| 出品公司: | Addgene |
|---|---|
| 载体名称: | FUW-tetO-hOKMS |
| 质粒类型: | 慢病毒载体；四环素调控载体；Tet-On系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | EcoRI |
| 启动子: | TRE/Minimal CMV |
| 载体大小: | 13399 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | bleomycin, phleomycin, and Zeocin? |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 大部分哺乳动物细胞 |
| 备注: | FUW-tetO-hOKMS vector expressing the reverse tetracycline transactivator. Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2. |
| 产品目录号: | #51543 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
FUW-tetO-hOKMS载体质粒基本信息
FUW-tetO-hOKMS质粒图谱载体图谱和FUW-tetO-hOKMS载体序列质粒序列多克隆位点信息
FUW-tetO-hOKMS质粒载体简介
FUW-tetO-hOKMS质粒序列载体序列
LOCUS FUW-tetO-hOKMS 13399 bp DNA SYN
DEFINITION FUW-tetO-hOKMS
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..13399
/organism="FUW-tetO-hOKMS"
/mol_type="other DNA"
promoter 8..584
/label="CMV_immearly_promoter"
misc_feature 87..374
/label="CAG_enhancer"
misc_feature 541..561
/label="CMV_fwd_primer"
misc_feature 604..784
/label="HIV-1_5_LTR"
misc_feature 604..784
/label="truncHIV-1_3_LTR"
misc_feature 895..939
/label="HIV-1_psi_pack"
CDS 1327..2214
/label="ORF frame 1"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
misc_feature 1449..1682
/label="RRE"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
misc_feature 2213..2228
/label="cPPT"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
misc_feature 2371..2688
/label="TRE"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
promoter 2689..2808
/label="CMV2_promoter"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
misc_feature 2720..2739
/label="pCEP_fwd_primer"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
misc_feature 2722..2746
/label="LNCX_primer"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSALRAPILQTNGSIHPQF*"
CDS 2869..7827
/label="ORF frame 1"
/translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGG
PGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVG
VESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITL
GYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQ
EICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRV
WFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALY
SSVPFPEGEAFPPVSVTTLGSPMHSNGSGEGRGSLLTCGDVEENPGPMAVSDALLPSF
STFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGG
AGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSP
SSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVS
PSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSK
GSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQ
LPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQEL
MPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPY
HCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFGSGAT
NFSLLKQAGDVEENPGPMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQ
PPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQL
EMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARK
DSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSA
FSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAP
GKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVR
VLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEK
APKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCAGSGQCTNY
ALLKLAGDVESNPGPMYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKR
PMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALH
MKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDS
YAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYM
NGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMIS
MYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM*"
CDS complement(4369..5220)
/label="ORF frame 2"
/translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
RRRRRGG*"
misc_feature 6715..6744
/label="Myc"
/translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
RRRRRGG*"
misc_feature 7862..8449
/label="WPRE"
/translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
RRRRRGG*"
CDS 7950..8999
/label="ORF frame 3"
/translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
LWAASPHRYRRPRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMT
YKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLT
FGWCYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMAR
ELHPDCTGSLWLDQI*"
CDS 7963..8475
/label="ORF frame 1"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature complement(8452..8468)
/label="pBluescriptKS_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 8636..8657
/label="U3PPT"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 8636..8651
/label="cPPT"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 8973..9153
/label="HIV-1_5_LTR"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 8973..9153
/label="truncHIV-1_3_LTR"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature complement(9179..9196)
/label="BGH_rev_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
terminator 9182..9409
/label="bGH_PA_terminator"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
rep_origin 9472..9778
/label="f1_origin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature complement(9892..9912)
/label="pBABE_3_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature complement(9898..10113)
/label="SV40_enhancer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
promoter 9910..10178
/label="SV40_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
rep_origin 10077..10154
/label="SV40_origin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 10139..10158
/label="SV40pro_F_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
promoter 10272..10339
/label="EM7_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 10340..10714
/label="sh_ble"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
gene 10340..10711
/label="bleo"
/gene="bleo"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
terminator 10847..10966
/label="SV40_PA_terminator"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature 10935..10954
/label="EBV_rev_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
