| 质粒名称: | Flag-SENP1m |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17358 |
| 存储实验室: | Edward Yeh |
|
载体骨架基本信息 |
|
| 载体名称: | pFlag-CMV |
| 载体出品公司: | Sigma |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SENP1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1930 bp |
| 基因突变: | R630L, K631M |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Flag（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Flag-SENP1m was a gift from Edward Yeh (Addgene plasmid # 17358 ; http://n2t.net/addgene:17358 ; RRID:Addgene_17358) |
| 参考文献部分: | SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cheng J, Kang X, Zhang S, Yeh ET. Cell. 2007 Nov 2. 131(3):584-95. 10.1016/j.cell.2007.08.045 PubMed 17981124 |
买家导航
FlagSENP1m载体基本信息
FlagSENP1m载体图谱质粒图谱和多克隆位点信息
FlagSENP1m载体简介
FlagSENP1m载体序列
LOCUS Flag-SENP1m 6605 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-SENP1m
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6605)
AUTHORS Cheng J, Kang X, Zhang S, Yeh ET
TITLE SUMO-specific protease 1 is essential for stabilization of HIF1alpha
during hypoxia.
JOURNAL Cell. 2007 Nov 2. 131(3):584-95.
PUBMED 17981124
REFERENCE 2 (bases 1 to 6605)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6605
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 113..132
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 366..383
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 500..521
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 536..566
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 574..590
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 579..601
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 598..614
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 598..614
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 628..647
/label=T7
/note="T7 promoter, forward primer"
promoter 628..646
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(685..1014)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 699..834
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(753..772)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 999..1019
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
polyA_signal 1043..1665
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind 1447..1466
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
CDS complement(3653..3676)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(3710..3913)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(3739..3759)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 3914..4293
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(4454..4471)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4454..4470)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4463..4485)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(4611..5066)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4748..4769)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4960..4979
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 5093..5197
/gene="bla"
/label=AmpR promoter
CDS 5198..6058
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5416..5435)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6229..212
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
61 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
601 gaaacagcta tgacatgatt acgaatttaa tacgactcac tatagggaat taactttctg
661 gtttttcagt tcctcgagga gctttttgca aaagcctagg cctccaaaaa agcctcctca
721 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
781 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
841 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
901 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
961 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
1021 cagccaagct ggccgcgtac ccaattcaac aggcatctac tgagtggacc caacgcatga
1081 gaggacagtg ccaagcaagc aactcaaatg tcccaccggt tgggcatggc caggtagcct
1141 atgctgtgtc tggacgtcct cctgctggta tagttatttt aaaatcagaa ggacagggaa
1201 gggagcagtg gttcacgcct gtaatcccag caatttggga ggccaaggtg ggtagatcac
1261 ctgagattag gagttggaga ccagcctggc caatatggtg aaaccccgtc tctaccaaaa
1321 aaacaaaaat tagctgagcc tggtcatgca tgcctggaat cccaacaact cgggaggctg
1381 aggcaggaga atcgcttgaa cccaggaggc ggagattgca gtgagccaag attgtgccac
1441 tgcactccag cttggttccc aatagacccc gcaggcccta caggttgtct tcccaacttg
1501 ccccttgctc cataccaccc ccctccaccc cataatatta tagaaggaca cctagtcaga
