| 质粒名称: | CRY2olig-BFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 155355 |
| 存储实验室: | Xiaowei Zhuang |
|
载体骨架基本信息 |
|
| 载体名称: | pFUGW |
| 载体类型: | 慢病毒载体 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | cry2olig-BFP |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | UBC: GTGAGGCGTCAGTTTCTTTG |
| 3’测序引物 | CGGAAAGGAGCTGACAGGTG |
|
文献引用方法 |
|
| 材料和方法部分: | CRY2olig-BFP was a gift from Xiaowei Zhuang (Addgene plasmid # 155355 ; http://n2t.net/addgene:155355 ; RRID:Addgene_155355) |
| 参考文献部分: | m(6)A-binding YTHDF proteins promote stress granule formation. Fu Y, Zhuang X. Nat Chem Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 10.1038/s41589-020-0524-y. 10.1038/s41589-020-0524-y PubMed 32451507 |
买家导航
CRY2oligBFP载体基本信息
CRY2oligBFP载体图谱质粒图谱和多克隆位点信息
CRY2oligBFP载体简介
CRY2oligBFP载体序列
LOCUS CRY2olig-BFP 11496 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Lentivector to express CRY2olig-BFP control.
ACCESSION .
VERSION .
KEYWORDS CRY2olig-BFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11496)
AUTHORS Fu Y, Zhuang X
TITLE m(6)A-binding YTHDF proteins promote stress granule formation.
JOURNAL Nat Chem Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi:
10.1038/s41589-020-0524-y.
PUBMED 32451507
REFERENCE 2 (bases 1 to 11496)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11496
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2203..2320
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2385..3596
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3433..3452
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3610..5103
/codon_start=1
/product="N-terminal photolyase homology region of
Arabidopsis thaliana cryptochrome 2"
/label=CRY2PHR
/note="binds to CIB1 upon exposure to blue light (Kennedy
et al., 2010)"
/translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
CDS 5176..5871
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
LPSKLGHKLN"
misc_feature 5951..6539
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6004..6024)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6422..6433)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6542..6558)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6543..6559)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7064..7244
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7270..7287)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7276..7500
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7546..7974
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7633..7652)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7843..7864
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7983..8003)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7988..8317
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8168..8303
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8230..8249
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8365..8412
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8431..8805
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8935..9056
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8972..8991)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9026..9045
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9105..9121)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9105..9121)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9118..9140)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9129..9145
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9153..9183)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9198..9219
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9336..9353)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9507..10095)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9587..9606)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10266..11126)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10889..10908
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11127..11231)
/gene="bla"
/label=AmpR promoter
primer_bind complement(11306..11325)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2341 cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt
2401 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
2461 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
2521 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
2581 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
2641 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
2701 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
2761 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
2821 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
2881 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
2941 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
3001 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
3061 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
3121 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
3181 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
3241 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
3301 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
3361 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
3421 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
3481 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
3541 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacagga
3601 tccgccacca tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
3661 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
3721 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
3781 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
3841 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
3901 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
3961 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
4021 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
4081 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
4141 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
4201 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
4261 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
4321 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
4381 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
4441 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
4501 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
4561 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
4621 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
4681 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
4741 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
4801 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
4861 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
4921 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
4981 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
5041 cgtgagctac tagctaaagc tatttcaaga acccgtggag cacagatcat gatcggagca
5101 gcagcccggg atccaccggt cgccaccgat ccaggtggtg gttcaggagg cggcggaggt
5161 ggaggtggaa gcggtagcga gctgattaag gagaacatgc acatgaagct gtacatggag
5221 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
5281 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
5341 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
5401 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
5461 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
5521 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
5581 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
5641 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
5701 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
5761 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
5821 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tggtggaggt
5881 agtggaagtg gcggaagcgg aagcctaggc gcgccacctg caggttgaat tcgatatcaa
5941 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
6001 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
6061 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
6121 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
6181 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
6241 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
6301 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
6361 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
6421 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
6481 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
6541 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
6601 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
6661 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
6721 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
6781 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
6841 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
6901 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
6961 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
7021 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
7081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
7141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
7201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
7261 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
7321 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
7381 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
7441 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
7501 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
7561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
7621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
7681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
7741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
7801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
7861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
7921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
7981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
8041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
8101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
8161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
8221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
8281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
8341 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
8401 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
8461 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
8521 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
8581 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
8641 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
8701 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
8761 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
8821 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
8881 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
8941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
9001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
9061 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
9121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
9181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
9241 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
9301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
9361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
9421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
9481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
9541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
9601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
9661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
9721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
9781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
9841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
9901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
9961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
10021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
10081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
10141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
10201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
10261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
10321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
10381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
10441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
10501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
10561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
10621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
10681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
10741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
10801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
10861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
10921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
10981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
11041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
11101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
11161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
11221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
11281 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
11341 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
11401 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
11461 cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
