| 质粒名称: | pGR475 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 213784 |
| 存储实验室: | Chang Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 11561bp |
| 载体类型: | Yeast Expression, Synthetic Biology |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Hygromycin |
| 物种来源: | S. cerevisiae (budding yeast) |
|
文献引用方法 |
|
| 材料和方法部分: | pGR475 was a gift from Chang Liu (Addgene plasmid # 213784 ; http://n2t.net/addgene:213784 ; RRID:Addgene_213784) |
买家导航
pGR475载体质粒基本信息
pGR475质粒图谱载体图谱和pGR475载体序列质粒序列多克隆位点信息
pGR475质粒载体简介
pGR475质粒序列载体序列
LOCUS pGR475 11561 bp DNA circular SYN 14-APR-2025
DEFINITION Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin
resistance marker.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11561)
TITLE Continuous evolution of user-defined genes at 1-million-times the
genomic mutation rate
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11561)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: pGR475
FEATURES Location/Qualifiers
source 1..11561
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 108..696
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 597..616
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 850..867
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 984..1005
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(1020..1025,1026..1043,1044..1050)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1058..1074
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1063..1085
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1082..1098
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1082..1098
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS complement(1658..2686)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
CDS complement(1658..2686)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
primer_bind 10439..10458
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 10533..10637
/gene="bla"
/label=AmpR promoter
CDS join(10638..10706,10707..11498)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGEPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10856..10875)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
61 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
841 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
901 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
961 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
1021 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
1081 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga cgggaattct
1141 aagctctttg ttttctaatt tagaatactc acctaactca gacattatcg gaacatgtat
1201 atctgttaga ttctggtcga gatattcttt aatggtttag tgtatgttgc gcttactact
1261 ttttggcgtt tttgcctatt tcactattta catatcgtga aaatgacatt tggttctagg
1321 ttcgggtgac gtgaagataa cgaaaaatga gtaaaaatta tcttctaatt atacattagt
1381 attagcgtga aaatgtacac atatacatat atatatatat atatatatac ttgaaggtct
1441 gaaggagttt caaatgcttc tactccgtct gctttctttt cgggagcaag attgttgtgg
1501 tgaatcatcg ataaaaataa atatactgag attatagtaa gctcattgat cccttaaact
1561 ttcttttcgg tgtatgactt atgagggtga gaatgcgaaa tggcgtggaa atgtgatcaa
1621 aggtaataaa acgtcatata ttaccctgtt atccctatta ttcctttgcc ctcggacgag
1681 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
1741 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
