| 质粒名称: | pRJPaph-bjGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 67032 |
| 存储实验室: | Julia Vorholt |
|
载体骨架基本信息 |
|
| 载体名称: | pSUP202pol4 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 结合细菌载体 |
| 筛选标记: | -- |
| 载体抗性: | 四环素,10 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | B. diazoefficiens DNA from scoI downstream region (comprising blr1132') |
| 物种来源: | Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110) |
| 插入大小: | 807 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRJPaph-bjGFP was a gift from Julia Vorholt (Addgene plasmid # 67032 ; http://n2t.net/addgene:67032 ; RRID:Addgene_67032) |
| 参考文献部分: | Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoefficiens to analyze host-plant infection and colonization. Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM. Mol Plant Microbe Interact. 2015 Jun 2. 10.1094/MPMI-03-15-0054-TA PubMed 26035130 |
买家导航
pRJPaphbjGFP载体基本信息
pRJPaphbjGFP载体图谱质粒图谱和多克隆位点信息
pRJPaphbjGFP载体简介
pRJPaphbjGFP载体序列
LOCUS pRJPaph-bjGFP 8710 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens
(B. japonicum) scoI.
ACCESSION .
VERSION .
KEYWORDS pRJPaph-bjGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8710)
AUTHORS Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
TITLE Stable fluorescent and enzymatic tagging of Bradyrhizobium
diazoefficiens to analyze host-plant infection and colonization.
JOURNAL Mol Plant Microbe Interact. 2015 Jun 2.
PUBMED 26035130
REFERENCE 2 (bases 1 to 8710)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8710
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 57..1247
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
CDS 1888..2079
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(2073..2095)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 2181..2321
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 2236..2255
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(2336..2353)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2507..3095)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2587..2606)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4951..4970
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5210..5231
/label=EGFP-N
/note="EGFP, reverse primer"
CDS complement(6927..7298)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 7331..7440
/note="incP origin of transfer"
promoter 8691..9
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
ORIGIN
1 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
61 aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
121 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
181 ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
241 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
301 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
601 aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
661 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
721 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
781 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
841 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
901 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
961 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
1021 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
1081 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
1141 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
1201 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
1261 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
1321 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
1381 ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
1441 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
1501 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
1561 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
1621 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
1681 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
1741 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
1801 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
1861 aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg
1921 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
1981 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
2041 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
2101 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
2161 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
2221 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
2281 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
2341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
2401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
2461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
2581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
2641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
2701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
2761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
2821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
2881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
2941 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
3001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
3181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
3241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
3421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc cgcggttcgg tgatgccacg
3601 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
3661 ggcgtggtcc gcccgagggc agagccatga ctttttccac cgcggtggcg gccgctctag
3721 acggtaatag ggatcggaga agtcgacgcg ggcccggagc tgcgggctca ccgccatcga
