FUW-tetO-hOKMS

价格:1100元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: FUW-tetO-hOKMS
质粒类型: 慢病毒载体;四环素调控载体;Tet-On系统载体
高拷贝/低拷贝: 高拷贝
克隆方法: EcoRI
启动子: TRE/Minimal CMV
载体大小: 13399 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: bleomycin, phleomycin, and Zeocin?
克隆菌株: Stbl3
宿主细胞(系): 大部分哺乳动物细胞
备注: FUW-tetO-hOKMS vector expressing the reverse tetracycline transactivator. Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2.
产品目录号: #51543
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

FUW-tetO-hOKMS载体图谱



载体简介

载体序列

LOCUS       FUW-tetO-hOKMS	13399 bp 	DNA	SYN
DEFINITION  FUW-tetO-hOKMS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="FUW-tetO-hOKMS"
                     /mol_type="other DNA"
     promoter        8..584
                     /label="CMV_immearly_promoter"
     misc_feature    87..374
                     /label="CAG_enhancer"
     misc_feature    541..561
                     /label="CMV_fwd_primer"
     misc_feature    604..784
                     /label="HIV-1_5_LTR"
     misc_feature    604..784
                     /label="truncHIV-1_3_LTR"
     misc_feature    895..939
                     /label="HIV-1_psi_pack"
     CDS             1327..2214
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    1449..1682
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2213..2228
                     /label="cPPT"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2371..2688
                     /label="TRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     promoter        2689..2808
                     /label="CMV2_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2720..2739
                     /label="pCEP_fwd_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2722..2746
                     /label="LNCX_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     CDS             2869..7827
                     /label="ORF frame 1"
                     /translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGG
                     PGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVG
                     VESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITL
                     GYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQ
                     EICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRV
                     WFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALY
                     SSVPFPEGEAFPPVSVTTLGSPMHSNGSGEGRGSLLTCGDVEENPGPMAVSDALLPSF
                     STFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGG
                     AGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSP
                     SSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVS
                     PSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSK
                     GSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQ
                     LPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQEL
                     MPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPY
                     HCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFGSGAT
                     NFSLLKQAGDVEENPGPMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQ
                     PPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQL
                     EMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARK
                     DSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSA
                     FSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAP
                     GKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVR
                     VLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEK
                     APKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCAGSGQCTNY
                     ALLKLAGDVESNPGPMYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKR
                     PMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALH
                     MKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDS
                     YAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYM
                     NGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMIS
                     MYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM*"
     CDS             complement(4369..5220)
                     /label="ORF frame 2"
                     /translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
                     ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
                     LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
                     FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
                     AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
                     RRRRRGG*"
     misc_feature    6715..6744
                     /label="Myc"
                     /translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
                     ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
                     LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
                     FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
                     AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
                     RRRRRGG*"
     misc_feature    7862..8449
                     /label="WPRE"
                     /translation="MGTLCVGFAAARVITSVGGGPFPGPRSSSPLWLGLLWHAGTGWH
                     ELLVMERRDLRLHLIGQEGWVIGPRVGMEAGRKRQGRVTVPAAQHFLKTQGRGSPAGE
                     LPPQGEVVCSRPVAIAERGSSAQVGARRDRLLLDLGARARRPAVVGRHHHRVAAVRAA
                     FADADDRRAAVLAARGAQRRLQHELAHQPATWRLRLLRRNVHRVQFWPQELGHEAARG
                     AHVVDVRQVEGSRRRGSGLPAIEEASARAARRHARVVPGPDRIGEAAGGGRAGRAAAR
                     RRRRRGG*"
     CDS             7950..8999
                     /label="ORF frame 3"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPHRYRRPRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMT
                     YKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLT
                     FGWCYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMAR
                     ELHPDCTGSLWLDQI*"
     CDS             7963..8475
                     /label="ORF frame 1"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    complement(8452..8468)
                     /label="pBluescriptKS_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    8636..8657
                     /label="U3PPT"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    8636..8651
                     /label="cPPT"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    8973..9153
                     /label="HIV-1_5_LTR"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    8973..9153
                     /label="truncHIV-1_3_LTR"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    complement(9179..9196)
                     /label="BGH_rev_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     terminator      9182..9409
                     /label="bGH_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     rep_origin      9472..9778
                     /label="f1_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    complement(9892..9912)
                     /label="pBABE_3_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    complement(9898..10113)