1801 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
1861 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
1921 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
1981 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
2041 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
2101 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
2161 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
2221 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
2281 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
2341 ccggttgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
2401 cacggcggga gatgcaatag gtcaggctct cgctaaattc cccaatgtca agcacttccg
2461 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
2521 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
2581 gagattcttc gccctccgag agctgcatca ggtcggacac gctgtcgaac ttttcgatca
2641 gaaacttctc gacagacgtc gcggtgagtt caggcttttt acccattttg ttttttgttt
2701 tcttctaatt gattttttct ttctatttcc tttggtgaaa caggtagttt tgttcaaaag
2761 aaatcattca atggtttaaa taaaagcaag aagtatgaaa atttttttat tttttcttca
2821 gaattttatc atagttgtcg taaaataacg aggtcgggta acgtgtttgg tgtactgagg
2881 tcttttttaa aagaagctcc agcaagaaag agttttgtgt gatggctaat gaagtgtcaa
2941 ttgcatttgg aaagcagcct gatttcttgg gcgtgaatat gaaattcttc ttttgttatt
3001 ttataatatg aagtatgaat gaaaacataa agcgcgctga gacaacgact gttcaatgta
3061 aaataaagaa aatctatcgt tctcaagacg tggtcccatt tttttttact ttgaatttcg
3121 tcagcagtgt gacatggtta gaatgtaacc ccacatttgc aaagagattg ttacaggagc
3181 gttctaattg tcccgtactg gtacattcat aagtgaaaag aaagccatat cgggcgcgct
3241 ggtttgtgag ccacagaaac cggacaaggc acttcttgaa tacttcccaa ggaaagcgcc
3301 aaggtttacg gttctgatgg gaatgcaggg gggaatctag tcttgaagta tccttattcc
3361 ttaaaaaaaa tattggacat cctcttgtgt tgttatccga tacaaccgga tatttttctt
3421 ttaatgagtc taaaccgtga tagcttcagg ttaatacaat caaaaaaagc tcaaatattc
3481 ttttaatgcc gcgttcacag attccaattg aatacaacta ggtagttcat tatatgaagc
3541 ctttgctact atttttcact atagtctgcc ttcaccttaa tgcagacatc cacatatttt
3601 aatcacttta aaataaaaag gaagatatat tagaagctat gatccaatct gtaagccaga
3661 ttaaaattca cgaactcttc tttcatttga attgaatgct ttgagttggg gtagattatc
3721 gcaaattact catcacattt attgactacg aacttgctga tgtccttttt ttatttatat
3781 ttttcttcag tgaagcgatt ttttttttac acagaccaag acggaaaaaa gtagctaagg
3841 aagaaaacaa aatcatgaaa aaaatgtgaa gtgatcatgc acatcgcatc aacttaaaca
3901 ttggcttaga gatatataga gttagagttt acggcaacct ttaagcacca ataccttttg
3961 gcatagtcta aagacctggt tcttaatttt aaacaaattt aactaaagat ttccctatca
4021 aagaagtaac gagttgacag attttctcaa aataaatcga tactgcattt ctaggcatat
4081 ccagcgagat ctatggatta caaggacaag gcattgaacg atttgagaaa cgtttatgca
4141 gacttcgact ctttaccatt agatttcaga caaatcttga ttaaagatag agcaactttg
4201 ttacaaaaag aagatgttga aaagaagatt ttagaaagac aggaagatgc taagaagtac
4261 gcagaatatt tgaaacaatc tgaaatccct gaaagaattt ctttaccaaa cattaaaaga
4321 cataagggtg tttcaatctc ttttgaagaa acttcagaag atatggtttt agaaccacgt
4381 ccttttattt ttgatggttt gaatatcaga tgttttagaa gggaaaccat cttctcattg
4441 aagaataaga tcctaaatat ggttaaggaa tcatcttctt tcaagaatgt ttctagacaa
4501 tcagtttctt tcatgtactt caagattttc aataaaggta aggttattgc ttcaactaaa
4561 tcagttaata tctacgaaga taaaattgat gaaagattag aagacttgtg taataatttt
4621 gatgatgttt tgaagaaaat tattgatgtt acttacggtt acgaatcttt atttgtttca