3781 ggcgatgata gcatcgcccc ggttcgaggt gagcgcgtcg accagggtct cgaagcggcg
3841 catctgcacc gtgcaggtga ccttgatctc ctcgcagagc gcgcgggcga gatcgacgtt
3901 gaagccggcc ggattgccgt cggcgccggt gtagttgaac ggcggatagt cggtctcggt
3961 caaaaagcgg atcacggtca ggcgcgacag gtccggccgc tccggccgcc gccgcgggtc
4021 ccagaagccg ggcacggcct gcggggccgc ctggggcgcg acctgcgggg tgacctgggg
4081 cgccgccgga ggctgcttgg ccggggcctg cgcctgcgcg ccgggcaagc cggcgaacag
4141 gcaggcgccg acggccagcc ctgtcagcaa gccacgggca gatttggacg atttcgcctg
4201 ttgcgatgga gccatcggcc ggaaatgaac gaatttcgtt gggatcggag ggccttatag
4261 accatccgcc cgggaacgcg agcgccgatt gcggtcaggg tgaaggtgcg ctccctctcc
4321 cgcttgcggg aggggggcgg ggagagggtg tctccgcgga gggacaatcc ccgagaggag
4381 agagccctca cccggcgctc tgccatagcc gaggctttgc ctcggcgtct tagaggacgg
4441 ccgccaaaag cggcctatac ctctcccgca agcgggagag gttacaccga ggacgcggtc
4501 cgaccgatcc accaaactac agataacgcc tgcaagacgt cggtacccaa ttgtcatcac
4561 ttgtacagct cgtccatgcc gtgggtgatg ccggcggcgg tgacgaactc cagcaggacc
4621 atgtggtcgc gcttctcgtt cgggtccttc gacagggccg actgggtcga caggtagtgg
4681 ttgtccggca gcaggaccgg gccgtcgccg atcggggtgt tctgctggta gtggtcggcc
4741 agctggaccg agccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
4801 ttctgcttgt cggcggtgat gtagacgttg tgcgagttgt agttgtactc cagcttgtgg
4861 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttgaccagg
4921 gtgtcgccct cgaacttgac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaacgag
4981 atggtgcgct cctggacgta gccctccggc atggccgact tgaagaagtc gtggcgcttc
5041 atgtggtccg ggtagcgcga gaagcactgg acgccgtagg tcagggtggt gaccagggtc
5101 ggccacggga ccggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
5161 gtggcgtcgc cctcgccctc gcccgagacc gagaacttgt ggccgttgac gtcgccgtcc
5221 agctcgacca ggatcgggac gacgccggtg aacagctcct cgcccttcga cataattata
5281 tctccttctt atttctagtc aagatcttga tcccctgcgc catcagatcc ttggcggcaa
5341 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
5401 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
5461 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
5521 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
5581 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gcgagctcga attccgtatg
5641 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
5701 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
5761 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
5821 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
5881 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
5941 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
6001 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
6061 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac
6121 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg
6181 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
6241 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc
6301 aggctctccc cgtggaggta ataattgacg atatgatcca gccgaccagg ctttccacgc
6361 ccgcgtgccg ctccatgtcg ttcgcgcggt tctcggaaac gcgctgccgc gtttcgtgat
6421 tgtcacgctc aagcccgtag tcccgttcga gcgtcgcgca gaggtcagcg agggcgcggt
6481 aggcccgata cggctcatgg atggtgtttc gggtcgggtg aatcttgttg atggcgatat
6541 ggatgtgcag gttgtcggtg tcgtgatgca cggcactgac gcgctgatgc tcggcgaagc
6601 caagcccagc gcagatgcgg tcctcaatcg cgcgcaacgt ctccgcgtcg ggcttctctc
6661 ccgcgcggaa gctaaccagc aggtgatagg tcttgtcggc ctcggaacgg gtgttgccgt
6721 gctgggtcgc catcacctcg gccatgacag cgggcagggt gtttgcctcg cagttcgtga
6781 cgcgcacgtg acccaggcgc tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct
6841 ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc
6901 cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc
6961 atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg
7021 gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc
7081 aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg
7141 cccgtgattt tgtagccctg gccgacggcc agcaggtagg ccgacaggct catgccggcc
7201 gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt
7261 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
7321 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
7381 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
7441 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
7501 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
7561 tatgcagcgg aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca
7621 ggcaaatgca ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac
7681 accgacgagc tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag
7741 gctgcggttg cgttcctggc ggtgagggcg gatgtcgagg cggcgttagc gtccggctat
7801 gcgctcgtca ccatttggga gcacatgcgg gaaacgggga aggtcaagtt ctcctacgag
7861 acgttccgct cgcacgccag gcggcacatc aaggccaagc ccgccgatgt gcccgcaccg
7921 caggccaagg ctgcggaacc cgcgccggca cccaagacgc cggagccacg gcggccgaag
7981 caggggggca aggctgaaaa gccggccccc gctgcggccc cgaccggctt caccttcaac
8041 ccaacaccgg acaaaaagga tctactgtaa tggcgaaaat tcacatggtt ttgcagggca
8101 agggcggggt cggcaagtcg gccatcgccg cgatcattgc gcagtacaag atggacaagg
8161 ggcagacacc cttgtgcatc gacaccgacc cggtgaacgc gacgttcgag ggctacaagg
8221 ccctgaacgt ccgccggctg aacatcatgg ccggcgacga aattaactcg cgcaacttcg
8281 acaccctggt cgagctgatt gcgccgacca aggatgacgt ggtgatcctc tacgccggac
8341 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
8401 tcaccgatgg ggaagatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
8461 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
8521 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
8581 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
8641 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccatgt ttgacagctt
8701 atcatcgata
//