                     /label="SV40_enhancer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     promoter        9910..10178
                     /label="SV40_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     rep_origin      10077..10154
                     /label="SV40_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    10139..10158
                     /label="SV40pro_F_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     promoter        10272..10339
                     /label="EM7_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    10340..10714
                     /label="sh_ble"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     gene            10340..10711
                     /label="bleo"
                     /gene="bleo"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     terminator      10847..10966
                     /label="SV40_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    10935..10954
                     /label="EBV_rev_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     promoter        complement(11010..11028)
                     /label="M13_reverse_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     misc_feature    complement(11027..11049)
                     /label="M13_pUC_rev_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     promoter        complement(11063..11092)
                     /label="lac_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     gene            complement(12175..13035)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
     CDS             complement(12175..13035)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(13077..13105)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
   61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
  121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
  181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
  241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
  301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
  361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
  421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
  481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
  541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
  601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
  661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
  721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
  781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
  841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
  901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
  961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
 2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
 2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
 2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
 2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
 2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
 2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
 2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
 2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
 2821 attcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggTat ggcgggacac
 2881 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
 2941 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
 3001 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
 3061 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
 3121 gtgccccaag gcggcttgga gacctctcag cctgagggTg aagcaggagt cggggtggag
 3181 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
 3241 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
 3301 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
 3361 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
 3421 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
 3481 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
 3541 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
 3601 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
 3661 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
 3721 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
 3781 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
 3841 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
 3901 tttccccctg tctcTgtcac cactctgggc tctcccatgc aTtcaaacGG ATCTGGAgag
 3961 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc catggctgtc
 4021 agcgacgcgc tgctcccatc tttctccacg ttcgcgtctg gcccggcggg aagggagaag
 4081 acactgcgtc aagcaggtgc cccgaataac cgctggcggg aggagctctc ccacatgaag
 4141 cgacttcccc cagtgcttcc cggccgcccc tatgacctgg cggcggcgac cgtggccaca
 4201 gacctggaga gcggcggagc cggtgcggct tgcggcggta gcaacctggc gcccctacct
 4261 cggagagaga ccgaggagtt caacgatctc ctggacctgg actttattct ctccaattcg
 4321 ctgacccatc ctccggagtc agtggccgcc accgtgtcct cgtcagcgtc agcctcctct
 4381 tcgtcgtcgc cgtcgagcag cggccctgcc agcgcgccct ccacctgcag cttcacctat
 4441 ccgatccggg ccgggaacga cccgggcgtg gcgccgggcg gcacgggcgg aggcctcctc
 4501 tatggcaggg agtccgctcc ccctccgacg gctcccttca acctggcgga catcaacgac
 4561 gtgagcccct cgggcggctt cgtggccgag ctcctgcggc cagaattgga cccggtgtac
 4621 attccgccgc agcagccgca gccgccaggt ggcgggctga tgggcaagtt cgtgctgaag
 4681 gcgtcgctga gcgcccctgg cagcgagtac ggcagcccgt cggtcatcag cgtcagcaaa
 4741 ggcagccctg acggcagcca cccggtggtg gtggcgccct acaacggcgg gccgccgcgc
 4801 acgtgcccca agatcaagca ggaggcggtc tcttcgtgca cccacttggg cgctggaccc
 4861 cctctcagca atggccaccg gccggctgca cacgacttcc ccctggggcg gcagctcccc
 4921 agcaggacta ccccgaccct gggtcttgag gaagtgctga gcagcaggga ctgtcaccct
 4981 gccctgccgc ttcctcccgg cttccatccc cacccggggc ccaattaccc atccttcctg
 5041 cccgatcaga tgcagccgca agtcccgccg ctccattacc aagagctcat gccacccggt
 5101 tcctgcatgc cagaggagcc caagccaaag aggggaagac gatcgtggcc ccggaaaagg
 5161 accgccaccc acacttgtga ttacgcgggc tgcggcaaaa cctacacaaa gagttcccat
 5221 ctcaaggcac acctgcgaac ccacacaggt gagaaacctt accactgtga ctgggacggc
 5281 tgtggatgga aattcgcccg ctcagatgaa ctgaccaggc actaccgtaa acacacgggg
 5341 caccgcccgt tccagtgcca aaaatgcgac cgagcatttt ccaggtcgga ccacctcgcc
 5401 ttacacatga agaggcattt tGGATCTGGA GCAACAAACT TCTCACTACT CAAACAAGCA
 5461 GGTGACGTGG AGGAGAATCC CGGCCCTatg cccctcaacg ttagcttcac caacaggaac
 5521 tatgacctcg actacgactc ggtgcagccg tatttctact gcgacgagga ggagaacttc
 5581 taccagcagc agcagcagag cgagctgcag cccccggcgc ccagcgagga tatctggaag
 5641 aaattcgagc tgctgcccac cccgcccctg tcccctagcc gccgctccgg gctctgctcg
 5701 ccctcctacg ttgcggtcac acccttctcc cttcggggag acaacgacgg cggtggcggg
 5761 agcttctcca cggccgacca gctggagatg gtgaccgagc tgctgggagg agacatggtg
 5821 aaccagagtt tcatctgcga cccggacgac gagaccttca tcaaaaacat catcatccag
 5881 gactgtatgt ggagcggctt ctcggccgcc gccaagctcg tctcagagaa gctggcctcc
 5941 taccaggctg cgcgcaaaga cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc
 6001 tccacctcca gcttgtacct gcaggatctg agcgccgccg cctcagagtg catcgacccc
 6061 tcggtggtct tcccctaccc tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa
 6121 gactccagcg ccttctctcc gtcctcggat tctctgctct cctcgacgga gtcctccccg
 6181 cagggcagcc ccgagcccct ggtgctccat gaggagacac cgcccaccac cagcagcgac
 6241 tctgaggagg aacaagaaga tgaggaagaa atcgatgttg tttctgtgga aaagaggcag
 6301 gctcctggca aaaggtcaga gtctggatca ccttctgctg gaggccacag caaacctcct
 6361 cacagcccac tggtcctcaa gaggtgccac gtctccacac atcagcacaa ctacgcagcg
