| 质粒名称: | FUdeltaGW-rtTA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 19780 |
| 存储实验室: | Konrad Hochedlinger |
|
载体骨架基本信息 |
|
| 载体名称: | FUdeltaGW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 宿主菌： | Stbl3 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | reverse tetracycline transactivator,rtTA |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | 1025 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI（如果已销毁，则未知） |
| 3' 克隆位点: | EcoRI（如果已销毁，则未知） |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | FUdeltaGW-rtTA was a gift from Konrad Hochedlinger (Addgene plasmid # 19780 ; http://n2t.net/addgene:19780 ; RRID:Addgene_19780) |
| 参考文献部分: | A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420 |
买家导航
FUdeltaGWrtTA载体基本信息
FUdeltaGWrtTA载体图谱质粒图谱和多克隆位点信息
FUdeltaGWrtTA载体简介
FUdeltaGWrtTA载体序列
LOCUS FUdeltaGW-rtTA 9959 bp ds-DNA circular SYN 16-AUG-2021
DEFINITION 3rd generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS FUdeltaGW-rtTA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9959)
AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger
K
TITLE A high-efficiency system for the generation and study of human
induced pluripotent stem cells.
JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
PUBMED 18786420
REFERENCE 2 (bases 1 to 9959)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9959
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2622..3833
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3670..3689
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3876..4622
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 4651..5239
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4704..4724)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5122..5133)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5242..5258)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5243..5259)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5764..5944
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5970..5987)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5976..6200
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6246..6674
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6333..6352)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6543..6564
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6683..6703)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6688..7017
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6868..7003
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6930..6949
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7065..7112
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7131..7505
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7635..7756
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7672..7691)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7726..7745
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7805..7821)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7805..7821)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7818..7840)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7829..7845
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7853..7883)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7898..7919
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8036..8053)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8207..8795)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8287..8306)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8966..9826)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9589..9608
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9827..9931)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
3841 gggctgcagg tcgactctag aattaattcg ccaccatgtc tagactggac aagagcaaag
3901 tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
3961 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
4021 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
4081 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg
4141 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
4201 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
4261 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
4321 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
4381 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
4441 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg
4501 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
4561 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt
4621 aaactagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
4681 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
4741 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
4801 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
4861 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
4921 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
4981 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
5041 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
5101 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
5161 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
5221 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
5281 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
5341 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
5401 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
5461 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
5521 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
5581 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
5641 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
5701 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
5761 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
5821 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
5881 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
5941 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
6001 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
6061 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
6121 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
6181 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
6241 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
6301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
6361 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
6421 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
6481 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
6541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
6601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
6661 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
6721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
6781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
6841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
6901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
6961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
7021 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
7081 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
7141 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
7201 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
7261 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
7321 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
7381 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
7441 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
7501 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
7561 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
7621 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
7681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
7741 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
7801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
7861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
7921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
7981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
8641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
8881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
8941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
9001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
9061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
9121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
9181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
9241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
9301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
9361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
9421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
9481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
9541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
9601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
9661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
9721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
9781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
9841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
9901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
