| 质粒名称: | pAAVLINK-EF1a-HA-5'-EP300 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001687 |
| 产品用途: | 5' AAVLINK plasmid for EP300 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EP300 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-EP300
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-EP300载体质粒基本信息
pAAVLINK-EF1a-HA-5'-EP300质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-EP300载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-EP300质粒载体简介
pAAVLINK-EF1a-HA-5'-EP300质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-EP300 7195 bp DNA circular SYN 19-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7195)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7195
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4335
/codon_start=1
/label=EP300
/translation="AENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDE
LINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMA
SQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMG
MNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPL
QQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSN
NLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQ
LVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQII
SHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQI
DPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNG
GVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQ
DLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEK
IYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIR
GSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPR
MQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQ
GSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPG
PQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPL
LPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPA
DTQPEDVSESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIF
KPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDT
GQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRK
LEFSPQTLCCYGKQLCTIPRDATYYSYQN"
misc_feature 2936..2941
/label=SDS
misc_feature 3341..3346
/label=SDS
misc_feature 3819..3824
/label=SDS
intron 4336..4376
/label=Splicing Donor
misc_feature 4380..4413
/label=lox2272-JT1571
repeat_region 4458..4598
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4458..4587
/label=AAV2 ITR
rep_origin 4673..5128
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5410..5514
/gene="bla"
/label=AmpR promoter
CDS 5515..6375
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6546..7134
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctgc cgagaatgtg gtggaaccgg ggccgccttc
781 agccaagcgg cctaaactct catctccggc cctctcggcg tccgccagcg atggcacaga
841 ttttggctct ctatttgact tggagcacga cttaccagat gaattaatca actctacaga
901 attgggacta accaatggtg gtgatattaa tcagcttcag acaagtcttg gcatggtaca
961 agatgcagct tctaaacata aacagctgtc agaattgctg cgatctggta gttcccctaa
1021 cctcaatatg ggagttggtg gcccaggtca agtcatggcc agccaggccc aacagagcag
1081 tcctggatta ggtttgataa atagcatggt caaaagccca atgacacagg caggcttgac
1141 ttctcccaac atggggatgg gcactagtgg accaaatcag ggtcctacgc agtcaacagg
1201 tatgatgaac agtccagtaa atcagcctgc catgggaatg aacacaggga tgaatgcggg
1261 catgaatcct ggaatgttgg ctgcaggcaa tggacaaggg ataatgccta atcaagtcat
1321 gaacggttca attggagcag gccgagggcg acagaatatg cagtacccaa acccaggcat
1381 gggaagtgct ggcaacttac tgactgagcc tcttcagcag ggctctcccc agatgggagg
1441 acaaacagga ttgagaggcc cccagcctct taagatggga atgatgaaca accccaatcc
1501 ttatggttca ccatatactc agaatcctgg acagcagatt ggagccagtg gccttggtct
1561 ccagattcag acaaaaactg tactatcaaa taacttatct ccatttgcta tggacaaaaa
1621 ggcagttcct ggtggaggaa tgcccaacat gggtcaacag ccagccccgc aggtccagca
1681 gccaggcctg gtgactccag ttgcccaagg gatgggttct ggagcacata cagctgatcc
1741 agagaagcgc aagctcatcc agcagcagct tgttctcctt ttgcatgctc acaagtgcca
1801 gcgccgggaa caggccaatg gggaagtgag gcagtgcaac cttccccact gtcgcacaat
1861 gaagaatgtc ctaaaccaca tgacacactg ccagtcaggc aagtcttgcc aagtggcaca
1921 ctgtgcatct tctcgacaaa tcatttcaca ctggaagaat tgtacaagac atgattgtcc