1561 caaaatgatg caacttaatt ttattaggac aaggctggtg ggcactggag tggcaacttc
1621 cagggccagg agaggcactg gggaggggtc acagggatgc cacccgggat cctctagagt
1681 cgactcatca caagagtttt cggtggagga tctcccagac catccgcatc aggaagtatg
1741 gcatgtgttg ctgtgcgaag ttgattggtc tgtctttggt aatacagtca gcatatttgc
1801 aggcaaacat cccacagtca cttccattca tctgctgagg aatctcctgg cttttcttgc
1861 tgaaaagctg ccagccattg gtgtcaaact ctttcctttt cttgtcaatg ctttcttgct
1921 ttaggtattg caagagtatt ctgcaggctt cattgtttat cccacccatg gagtcgtaat
1981 aggtaatatt cttctttcta aagtccacaa cagctagaca ccagtgtact cccaggtgaa
2041 tgggcaccaa aagaatgtca acagaaaata catctacttt ctttgtccaa cgtttcactg
2101 cctgataacc agccgttttt aatttagtga agaaaaaggt attaaatgca tgcacacttg
2161 gcaagccctt ctctttactt cgctccatca gcatattcat gtagaaattg atgatctcat
2221 cattgagcca attcagatgg tttagagttt gaatatcttt gcgtgtaatg gtcaggcgaa
2281 atgcttcact gagaacttca tcctgattcc cattacgaaa tacattcttt atttctttct
2341 ccatttcctc tgtaatttca ggaaattcat cttcactatc agttaattta tgaccttttt
2401 tttgtgtttc ttggacaaca gtaacaggaa tctccttttc aagaggtaca cgaagatgta
2461 gttctactga atcatgtact gaatgttccc gctcctgcaa tctctggttt tgaagctgta
2521 aggccaatgc cttctgttct tcaatctggc gcaatctttc tcgtgctcga gaatcataaa
2581 cactagttaa ttctttgatc cacagctctg cctggaagaa agtagaactg ggagttggag
2641 tctgggaatc tttcactttc agtaaaatca cagagtctga tccttcagat tgtgtatttg
2701 aagctgctaa gttatctggc tgatgtggaa cagagtggtg atgatgggga tgatgaagag
2761 taccagaatc tttggatcca aatgcaacat ctggggtata agaagatagg gaatatacac
2821 tgtgggacag ctgttcctgg ttagttaaaa tgctggcaga tccagatgat gaagtatcag
2881 agccaatgat ctgagatgca caagagcttc catttttaaa cagtgagtct ttcaaagtat
2941 ttttactgga actaagacat cgagacaggt gtaaaggaaa atgtgtggtg ggtttggcta
3001 tagcaaactg tttccctgtg accatctgta gcagctgtct gtaaaactct ctttcttctt
3061 cttgaactgt ctcttctgct gtactaacat gtcgcctctg agttttcttg gggctcaaaa
3121 gacttcgacg acatgaacca ctccaagatg gacttggaac aggtttaata ggaaaagatt
3181 tttcatatgc agatacatgg cagtgatggt ttgactttcc cgcaaaactg tttgataatc
3241 cacttgaggt ctttcgggtt tcgaggtaaa gacttcggct gtttcttgat ttttgtaaag
3301 atgagcttga cgatggggta gaatttctcc attggccatt tgcactctgg ccaaaggttc
3361 ttaaatcgcc tgagccaaga aaactgtctg aggaaggatt atctgagtaa tagcttggat
3421 tataagctgc acttcttgtg gaacatgtaa aagatcggtc caaatgtcct tgcctggaag
3481 ataaaatctg ctggtcagaa agcgaaagct ggtcctctgg aaaacctgtt tgtggcagga
3541 ggtgggtttt gaatacggag ttgtggttca ctaaagtcac ttctccagca tccatcctca
3601 tcctatcagc aatatcatcc ataagatcta tcgatgaatt cgcggccgca agcttgtcgt
3661 catcgtcttt gtagtccatg gtagatcaat tctgacggtt cactaaacga gctctgctta
3721 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caacggggcg gggttattac
3781 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
3841 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg gtgtactgcc
3901 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
3961 agtcccgtaa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
4021 caataggggg cggacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
4081 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
4141 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
4201 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
4261 attactatta ataactagtc aataatcaat gtcaacatgg cggtcatatt ggacatgagc
4321 caatataaat gtacatatta tgatatagat acaacgtatg caatggccaa tagccaatat
4381 tgatttatgc tatataacca atgactaata tggctaattg ccaatattga ttcaatgtat
4441 agatcagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
4501 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
4561 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
4621 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
4681 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
4741 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
4801 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
4861 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
4921 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
4981 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
5041 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
5341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
6061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
6121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
6361 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
6601 gagat
//
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