| 质粒名称: | BASIC_SEVA_17_pKD46.10 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 178917 |
| 存储实验室: | Paul Freemont |
|
载体骨架基本信息 |
|
| 载体名称: | BASIC_SEVA_17_pKD46.10 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2910 bp |
| 完整质粒大小: | 3984 bp |
| 载体类型: | Synthetic Biology |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB 5-alpha |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Scarlet counter-selection cassette |
| 物种来源: | Synthetic |
| 插入大小: | 1074bp |
| 克隆方法: | 未知 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | ggcggcggatttgtcctac |
| 3’测序引物 |
ggtgagaatccaggggtcc
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | BASIC_SEVA_17_pKD46.10 was a gift from Paul Freemont (Addgene plasmid # 178917 ; http://n2t.net/addgene:178917 ; RRID:Addgene_178917) |
| 参考文献部分: | basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method. Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont P, Storch M. Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 10.1093/synbio/ysac023. eCollection 2022. 10.1093/synbio/ysac023 PubMed 36381610 |
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BASIC_SEVA_17_pKD46.10载体质粒基本信息
BASIC_SEVA_17_pKD46.10质粒图谱载体图谱和BASIC_SEVA_17_pKD46.10载体序列质粒序列多克隆位点信息
BASIC_SEVA_17_pKD46.10质粒载体简介
BASIC_SEVA_17_pKD46.10质粒序列载体序列
LOCUS BASIC_SEVA_17_pKD46.10 3984 bp DNA circular SYN 14-APR-2025
DEFINITION BASIC DNA assembly vector.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3984)
AUTHORS Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont
P, Storch M
TITLE basicsynbio and the BASIC SEVA collection: software and vectors for
an established DNA assembly method.
JOURNAL Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi:
10.1093/synbio/ysac023. eCollection 2022.
PUBMED 36381610
REFERENCE 2 (bases 1 to 3984)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol
(Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: "
10.1093/synbio/ysac023. eCollection 2022"
COMMENT Sequence Label: BASIC_SEVA_17_pKD46.10
FEATURES Location/Qualifiers
source 1..3984
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 68..162
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
promoter 235..326
/gene="bla"
/label=AmpR promoter
CDS join(327..395,396..1187)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
terminator 1191..1230
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS complement(1358..2308)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 2356..2578
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
terminator complement(2861..2947)
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
promoter 3111..3145
/label=J23119 promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119)"
misc_RNA 3148..3197
/label=sTRSV HHRz
/note="hammerhead ribozyme from the tobacco ringspot virus
satellite RNA (Khvorova et al., 2003)"
RBS 3263..3271
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
1261 tctcgtacaa ttctgaggtc cacacagata gtgtcctgtt ctacttttgt ttgttagtct
1321 tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg tatttagcca
1381 gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat
1441 catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg
1501 cagttaaagc atcgtgtagt gtttttctta gtccgttacg taggtaggaa tctgatgtaa
1561 tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga
1621 gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct
1681 tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa
1741 cccattggtt aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa
1801 attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta
1861 ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat
1921 tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta
1981 attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt
2041 taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta
2101 atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga
2161 acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac
2221 agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat
2281 ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac
2341 tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg
2401 gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg
2461 taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata
2521 gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac
2581 tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac
2641 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg
2701 caatcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
2761 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
2821 gcactacagg cgcggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
2881 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
2941 ttatttgatg cctttaatta aggctcggga gacctatcgg taataacagt ccaatctggt
3001 gtaacttcgg aatcgtcccc aattattgaa cacccttcgg ggtgtttttt tgtttctggt
3061 ctaccatctc gttgtgataa tagacctgaa gtgcctactc tggaaaatct ttgacagcta
3121 gctcagtcct aggtataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt
3181 ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa ggctcgctac gagaagattg
3241 ttactttcgc agcgttatta tctaaggagg tagtccatgg ttagcaaagg cgaggcggtt
3301 atcaaggagt ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc
3361 gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa
3421 gtgaccaagg gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat
3481 ggcagccgtg cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc
3541 ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt
3601 acccaggaca ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc
3661 aactttccgc cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc
3721 gagcgtctgt atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg
3781 aaggacggtg gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg
3841 caaatgccgg gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat
3901 tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac
3961 gaactgtata agggatccta ataa
//