4681 gaaacatact cttatgttat tttctacgct aagtctatct atttcccaca accaagatgt
4741 gttaataatt ggggtaataa tattccaaac attttgactt tcgattcttt taaattgttt
4801 actgcaaata agaataatgt ttcatgtatt aaacaatgtt ctagattctt gtggcaaaag
4861 gattttaata cattggaaga aatgatcgaa tacaaaaacg gtaacatttg tattgttact
4921 ccacaattac atattaatga tgttagagac attaaatctt ttaatgatat cagattatac
4981 tctgaatccc caattaagac tttctctgtt attgacaaca caatcactta cttgttttat
5041 ttcaaagaac atttaggtgt tattttcaat attactaaat ctagacatga taggagagtt
5101 acaaagtttt ctccattgtc taaattttca gatgttaaaa atattacagt ttgttttgat
5161 attgaatcat atttcgatcc agaaaaagaa tcaaatcaag ttaatattcc ttttatttgt
5221 tgtgcttcta ttatctataa caaagttatc ggtaatattg ttgatttcga aggtagagat
5281 tgtgttgctc aaatgattga atatgttgtt gatatttgtg gtgaattaaa catttcatct
5341 gttgaattaa ttgctcataa tggtggtggt tacgatttcc attatatctt atcttctatg
5401 tataacccag cagctattaa aaatatctta atcagaaata attctttcat ttcatttaac
5461 tttgctcatg atggtgttaa attttctgtt aaagattcat actctttttt gttatgttca
5521 ttggctaatg catctaaagc ttttttgaat gaagaaacat ttaaaaaaac agattttcca
5581 catcacgatt tgaagactgc tgatgatttg tacaaggttt acaaagaatg gtcatctgtt
5641 aatactgaaa ttaatcatgt tgttgaaaaa gaaaaattgt taattacttc tgaacatatc
5701 gttaatttta ctaaaaatga taaatcaaaa acattaattg aatggtctaa agattattgt
5761 agaaatgatg ttttggtttt atcaaaagtt tggttagagt tcaagaacgc tgttgaagat
5821 attttcaatt gtgaattagt tgatcaaact atgacattgg caggtttgtc ttataaattg
5881 tttcaagcta atatgccatt tgatgttgaa ttaagacatc caaataaaga agattacttt
5941 aatatgagag aagctttaat cggtggtagg tgtatttcag ttaatggtat ttataaagat
6001 gttttgtgtt tagatgttaa atctttatac ccagcttcaa tggcattcta cgatcaacca
6061 tacggttcat ttaaaagagt ttctagcaga ccaaaggatg aattaggtat ctactatgtt
6121 agagttacac caaatagaaa taataagtca aatttctttc caatcagatc tcataacaaa
6181 atcacataca ataactttga agaatctact tacatcgctt ggtatactaa tgttgatatt
6241 gatatcggtt tgtctgaagg tcataacatt gaatatatcc cattcgattc ttacggtaat
6301 atcggttatt catggtctaa gaaaggtaag atcttcgaaa agtacatcaa agatgttttg
6361 tacaagttga agataaagta tgaaaaacaa aataataagg ttaaaagaaa tgttattaaa
6421 attattatga actctttgtg gggtaaattt gctcaaaaat gggttaactt cgaatatttt
6481 atcaaatctg aagatgatat cgattttgaa tctgaagaag cttacaagat ctgggatact
6541 gatttcatgt taattaagaa aattaaagaa tcaacatact catctaaacc aatccaaaat
6601 ggtgttttta cattatcatg ggctagatac cacatgaaat caatttggga tgctggtgca
6661 aaggaaggtg cagaatgtat atattctgat acagattcta tcttcgttca caaagaacat
6721 ttcaagaaaa atgctaaatt tatgttgaat ggtttgaagg ttccaattat tggttcagaa
6781 gttggtcaat tagaattgga atgtgaattt gataagttat tgtgtgcagg taagaaacaa
6841 tacatgggtt tctatacata ttttcaagat ggtaagccat gtattaaaga aaaaaagaga
6901 tttaagggta ttccatcaaa ttatatcatc ccagaattgt acgctcattt gttgtctggt
6961 gctgataaag aagcaaagat ccaattcttg aagtttagaa gggaatgggg ttctgttaaa
7021 ggttatattg aaaacaaaac tgttaaggca acataaattt ttcaaactgc aaattcaaga
7081 aaaagccacg cgtgtgcacc ttttttttcc ccttccagtg cattatgcaa tagacagcac
7141 gagtctttga aaaagtaact tataaaactg tatcaatttt taaacctaaa tagattcata
7201 aactattcgt taatataaag tgttctaaac tatgatgaaa aaataagcag aaaagactaa
7261 taattcttag ttaaaagcac tttatgctac aacattccaa aatttgtccc aaaaagtctt
7321 tggttcatga tcttcccata caattgcctc aatgtctctt ctatcggaat cgatgtcgac
7381 atctccaatc ttccaaataa atctgcatct gaatatgcat tgaaataaga tccaaacagc
