pQE-TriSystem

价格:1800元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Qiagen
载体名称: pQE-TriSystem
质粒类型: 原核表达载体;昆虫表达载体;哺乳细胞表达载体
高拷贝/低拷贝: 高拷贝
启动子: P10启动子
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5766 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 8xHis
载体抗性: 氨苄青霉素
筛选标记: --
备注: 高水平蛋白表达
产品目录号: --
稳定性: 瞬时表达
组成型: 非组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pQE-TriSystem载体图谱



pQE-TriSystem多克隆位点1

pQE-TriSystem多克隆位点2

pQE-TriSystem载体特征

载体简介

载体序列

LOCUS       pQE-TriSystem	5766 bp 	DNA   circular	SYN
DEFINITION  pQE-TriSystem
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="pQE-TriSystem"
                     /mol_type="other DNA"
     misc_feature    68..355
                     /label="CAG_enhancer"
     CDS             complement(456..983)
                     /label="ORF frame 2"
     misc_feature    1012..1031
                     /label="pCAG_F_primer"
     terminator      1743..1778
                     /label="T7_terminator"
     misc_feature    complement(1801..1818)
                     /label="Baculovirs_rev_primer"
     misc_feature    1808..1885
                     /label="3_AcPH"
     CDS             complement(1915..3168)
                     /label="ORF frame 1"
     misc_feature    complement(1997..2795)
                     /label="r_ORF1629"
     gene            complement(4015..4875)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4015..4875)
                     /label="ORF frame 1"
     CDS             complement(5103..5708)
                     /label="ORF frame 2"
ORIGIN
    1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
   61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
  121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
  181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
  241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
  301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
  361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
  421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
  481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA
  541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG
  601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC
  661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG
  721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA
  781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG
  841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT
  901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
  961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
 1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA
 1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC
 1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
 1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA
 1261 TATACCATGG CGATATCCCG GGAGCTCGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG
 1321 CAGGTCGACG GTACCGGTTC GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCCAG
 1381 CCAGAACTCG CCCCGGAAGA CCCCGAGGAT CTCGAGCACC ACCATCACCA TCACCATCAC
 1441 TAAGTGATTA ACCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG
 1501 CCCTGGCTCA CAAATACCAC TGAGATCGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC
 1561 ATCATGAAGC CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA
 1621 ATAGTGTGTT GGAATTTTTT GTGTCTCTCA CTCGGAAGGA CATATGGGAG GGCAAATCAT
 1681 TTAAAACATC AGAATGAGTT TTTGGTTTAG AGTTTGGCAA CATATGCCCA TATGTAACTA
 1741 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGCATGCGGA
 1801 GGAAATTCTC CTTGAAGTTT CCCTGGTGTT CAAAGTAAAG GAGTTTGCAC CAGACGCACC
 1861 TCTGTTCACT GGTCCGGCGT ATTAAAACAC GATACATTGT TATTAGTACA TTTATTAAGC
 1921 GCTAGATTCT GTGCGTTGTT GATTTACAGA CAATTGTTGT ACGTATTTTA ATAATTCATT
 1981 AAATTTATAA TCTTTAGGGT GGTATGTTAG AGCGAAAATC AAATGATTTT CAGCGTCTTT
 2041 ATATCTGAAT TTAAATATTA AATCCTCAAT AGATTTGTAA AATAGGTTTC GATTAGTTTC
 2101 AAACAAGGGT TGTTTTTCCG AACCGATGGC TGGACTATCT AATGGATTTT CGCTCAACGC
 2161 CACAAAACTT GCCAAATCTT GTAGCAGCAA TCTAGCTTTG TCGATATTCG TTTGTGTTTT
 2221 GTTTTGTAAT AAAGGTTCGA CGTCGTTCAA AATATTATGC GCTTTTGTAT TTCTTTCATC
 2281 ACTGTCGTTA GTGTACAATT GACTCGACGT AAACACGTTA AATAGAGCTT GGACATATTT
 2341 AACATCGGGC GTGTTAGCTT TATTAGGCCG ATTATCGTCG TCGTCCCAAC CCTCGTCGTT
 2401 AGAAGTTGCT TCCGAAGACG ATTTTGCCAT AGCCACACGA CGCCTATTAA TTGTGTCGGC
 2461 TAACACGTCC GCGATCAAAT TTGTAGTTGA GCTTTTTGGA ATTATTTCTG ATTGCGGGCG
 2521 TTTTTGGGCG GGTTTCAATC TAACTGTGCC CGATTTTAAT TCAGACAACA CGTTAGAAAG
 2581 CGATGGTGCA GGCGGTGGTA ACATTTCAGA CGGCAAATCT ACTAATGGCG GCGGTGGTGG
 2641 AGCTGATGAT AAATCTACCA TCGGTGGAGG CGCAGGCGGG GCTGGCGGCG GAGGCGGAGG
 2701 CGGAGGTGGT GGCGGTGATG CAGACGGCGG TTTAGGCTCA AATGTCTCTT TAGGCAACAC
 2761 AGTCGGCACC TCAACTATTG TACTGGTTTC GGGCGCCGTT TTTGGTTTGA CCGGTCTGAG
 2821 ACGAGTGCGA TTTTTTTCGT TTCTAATAGC TTCCAACAAT TGTTGTCTGT CGTCTAAAGG
 2881 TGCAGCGGGT TGAGGTTCCG TCGGCATTGG TGGAGCGGGC GGCAATTCAG ACATCGATGG
 2941 TGGTGGTGGT GGTGGAGGCG CTGGAATGTT AGGCACGGGA GAAGGTGGTG GCGGCGGTGC
 3001 CGCCGGTATA ATTTGTTCTG GTTTAGTTTG TTCGCGCACG ATTGTGGGCA CCGGCGCAGG
 3061 CGCCGCTGGC TGCACAACGG AAGGTCGTCT GCTTCGAGGC AGCGCTTGGG GTGGTGGCAA
 3121 TTCAATATTA TAATTGGAAT ACAAATCGTA AAAATCTGCT ATAAGCATTG TAATTTCGCT
 3181 ATCGTTTACC GTGCCGATAT TTAACAACCG CTCAATGTAA GCAATTGTAT TGTAAAGAGA
 3241 TTGTCTCAAG CTCGGAACGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
 3301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
 3361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
 3421 TAGGCTCCGC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
 3481 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
 3541 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
 3601 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
 3661 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
 3721 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
 3781 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
 3841 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
 3901 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
 3961 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGTTACCA
 4021 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
 4081 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
 4141 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
 4201 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
 4261 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
 4321 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
 4381 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
 4441 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
 4501 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
 4561 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
 4621 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
 4681 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
 4741 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
 4801 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
 4861 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
 4921 TCTCATGTCC GCGCGTTTCC TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC
 4981 AAACTGCCAA AAAATGAAAA CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG
 5041 TCTCAATCCT ATTTGTAATT ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT
 5101 TATTAACGAT ACAAACCAAA CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC
 5161 GCGTAGTTAT AATCGCTGAG GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT
 5221 TGCGACAATA TAATTTTATT TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT
 5281 TCCTTCTCTT TTTCATTTTT CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT
 5341 ATGTATCTAT CGTATAGAGT AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG
 5401 GGGTGTATAG TACCGCTGCG CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT
 5461 AGTTCTTCGG AGTGTGTTGC TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG
 5521 TCGGTTTTGT ACAATATGTT GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA
 5581 TTTTTTAGCA GCACCGGATT AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA
 5641 AACAGTTCAC CTCCCTTTTC TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA
 5701 ACAGCCATTG TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA
 5761 TAGTTG
//

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