promoter complement(11010..11028)
/label="M13_reverse_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
misc_feature complement(11027..11049)
/label="M13_pUC_rev_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
promoter complement(11063..11092)
/label="lac_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
gene complement(12175..13035)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTVDLET*"
CDS complement(12175..13035)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(13077..13105)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
2821 attcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggTat ggcgggacac
2881 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
2941 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
3001 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
3061 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
3121 gtgccccaag gcggcttgga gacctctcag cctgagggTg aagcaggagt cggggtggag
3181 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
3241 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
3301 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
3361 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
3421 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
3481 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
3541 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
3601 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
3661 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
3721 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
3781 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
3841 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
3901 tttccccctg tctcTgtcac cactctgggc tctcccatgc aTtcaaacGG ATCTGGAgag
3961 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc catggctgtc
4021 agcgacgcgc tgctcccatc tttctccacg ttcgcgtctg gcccggcggg aagggagaag
4081 acactgcgtc aagcaggtgc cccgaataac cgctggcggg aggagctctc ccacatgaag
4141 cgacttcccc cagtgcttcc cggccgcccc tatgacctgg cggcggcgac cgtggccaca
4201 gacctggaga gcggcggagc cggtgcggct tgcggcggta gcaacctggc gcccctacct
4261 cggagagaga ccgaggagtt caacgatctc ctggacctgg actttattct ctccaattcg
4321 ctgacccatc ctccggagtc agtggccgcc accgtgtcct cgtcagcgtc agcctcctct
4381 tcgtcgtcgc cgtcgagcag cggccctgcc agcgcgccct ccacctgcag cttcacctat
4441 ccgatccggg ccgggaacga cccgggcgtg gcgccgggcg gcacgggcgg aggcctcctc
4501 tatggcaggg agtccgctcc ccctccgacg gctcccttca acctggcgga catcaacgac
4561 gtgagcccct cgggcggctt cgtggccgag ctcctgcggc cagaattgga cccggtgtac
4621 attccgccgc agcagccgca gccgccaggt ggcgggctga tgggcaagtt cgtgctgaag
4681 gcgtcgctga gcgcccctgg cagcgagtac ggcagcccgt cggtcatcag cgtcagcaaa
4741 ggcagccctg acggcagcca cccggtggtg gtggcgccct acaacggcgg gccgccgcgc
4801 acgtgcccca agatcaagca ggaggcggtc tcttcgtgca cccacttggg cgctggaccc
4861 cctctcagca atggccaccg gccggctgca cacgacttcc ccctggggcg gcagctcccc
4921 agcaggacta ccccgaccct gggtcttgag gaagtgctga gcagcaggga ctgtcaccct
4981 gccctgccgc ttcctcccgg cttccatccc cacccggggc ccaattaccc atccttcctg
5041 cccgatcaga tgcagccgca agtcccgccg ctccattacc aagagctcat gccacccggt
5101 tcctgcatgc cagaggagcc caagccaaag aggggaagac gatcgtggcc ccggaaaagg
5161 accgccaccc acacttgtga ttacgcgggc tgcggcaaaa cctacacaaa gagttcccat
5221 ctcaaggcac acctgcgaac ccacacaggt gagaaacctt accactgtga ctgggacggc
5281 tgtggatgga aattcgcccg ctcagatgaa ctgaccaggc actaccgtaa acacacgggg
5341 caccgcccgt tccagtgcca aaaatgcgac cgagcatttt ccaggtcgga ccacctcgcc
5401 ttacacatga agaggcattt tGGATCTGGA GCAACAAACT TCTCACTACT CAAACAAGCA
5461 GGTGACGTGG AGGAGAATCC CGGCCCTatg cccctcaacg ttagcttcac caacaggaac
5521 tatgacctcg actacgactc ggtgcagccg tatttctact gcgacgagga ggagaacttc
5581 taccagcagc agcagcagag cgagctgcag cccccggcgc ccagcgagga tatctggaag
5641 aaattcgagc tgctgcccac cccgcccctg tcccctagcc gccgctccgg gctctgctcg
5701 ccctcctacg ttgcggtcac acccttctcc cttcggggag acaacgacgg cggtggcggg
5761 agcttctcca cggccgacca gctggagatg gtgaccgagc tgctgggagg agacatggtg
5821 aaccagagtt tcatctgcga cccggacgac gagaccttca tcaaaaacat catcatccag
5881 gactgtatgt ggagcggctt ctcggccgcc gccaagctcg tctcagagaa gctggcctcc
5941 taccaggctg cgcgcaaaga cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc
6001 tccacctcca gcttgtacct gcaggatctg agcgccgccg cctcagagtg catcgacccc
6061 tcggtggtct tcccctaccc tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa
6121 gactccagcg ccttctctcc gtcctcggat tctctgctct cctcgacgga gtcctccccg
6181 cagggcagcc ccgagcccct ggtgctccat gaggagacac cgcccaccac cagcagcgac
6241 tctgaggagg aacaagaaga tgaggaagaa atcgatgttg tttctgtgga aaagaggcag
6301 gctcctggca aaaggtcaga gtctggatca ccttctgctg gaggccacag caaacctcct
6361 cacagcccac tggtcctcaa gaggtgccac gtctccacac atcagcacaa ctacgcagcg
6421 cctccctcca ctcggaagga ctatcctgct gccaagaggg tcaagttgga cagtgtcaga
6481 gtcctgagac agatcagcaa caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag
6541 gagaatgtca agaggcgaac acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa
6601 cggagctttt ttgccctgcg tgaccagatc ccggagttgg aaaacaatga aaaggccccc
6661 aaggtagtta tccttaaaaa agccacagca tacatcctgt ccgtccaagc agaggagcaa
6721 aagctcattt ctgaagagga cttgttgcgg aaacgacgag aacagttgaa acacaaactt
6781 gaacagctac ggaactcttg tgcgGGATCT GGACAATGTA CTAACTACGC TTTGTTGAAA
6841 CTCGCTGGCG ATGTTGAAAG TAACCCCGGT CCCatgtaca acatgatgga gacggagctg
6901 aagccgccgg gcccgcagca aacttcgggg ggcggcggcg gcaactccac cgcggcggcg
6961 gccggcggca accagaaaaa cagcccggac cgcgtcaagc ggcccatgaa tgccttcatg
7021 gtgtggtccc gcgggcagcg gcgcaagatg gcccaggaga accccaagat gcacaactcg
7081 gagatcagca agcgcctggg cgccgagtgg aaacttttgt cggagacgga gaagcggccg
7141 ttcatcgacg aggctaagcg gctgcgagcg ctgcacatga aggagcaccc ggattataaa
7201 taccggcccc ggcggaaaac caagacgctc atgaagaagg ataagtacac gctgcccggc
7261 gggctgctgg cccccggcgg caatagcatg gcgagcgggg tcggggtggg cgccggcctg
7321 ggcgcgggcg tgaaccagcg catggacagt tacgcgcaca tgaacggctg gagcaacggc
7381 agctacagca tgatgcagga ccagctgggc tacccgcagc acccgggcct caatgcgcac
7441 ggcgcagcgc agatgcagcc catgcaccgc tacgacgtga gcgccctgca gtacaactcc
