pSilencer5.1-U6 Retro

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen/ Ambion
载体名称: pSilencer5.1-U6 Retro, pSilencer 5.1-U6 Retro, pSilencer5.1-U6-Retro
质粒类型: 哺乳动物细胞逆病毒基因沉默载体
高拷贝/低拷贝: 低拷贝
启动子: U6
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6486bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素(Puromycin)
备注: 逆病毒基因沉默载体,能够将发卡的siRNA转染进入大多数哺乳细胞中。
产品目录号: AM5784
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pSilencer5.1-U6 Retro载体图谱



pSilencer5.1-U6 Retro多克隆位点

载体简介

载体序列

LOCUS       pSilencer\5.1-U6 Retro       6486 bp    DNA     circular      6-OCT-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pSilencer 5.1-U6 Retro|
BASE COUNT     1460 a      1840 c      1693 g      1493 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc 
      421 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 
      481 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 
      541 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt 
      601 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca 
      661 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca 
      721 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc 
      781 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga 
      841 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 
      901 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga 
      961 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac 
     1021 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa 
     1081 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac 
     1141 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg 
     1201 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca 
     1261 ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc 
     1321 cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt 
     1381 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac 
     1441 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg 
     1501 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt 
     1561 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc 
     1621 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg 
     1681 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc 
     1741 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt 
     1801 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt 
     1861 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt 
     1921 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc 
     1981 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc 
     2041 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc 
     2101 ctccctttat ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg 
     2161 tcgacggtat cgataagctt ttccaaaaaa gtactgctta cgatacggtc tcttgaaccg 
     2221 tatcgtaagc agtactggat cccgcgtcct ttccacaaga tatataaacc caagaaatcg 
     2281 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact 
     2341 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg 
     2401 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat 
     2461 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg 
     2521 gtcacgctcc gtcacgtggt gcgttttgcc tgcgcgtctt tccactgggg aattctaccg 
     2581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 
     2641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 
     2701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 
     2761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 
     2821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 
     2881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 
     2941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 
     3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 
     3061 ctcatctccg ggcctttcga cctgcagccc aagctagctt accatgaccg agtacaagcc 
     3121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 
     3181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 
     3241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 
     3301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 
     3361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 
     3421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 
     3481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 
     3541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 
     3601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 
     3661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc 
     3721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt 
     3781 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa 
     3841 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact 
     3901 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa 
     3961 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc 
     4021 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg 
     4081 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc 
     4141 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa 
     4201 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc 
     4261 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac 
     4321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     4381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     4441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     4501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     5101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     5221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     5341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     6181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     6241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     6301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     6361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     6421 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 
     6481 ttcgtc 
//

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