| 质粒名称: | pCS-PesaRlux |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 47640 |
| 存储实验室: | Cynthia Collins |
|
载体骨架基本信息 |
|
| 载体名称: | pCS26 |
| 载体出品公司: | M. Surette (McMaster University) |
| 空载体大小: | 9360bp |
| 完整质粒大小: | 9646 bp |
| 载体类型: | Bacterial Expression, Synthetic Biology |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PesaR / esaR promoter |
| 物种来源: | Pantoea stewartii subsp. stewartii |
| 插入大小: | 286 bp |
| 启动子： | PesaR |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XhoI(未破坏) |
| 3’克隆位点: | BamHI (未破坏) |
| 5’测序引物 | CCAGCTGGCAATTCCGA |
| 3’测序引物 | AATCATCACTTTCGGGAA |
|
文献引用方法 |
|
| 材料和方法部分: | pCS-PesaRlux was a gift from Cynthia Collins (Addgene plasmid # 47640 ; http://n2t.net/addgene:47640 ; RRID:Addgene_47640) |
| 参考文献部分: | Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. Shong J, Huang YM, Bystroff C, Collins CH. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. 10.1021/cb3006402 PubMed 23363022 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCS-PesaRlux 9646 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION wt esaR promoter in pCS26.
ACCESSION .
VERSION .
KEYWORDS pCS-PesaRlux
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9646)
AUTHORS Shong J, Huang YM, Bystroff C, Collins CH
TITLE Directed evolution of the quorum-sensing regulator EsaR for
increased signal sensitivity.
JOURNAL ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
2013 Feb 6.
PUBMED 23363022
REFERENCE 2 (bases 1 to 9646)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9646
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 312..1754
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 1766..2689
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 2738..3820
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 3835..4818
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
CDS 4997..6109
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
terminator 6142..6228
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 6341..7291
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
terminator 8510..8604
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(8635..9429)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8746..8765)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 9356..9375
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9608..9626
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gcagattgag taaccgtgaa tgtttgtaca aatgtttcaa agatgttact atgagtgtcc
61 cggccagcat cactttatat tttgtgacgt ctggccggac gttttcccta gtgttggctg
121 ttttagcgac ctggccgtac aggtcaggtt tttttttacc gctaaacaac tgaagccatt
181 gtaacctctg aatgattcat tgtaagttac tcttaagtat catcttgcct gtactatagt
241 gcaggttaag tccacgttaa gtaaaagaag cagcggatcc tctagttgcg gccgcaaaat
301 ggatggcaaa tatgactaaa aaaatttcat tcattattaa cggccaggtt gaaatctttc
361 ccgaaagtga tgatttagtg caatccatta attttggtga taatagtgtt tacctgccaa
421 tattgaatga ctctcatgta aaaaacatta ttgattgtaa tggaaataac gaattacggt
481 tgcataacat tgtcaatttt ctctatacgg tagggcaaag atggaaaaat gaagaatact
541 caagacgcag gacatacatt cgtgacttaa aaaaatatat gggatattca gaagaaatgg
601 ctaagctaga ggccaattgg atatctatga ttttatgttc taaaggcggc ctttatgatg
661 ttgtagaaaa tgaacttggt tctcgccata tcatggatga atggctacct caggatgaaa
721 gttatgttcg ggcttttccg aaaggtaaat ctgtacatct gttggcaggt aatgttccat
781 tatctgggat catgtctata ttacgcgcaa ttttaactaa gaatcagtgt attataaaaa
841 catcgtcaac cgatcctttt accgctaatg cattagcgtt aagttttatt gatgtagacc
901 ctaatcatcc gataacgcgc tctttatctg ttatatattg gccccaccaa ggtgatacat
961 cactcgcaaa agaaattatg cgacatgcgg atgttattgt cgcttgggga gggccagatg
1021 cgattaattg ggcggtagag catgcgccat cttatgctga tgtgattaaa tttggttcta
1081 aaaagagtct ttgcattatc gataatcctg ttgatttgac gtccgcagcg acaggtgcgg
1141 ctcatgatgt ttgtttttac gatcagcgag cttgtttttc tgcccaaaac atatattaca
1201 tgggaaatca ttatgaggaa tttaagttag cgttgataga aaaacttaat ctatatgcgc
1261 atatattacc gaatgccaaa aaagattttg atgaaaaggc ggcctattct ttagttcaaa
1321 aagaaagctt gtttgctgga ttaaaagtag aggtggatat tcatcaacgt tggatgatta
1381 ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg taccttcatc
1441 acgtcgataa tattgagcaa atattgcctt atgttcaaaa aaataagacg caaaccatat
1501 ctatttttcc ttgggagtca tcatttaaat atcgagatgc gttagcatta aaaggtgcgg
1561 aaaggattgt agaagcagga atgaataaca tatttcgagt tggtggatct catgacggaa
1621 tgagaccgtt gcaacgatta gtgacatata tttctcatga aaggccatct aactatacgg
1681 ctaaggatgt tgcggttgaa atagaacaga ctcgattcct ggaagaagat aagttccttg
1741 tatttgtccc ataataggta aaagtatgga aaatgaatca aaatataaaa ccatcgacca
1801 cgttatttgt gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa
1861 cagcccaaag agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca
1921 ttttgctggt ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc
1981 gcttcaccac gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa
2041 gcagagcttg ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat
2101 gttggcttca agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc
2161 gtttttaatc accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg
2221 gtttgattat ctcagtctac ccattaatga attgccggat aatctagatt ttgaaggcca
2281 taaattgggt gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc
2341 ttctacaatt aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa
2401 cgataattgg gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg
2461 atgcaagata tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct
2521 gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga
2581 tattgatgtt gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga
2641 acgccgaatg agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag
2701 atattctatc actcaaatag caatataagg actctctatg aaatttggaa actttttgct
2761 tacataccaa cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg
2821 tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga
2881 gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa
2941 attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga
3001 agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg
3061 gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc
3121 ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa
3181 tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc
3241 accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct
3301 accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta
3361 taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata
3421 tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg
3481 gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag
3541 aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac
