| 质粒名称: | pOpen-9N7polA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165500 |
| 存储实验室: | Drew Endy, Jennifer Molloy, FreeGenes Project |
|
载体骨架基本信息 |
|
| 载体名称: | pBuild |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 4451 bp |
| 载体修饰: | -- |
| 载体类型: | Synthetic Biology |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 9度N-7 DNA聚合酶 |
| 物种来源: | Other |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AATG(未知是否破坏) |
| 3’克隆位点: | GCTT(未知是否破坏) |
| 5’测序引物 | M13 forward |
| 3’测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pOpen-9N7polA was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165500 ; http://n2t.net/addgene:165500 ; RRID:Addgene_165500) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pOpen-9N7polA 4451 bp ds-DNA circular SYN 26-JUL-2021
DEFINITION Thermophilic DNA polymerase; genetically engineered to have a
decreased 3'-5' proofreading exonuclease activity.
ACCESSION .
VERSION .
KEYWORDS pOpen-9N7polA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4451)
TITLE Open Enzyme Collection
REFERENCE 2 (bases 1 to 4451)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 26, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4451
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2474..2492)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2488..2535
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2540..2556)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2540..2556)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin complement(2623..3211)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2703..2722)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3402..4262)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4025..4044
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4263..4367)
/gene="bla"
/label=AmpR promoter
primer_bind 4383..4399
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 4400..4431
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 aaaggggccg tcaatatcag ggtctctaat gatcctggat actgattata tcaccgaaaa
61 cggcaaacca gttatccgtg tgttcaaaaa agagaacggt gagtttaaaa tcgaatatga
121 ccgtactttc gagccgtact tctacgctct gctgaaggat gattctgcca ttgaggatgt
181 taaaaaagta accgctaagc gccacggtac ggtggtaaag gtcaaacgtg ctgaaaaagt
241 tcagaaaaag ttcctcggtc gtccgattga agtttggaaa ctgtacttca accacccgca
301 ggacgtaccg gcaattcgtg accgtattcg cgcacacccg gcggtcgtag atatctacga
361 atatgatatt ccgtttgcta aacgctacct gatcgacaaa ggtctgatcc cgatggaagg
421 tgatgaggag ctgaccatgc tcgcgttcga tatcgaaact ctgtaccacg aaggtgaaga
481 gtttggtact ggtccgattc tgatgatttc ctacgccgac ggtagcgaag cgcgtgtgat
541 cacttggaaa aaaatcgatc tgccgtacgt tgatgtggtt tctactgaaa aagaaatgat
601 caaacgtttc ctgcgcgtgg tccgtgagaa agatccggac gtacttatca cttacaatgg
661 cgacaatttc gattttgctt acctgaaaaa acgttgcgaa gaactgggca tcaagtttac
721 cctgggtcgt gacgggagtg aacctaaaat ccagcgtatg ggcgatcgtt tcgctgtgga
781 agtgaaaggt cgcattcatt tcgatctgta cccggttatt cgtcgcacta ttaacctccc
841 gacctatact ctggaggcag tttacgaagc tgttttcggt aaaccgaaag aaaaagttta
901 tgcggaagaa attgcacaag cttgggaatc cggcgaaggt ctggaacgtg ttgcgcgcta
961 tagcatggag gacgcgaaag ttacctatga actgggtcgt gagttttttc cgatggaagc
1021 tcagctgagc cgtctgatcg gtcagtctct ttgggacgtt tctcgcagct ctaccggtaa
1081 cctcgttgaa tggtttctgc tgcgtaaggc gtacaaacgt aacgaactgg cgccgaacaa
1141 accggatgag cgtgaactgg ctcgtcgtcg tggtggctac gcaggtggct acgtgaaaga
1201 gccggaacgt ggtctgtggg acaacatcgt ttacctggat ttccgtagcc tgtacccgag
1261 tattatcatt acccacaacg taagcccgga taccctcaac cgcgaagggt gcaaagaata
1321 tgacgtcgca cctgaagttg gccacaagtt ttgtaaggac ttcccgggct tcatcccgag
1381 cctgctgggt gacctgctgg aagaacgcca gaaaatcaag cgcaaaatga aagccaccgt
1441 ggacccgctg gaaaagaaac tgctggacta ccgccagcgc gctattaaaa tcctggccaa
1501 ctctttctac ggttactatg ggtacgcaaa agcacgttgg tactgcaaag agtgcgctga
1561 atctgtgacc gcgtggggtc gtgaatatat cgagatggtt atccgtgaac ttgaagaaaa
1621 gttcggcttc aaagttctgt atgcggacac cgacggcctg catgcgacta tccctggcgc
1681 ggacgcagaa accgttaaga aaaaagcaaa agagttctta aagtacatta acccaaaact
1741 cccgggcctg ctggaactgg agtacgaggg tttctatgtt cgtggattct ttgttacgaa
1801 aaaaaaatac gcagttatcg acgaagaagg caaaatcacc acccgtggcc tggagatcgt
1861 gcgtcgtgat tggtctgaaa tcgctaaaga aacccaggcg cgcgttctgg aagcgattct
1921 gaaacacggt gacgttgaag aagccgttcg cattgtaaag gaagttacgg agaagctttc
1981 taaatacgaa gttccgccgg aaaaactggt tatccacgaa cagatcactc gtgatctgcg
2041 tgactacaag gcgaccggac cgcatgtggc ggtcgcaaaa cgcctggctg ctcgtggtgt
2101 aaaaatccgt ccgggcacgg tcatcagcta catcgtgctg aagggcagcg gccgtatcgg
2161 cgatcgtgct atcccagcgg acgagttcga cccgaccaaa caccgctatg atgctgagta
2221 ttatatcgaa aaccaggtac tgccggcggt tgaacgtatc ctgaaagcgt ttggctatcg
2281 taaagaggac ctgcgttatc agaaaaccaa acaggtaggt ctgggtgcgt ggctgaaagt
2341 caaaggcaaa aagtgaagag ctttgagacc acagaacgaa caggcactac atcaaggcat
2401 ggaacccatc atagggataa cagggtaatt gccttccttc ttaggcatcg cgctgaggtg
2461 tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
2521 ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt gatgacacac
2581 attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata ggctccgccc
2641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3181 gcagattaca cgcagaaaaa aaggatctca agaaggccta ctattagcaa caacgatcct
3241 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
3301 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
3361 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
3421 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
3481 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
3541 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
3601 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
3661 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3721 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3781 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3841 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
3901 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
3961 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
4021 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4081 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4141 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4201 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4261 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
4321 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac ctgcaccagt
4381 cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac ttggttcagg
4441 tggagtggga g
//
