LOCUS pIVT 3059 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pIVT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3059)
AUTHORS Igarashi H, Knott JG, Schultz RM, Williams CJ
TITLE Alterations of PLCbeta1 in mouse eggs change calcium oscillatory
behavior following fertilization.
JOURNAL Dev Biol. 2007 Dec 1;312(1):321-30. Epub 2007 Sep 26.
PUBMED 17961538
REFERENCE 2 (bases 1 to 3059)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3059
/organism="synthetic DNA construct"
/mol_type="other DNA"
5'UTR 8..50
/label=Xenopus globin 5'-UTR
/note="translational enhancer from the 5'-UTR of the major
beta-globin gene of Xenopus laevis"
misc_feature 57..113
/label=MCS
/note="pUC18/19 multiple cloning site"
3'UTR 151..276
/label=Xenopus globin 3'-UTR
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
primer_bind complement(337..354)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(337..353)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(346..368)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(561..580)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 680..702
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(740..758)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 826..930
/gene="bla"
/label=AmpR promoter
CDS 931..1791
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1149..1168)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1962..2550
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2451..2470
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2704..2721
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2838..2859
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2874..2904
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2912..2928
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2917..2939
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2936..2952
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2936..2952
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 2976..2994
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2976..2993
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 3006..3025
/label=T7
/note="T7 promoter, forward primer"
promoter 3006..3024
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 aagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatcaagc
61 ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgcccttc
121 tagtgactga ctaggatctg gttaccacta aaccagcctc aagaacaccc gaatggagtc
181 tctaagctac ataataccaa cttacacttt acaaaatgtt gtcccccaaa atgtagccat
241 tcgtatctgc tcctaataaa aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa
301 aaaaaaaaaa aacccccccc ccccaagggc gaattcactg gccgtcgttt tacaacgtcg
361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
421 cgctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
481 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
541 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
601 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
661 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
721 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
781 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
1201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
1501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
1741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
1801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
1861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
1921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
1981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
2521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
2581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
2641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
2701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
2761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
2821 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
2881 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
2941 aacagctatg accatgatta cgccaagctc caacgattta ggtgacacta tagaagagaa
3001 ggaattaata cgactcacta tagggagaga gagagaatta ccctcactaa agggaggag
//
