pFLAG-CMV-5a

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: SIGMA
载体名称: pFLAG-CMV-5a
质粒类型: 哺乳动物表达载体
高拷贝/低拷贝: 低拷贝
克隆方法: 多克隆位点;限制性内切酶
启动子: CMV
载体大小: 4.7 kb
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: FLAG tag (C-末端)
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: --
宿主细胞(系): 常用细胞系,如293、Hela等
备注: CMV启动子驱动目的蛋白高水平表达;
FLAG标签含8个氨基酸残基(DYKDDDDK)
一般位于融合蛋白的外表面,具有高度免疫灵敏性,便于检测和纯化目的蛋白;
产品目录号: E7523
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pFLAG-CMV-5a 多克隆位点

pFLAG-CMV-5a 载体特征

载体简介

Product Description
Versions of the pFLAG-CMV-5 expression vector for the three translational reading frames preceding the FLAG coding sequence are provided.

pFLAG-CMV-5a expression vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of C-terminal FLAG fusion proteins in mammalian cells.

The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs.
The multiple cloning region of the pFLAG-CMV-5a expression vector is not preceded by a translational initiation signal. A translational start sequence3 must be included with the insert DNA.
pFLAG-CMV-5a expression vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.


p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.

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