7501 atgaccagct cgcagaccta catgaacggc tcgcccacct acagcatgtc ctactcgcag
7561 cagggcaccc ctggcatggc tcttggctcc atgggttcgg tggtcaagtc cgaggccagc
7621 tccagccccc ctgtggttac ctcttcctcc cactccaggg cgccctgcca ggccggggac
7681 ctccgggaca tgatcagcat gtatctcccc ggcgccgagg tgccggaacc cgccgccccc
7741 agcagacttc acatgtccca gcactaccag agcggcccgg tgcccggcac ggccattaac
7801 ggcacactgc ccctctcaca catgtgagtc gacaagaatt cgatatcaag cttatcgata
7861 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
7921 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
7981 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
8041 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
8101 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
8161 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
8221 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
8281 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
8341 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
8401 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
8461 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
8521 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
8581 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
8641 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
8701 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
8761 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
8821 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
8881 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
8941 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
9001 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
9061 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
9121 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
9181 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
9241 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
9301 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
9361 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
9421 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
9481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
9541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
9601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
9661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
9721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
9781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
9841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
9901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
9961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
10021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
10081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
10141 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
10201 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
10261 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
10321 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
10381 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
10441 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
10501 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
10561 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
10621 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
10681 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
10741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
10801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
10861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
10921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
10981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
11041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
11101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
11161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
11221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
11281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
11341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
11401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
11461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
11521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
11581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
11641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
11701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
11761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
11821 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
11881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
11941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
12001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
12061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
12121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
12181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
12241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
12301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
12361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
12421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
12481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
12541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
12601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
12661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
12721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
12781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
12841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
12901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
12961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
13021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
13081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
13141 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
13201 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
13261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
13321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
13381 gatgtacggg ccagatata
//
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