 6421 cctccctcca ctcggaagga ctatcctgct gccaagaggg tcaagttgga cagtgtcaga
 6481 gtcctgagac agatcagcaa caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag
 6541 gagaatgtca agaggcgaac acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa
 6601 cggagctttt ttgccctgcg tgaccagatc ccggagttgg aaaacaatga aaaggccccc
 6661 aaggtagtta tccttaaaaa agccacagca tacatcctgt ccgtccaagc agaggagcaa
 6721 aagctcattt ctgaagagga cttgttgcgg aaacgacgag aacagttgaa acacaaactt
 6781 gaacagctac ggaactcttg tgcgGGATCT GGACAATGTA CTAACTACGC TTTGTTGAAA
 6841 CTCGCTGGCG ATGTTGAAAG TAACCCCGGT CCCatgtaca acatgatgga gacggagctg
 6901 aagccgccgg gcccgcagca aacttcgggg ggcggcggcg gcaactccac cgcggcggcg
 6961 gccggcggca accagaaaaa cagcccggac cgcgtcaagc ggcccatgaa tgccttcatg
 7021 gtgtggtccc gcgggcagcg gcgcaagatg gcccaggaga accccaagat gcacaactcg
 7081 gagatcagca agcgcctggg cgccgagtgg aaacttttgt cggagacgga gaagcggccg
 7141 ttcatcgacg aggctaagcg gctgcgagcg ctgcacatga aggagcaccc ggattataaa
 7201 taccggcccc ggcggaaaac caagacgctc atgaagaagg ataagtacac gctgcccggc
 7261 gggctgctgg cccccggcgg caatagcatg gcgagcgggg tcggggtggg cgccggcctg
 7321 ggcgcgggcg tgaaccagcg catggacagt tacgcgcaca tgaacggctg gagcaacggc
 7381 agctacagca tgatgcagga ccagctgggc tacccgcagc acccgggcct caatgcgcac
 7441 ggcgcagcgc agatgcagcc catgcaccgc tacgacgtga gcgccctgca gtacaactcc
 7501 atgaccagct cgcagaccta catgaacggc tcgcccacct acagcatgtc ctactcgcag
 7561 cagggcaccc ctggcatggc tcttggctcc atgggttcgg tggtcaagtc cgaggccagc
 7621 tccagccccc ctgtggttac ctcttcctcc cactccaggg cgccctgcca ggccggggac
 7681 ctccgggaca tgatcagcat gtatctcccc ggcgccgagg tgccggaacc cgccgccccc
 7741 agcagacttc acatgtccca gcactaccag agcggcccgg tgcccggcac ggccattaac
 7801 ggcacactgc ccctctcaca catgtgagtc gacaagaatt cgatatcaag cttatcgata
 7861 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
 7921 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
 7981 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
 8041 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
 8101 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
 8161 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
 8221 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
 8281 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
 8341 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
 8401 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
 8461 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
 8521 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
 8581 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
 8641 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
 8701 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
 8761 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
 8821 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
 8881 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
 8941 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
 9001 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
 9061 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
 9121 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
 9181 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
 9241 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
 9301 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
 9361 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
 9421 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
 9481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
 9541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
 9601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
 9661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
 9721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
 9781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
 9841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
 9901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
 9961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
10021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
10081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
10141 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
10201 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
10261 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
10321 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
10381 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
10441 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
10501 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
10561 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
10621 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
10681 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
10741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
10801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
10861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
10921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
10981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
11041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
11101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
11161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
11221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
11281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
11341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
11401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
11461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
11521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
11581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
11641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
11701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
11761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
11821 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
11881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
11941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
12001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
12061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
12121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
12181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
12241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
12301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
12361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
12421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
12481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
12541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
12601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
12661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
12721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
12781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
12841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
12901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
12961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
13021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
13081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
13141 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
13201 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
13261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
13321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
13381 gatgtacggg ccagatata
//

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