| 质粒名称: | pYPQ131-STU-Lb |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138096 |
| 存储实验室: | Yiping Qi |
|
载体骨架基本信息 |
|
| 载体名称: | pYPQ131A |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 载体抗性: | 四环素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pYPQ131-STU-Lb was a gift from Yiping Qi (Addgene plasmid # 138096 ; http://n2t.net/addgene:138096 ; RRID:Addgene_138096) |
| 参考文献部分: | Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems. Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D, Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L, Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q, Zhang Y, Qi Y. Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w. 10.1038/s41467-021-22330-w PubMed 33782402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYPQ131-STU-Lb 3181 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA
scaffold flanked by HH and HDV ribozymes.
ACCESSION .
VERSION .
KEYWORDS pYPQ131-STU-Lb
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3181)
AUTHORS Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D,
Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L,
Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q,
Zhang Y, Qi Y
TITLE Expanding the scope of plant genome engineering with Cas12a
orthologs and highly multiplexable editing systems.
JOURNAL Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w.
PUBMED 33782402
REFERENCE 2 (bases 1 to 3181)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3181
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 196..224
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 272..1462
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind complement(624..643)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(1636..1655)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1637..1655)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1660..1676)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1660..1676)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin 2030..2618
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2519..2538
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 2948..2975
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 3067..3153
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
1741 tgcctcctct agaggtctcg ctataaatta ctgatgagtc cgtgaggacg aaacgagtaa
1801 gctcgtctaa tttctactaa gtgtagatga gacggagctc agtctgaccg cggcgtctct
1861 ggccggcatg gtcccagcct cctcgctggc gccggctggt caacatgctt cggcatggcg
1921 aatgggacca tgggagaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
1981 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
2041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
2101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
2161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
2221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
2281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
2341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
2401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
2461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
2521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
2581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
2641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
2701 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
2761 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
2821 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
2881 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
2941 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
3001 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
3061 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
3121 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
3181 t
//