3601 taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat
3661 tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga
3721 agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat
3781 gccatttctt aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa
3841 tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt
3901 cgcatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat
3961 gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg
4021 ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat
4081 cctgtcgcca tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt
4141 ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa
4201 tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc
4261 tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct
4321 tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg
4381 gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat
4441 gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata
4501 gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag
4561 acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat
4621 aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg
4681 gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg
4741 aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac
4801 cacatggaat atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc
4861 aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg
4921 aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt
4981 gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga
5041 aattgatgat ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa
5101 aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata
5161 tcgtcactac tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc
5221 tgtattccca acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat
5281 tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag
5341 attaagtatt gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg
5401 gtttgatcat caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat
5461 ttggtttaaa tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac
5521 agaagaacga atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg
5581 gaaagatctt tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa
5641 agataaaaaa atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg
5701 gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac
5761 tttcaatctc agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac
5821 ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc
5881 gcttgatcct gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat
5941 ggatgcgtca gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag
6001 cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag
6061 gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt gatagttgat atcctttgcc
6121 tgcggccgca actagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
6181 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
6241 acctagcctt aataaggcct taattaaggc tagtaattat cattgactag cccatctcaa
6301 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
6361 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
6421 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
6481 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
6541 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
6601 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
6661 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
6721 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
6781 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
6841 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
6901 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
6961 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
7021 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
7081 aacaaccatt acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc
7141 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
7201 taagcatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
7261 gaacatactg gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg
7321 aagcatcaag actaacaaac aaaagtagaa caactgttca ccgttagata tcaaagggaa
7381 aactgtccat atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
7441 gagtttttgg tgcatttaaa gctgttcacc atgaacagat cgacaatgta acagatgaac
7501 agcatgtaac acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa
7561 ttgaacacct gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg
7621 cgatgctgct tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg
7681 ggaaaaaatc atggcaattc tggaagaaat agcgctttca gccggcaaac ctgaagccgg
7741 atctgcgatt ctgataacaa actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac
7801 ccgtacccct taattaaggt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
7861 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
7921 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
7981 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
8041 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
8101 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
8161 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
8221 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
8281 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
8341 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
8401 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
8461 cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa
8521 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
8581 ttactggatc tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa
8641 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
8701 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
8761 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
8821 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
8881 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
8941 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
9001 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
9061 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
9121 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
9181 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
9241 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
9301 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
9361 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
9421 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
9481 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
9541 agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca tgacattaac
9601 ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgag
//