1981 tgtgtgtctc cccctcaaaa atgctggtga taagagaaat caacagccaa ttttgactgg
2041 agcacccgtt ggacttggaa atcctagctc tctaggggtg ggtcaacagt ctgcccccaa
2101 cctaagcact gttagtcaga ttgatcccag ctccatagaa agagcctatg cagctcttgg
2161 actaccctat caagtaaatc agatgccgac acaaccccag gtgcaagcaa agaaccagca
2221 gaatcagcag cctgggcagt ctccccaagg catgcggccc atgagcaaca tgagtgctag
2281 tcctatggga gtaaatggag gtgtaggagt tcaaacgccg agtcttcttt ctgactcaat
2341 gttgcattca gccataaatt ctcaaaaccc aatgatgagt gaaaatgcca gtgtgccctc
2401 cctgggtcct atgccaacag cagctcaacc atccactact ggaattcgga aacagtggca
2461 cgaagatatt actcaggatc ttcgaaatca tcttgttcac aaactcgtcc aagccatatt
2521 tcctacgccg gatcctgctg ctttaaaaga cagacggatg gaaaacctag ttgcatatgc
2581 tcggaaagtt gaaggggaca tgtatgaatc tgcaaacaat cgagcggaat actaccacct
2641 tctagctgag aaaatctata agatccagaa agaactagaa gaaaaacgaa ggaccagact
2701 acagaagcag aacatgctac caaatgctgc aggcatggtt ccagtttcca tgaatccagg
2761 gcctaacatg ggacagccgc aaccaggaat gacttctaat ggccctctac ctgacccaag
2821 tatgatccgt ggcagtgtgc caaaccagat gatgcctcga ataactccac aatctggttt
2881 gaatcaattt ggccagatga gcatggccca gccccctatt gtaccccggc aaacccctcc
2941 tcttcagcac catggacagt tggctcaacc tggagctctc aacccgccta tgggctatgg
3001 gcctcgtatg caacagcctt ccaaccaggg ccagttcctt cctcagactc agttcccatc
3061 acagggaatg aatgtaacaa atatcccttt ggctccgtcc agcggtcaag ctccagtgtc
3121 tcaagcacaa atgtctagtt cttcctgccc ggtgaactct cctataatgc ctccagggtc
3181 tcaggggagc cacattcact gtccccagct tcctcaacca gctcttcatc agaattcacc
3241 ctcgcctgta cctagtcgta cccccacccc tcaccatact cccccaagca taggggctca
3301 gcagccacca gcaacaacaa ttccagcccc tgttcctaca cctcctgcca tgccacctgg
3361 gccacagtcc caggctctac atccccctcc aaggcagaca cctacaccac caacaacaca
3421 acttccccaa caagtgcagc cttcacttcc tgctgcacct tctgctgacc agccccagca
3481 gcagcctcgc tcacagcaga gcacagcagc gtctgttcct accccaacag caccgctgct
3541 tcctccgcag cctgcaactc cactttccca gccagctgta agcattgaag gacaggtatc
3601 aaatcctcca tctactagta gcacagaagt gaattctcag gccattgctg agaagcagcc
3661 ttcccaggaa gtgaagatgg aggccaaaat ggaagtggat caaccagaac cagcagatac
3721 tcagccggag gatgtttcag agtctaaagt ggaagactgt aaaatggaat ctaccgaaac
3781 agaagagaga agcactgagt taaaaactga aataaaagag gaggaagacc agccaagtac
3841 ttcagctacc cagtcatctc cggctccagg acagtcaaag aaaaagattt tcaaaccaga
3901 agaactacga caggcactga tgccaacatt ggaggcactt taccgtcagg atccagaatc
3961 ccttcccttt cgtcaacctg tggaccctca gcttttagga atccctgatt actttgatat
4021 tgtgaagagc cccatggatc tttctaccat taagaggaag ttagacactg gacagtatca
4081 ggagccctgg cagtatgtcg atgatatttg gcttatgttc aataatgcct ggttatataa
4141 ccggaaaaca tcacgggtat acaaatactg ctccaagctc tctgaggtct ttgaacaaga
4201 aattgaccca gtgatgcaaa gccttggata ctgttgtggc agaaagttgg agttctctcc
4261 acagacactg tgttgctacg gcaaacagtt gtgcacaata cctcgtgatg ccacttatta
4321 cagttaccag aacaggtaag tatcaaggtt acaagacagg tttaaggaga ccaataagct
4381 attattcgta taggatactt tatacgaagt tattaagtta tggagaccgg tgtggcacgt
4441 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
4501 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
4561 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4621 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4741 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4861 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4981 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
5041 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5101 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5161 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5221 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5281 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5341 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
6001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
6121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
6181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
6241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
7141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
