCCGTGGTACTGAGACCTTAGAACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATG
GGCTCCAGTAACCACTTAACATCAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAA
AATAAATATGTTTGAAGTCCGTAACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCT
TCACCGACAATGGAAAATAATCACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAG
TTTCGATAAAGCGAGGCTACCAAGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACA
ACTTTTACCTCTCAAATTGCCCACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAG
CCGCTACGATATCATTATCATACAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATC
TCTTCATAATTATAATTAAAAAGAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGA
GATTAATTAACCGGATTTTCTGGGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGA
AATTCTATGAAACTTCGGCGGGAGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCA
GTTATCAAGGCATCGCCTTATCAAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAA
ACTGAGTCAGCCCGCGATTGGTGGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCT
TTTATATAGTTTATCCTCACGAGTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATC
TACGTACTTGTCACTTATTTAATAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACA
TATTTATTTATTTGTAAAATTTGAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTT
TGAGTGTTTTTTTTTTTTAATAATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGT
GAAGTGAAATTTTTTATAAAAAAAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTAC
TAAATAAGAAATAAGTTTATTGGACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGA
AATTGGGAACAGTGAATTATGAATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAA
ATTTGTCGTTTACTTAGTATAATGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCA
GACTAATTCAAGAGGTGCGACGAAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCG
AATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAA
TGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAA
CATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA
TGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATC
ACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTG
CTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAA
GCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATG
GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAA
CTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCA
AGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTC
TCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCC
CCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCA
TCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG
CAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTG
ATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAG
CAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTC
CGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAAT
CAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACT
GGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGA
GTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAA
AGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATG
GCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAA
ACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCG
ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAA
TATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATCGACCCGGGT
CTAGAGCGGCCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAACGAAAGA
TTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACGAAACCA
CATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATTGTCCGG
TGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCATCGACA
ATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCCTCGCCT
CCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCATTGTACA
CAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGGTGGCTC
AAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATATGCATA
TTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTAAAGTTA
CTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACTTGCTGT
CCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGACAGTTA
CGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGAATGCGA
GTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTACTTTCT
ACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTAGAGGTA
CCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACACACAAG
ATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCATTGCGT
AGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTTAGTGAG
GGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT
TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA
TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG
GCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG
CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCA
GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT
CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT
TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCG
CCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC
CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT
TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTA
GATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT
TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC
TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCG
AGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT
GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGC
CAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT
GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG
GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGG
CAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAAC
CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC
GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA
TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTAC
TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACA
CGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCA
TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA
AGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCT
CATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTT
GAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAA
ACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCC
GTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGT
GGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTG
CGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGC
TGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG
TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT
GAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATT
GCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACGTTGAAGTACCTTACACAAC
AAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCCAAAATCTAAAGCAAGGAGA
AGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGCAATTTTGCAAAACTTTACT
GTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAATCTTCGCAGAGTCAATAAAT
TGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAAGGCACTCCCGCCGGTTTCA
CAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATGGGCCGGGCTATCAATTATC
TAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAACGATGTCCCCAACATACGC
GGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTGCGGCGCATGGACCAACGTG
CCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAAAACTTCCCGGAGACCAACG
TGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTTACATAATCACATGAGAACC
TTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTTGTTTACCGCCGCCACATTA
GTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTGCAAGGTAGAAACGCCGGAG
AAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCAATCTAGCTCAGTCGGATGT
TAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAACCAACATGTGCCAAGGATTT
AACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAACCCAACTTCGCCTCAATACG
TAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTACACGTTTAGTGATTTAATT
GGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAATCGTCCAACGGTAGTGACA
GCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTGTTTTTAGGTTTAATACTTT
ATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTGGGGCACCAACTCCCATTGT
TGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAAGACAAAAATAATATAAAAT
ATATGTATAATTAATTAAATTCAAAAGAT

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