7441 taagaacagg aaaatagaga tataggcggc agcaaagcta acaccattga attttggtgc
7501 aaaagccgtg aaaccttgaa taatgataat gatcgtcata aatgtggccg cataataagc
7561 caagccgggc attaatttag ctttaaatgg taactcgtca cgagagatgc cacggtattt
7621 caaagcttgc ataaatctga tgtgcgagat tgagataaat aaccatgcaa aaaagcctgc
7681 aacaccagtg atatttaata gccattcgaa aactttgtca ccaccagtag atgtctccat
7741 gtaagccaaa gcgccaaatg cagcagtaac gaaaactgca atgtatggaa caccaccttt
7801 ggtggtcctt gacaggaatt taggagccaa cttgttcttt gatagaccaa ataaaatacg
7861 ggaaccaacg taaatatttg aatttgcggc agaaataatg gttgttaaga taacagcgtt
7921 gaagatatgt ggcaaaacct ttgtaccaga gttctcaata gcaataataa agggagaagt
7981 agaaacgtag gaagtagatt gtgttagttt agggtcattg tatggaacta aaagtccaat
8041 gaataataga gagccaatgt agaaggttaa gatacggaaa acaacttttt tgatggctct
8101 tggaacggat tttctggggt ttgcagcttc accagcagtg ataccaacta gttcagtacc
8161 ttgaaatgtg aaggcagcgt taatcaaaga ggaaacccaa cctaagaacc tcccttcgtt
8221 tttatcctta gatattatac ctggacccca ggcacctggg tttctccaat aacggaatcc
8281 aactgggccg gtaaccccag caccacaaac catacaaaaa cagtatatta gaaacccgat
8341 aatggctaaa actttgatgg aagcgaccca gaactcaaat tcaccgtaat atttgacagg
8401 gaacaagttc attattgtga taattaccca aaaaatacta atccatgccg ccagtggaac
8461 tttgtacgtc caaaattgaa tgacttggcc aactacacta agttccaggg caaaagtgat
8521 tgcccaagaa aaccaataca tgtaaccatt ggccgcacca aatgctggag aaaggaatct
8581 ttgtgagaaa actgtgaaag aggatgtaac agggatgaat gtagccattt cacccaagga
8641 ctgcgtgaca gaatatgcca aagaacccat aaataaatat gatataagag cgcccactgg
8701 gccggcgttg gtcagaggtg tggataaacc aatgaaaaga cctgtaccaa tagtaccacc
8761 aagggcaatc ataccaatat gtctttgctt aagctctctc ttcacttcag cgttctgtac
8821 ttctccttca tcttcatcac ctatgccatc ctccatagag aacgtatcct cgccatttac
8881 tctcgtcggg aaagagcgca atggatacaa ttctttactt ttctcatctt tcaatggtat
8941 tgacccacgt ctgtggtgtg tttgtgaagc ttcaacgtcg tgaaagaggg ttgtgaccgg
9001 ctcattgtac atatgcttct cctctatgtc ggcgtcttct tttgaatttg tcatttcttc
9061 ccgttccagc actctatgtg attaagatcg agtgagcaaa cgtgtttttc taggatgatt
9121 tcttgaccaa ttctttgtgg agataagctt taaagtctga agaacgtatc tctgtagttc
9181 ttctgtctca tttaaaataa cccaaaattt ccgaaatttc agtgcagctc aaccctaaat
9241 tagacctaat tgaaatcagt agggttagct cgtatttgaa atattacaaa acaagtccta
9301 taaaactcta atcttaaccc ttatgttaga ttttcagcac gtgattttgc atctttttta
9361 gttttgctta aaaaattttg acctctacct tttctctctc tgtttaatca ggtaataaaa
9421 agttcagcca aaaagtgtgg ttgtgaaatt ttattctggc gatgcgcaat cggagtcttt
9481 tctatttctt gcaactcgca gaagaaaagg tattttacta cataaattgt atatattcct
9541 tctcaggatc agcaacataa gatattggct ttgatattat attgcatctt attgcattgt
9601 gtgttgtggt taagacaaat aaataaagga agtttcttct tttttttcct tccttttttt
9661 cctctctcag ataaaagttc aacagagcca tggtgtcgaa ggatcaaacg tcctttaata
9721 agaggtggac cttgggtcat ctgatgttgg gtcatgctat gccttttttt ttttttgttt
9781 ttacaggagt taagaagtct gaagaactct gaaataaact ttcgattgac gacagattaa
9841 aaactggatc caactataag tataatagta acttatatat ttctgttcca gatatatacg
9901 taaaaaaaag aaaaggataa ttgatgtttt tgcaaaaagg gattccctga ttcggtttac
9961 tctgttcgca accactcttc tttctgctaa tttgtgcttt atctcgtatc attctcttat
10021 ctacttatct taaccttatc gtcttcctcg aaattttcat tgatagagac aactgcaaaa
10081 aaagaaattc aaaaaagccg tatgactcac ccggaaacca cacagaaacc ctagatttct
10141 atagggcaaa tttcagggtt atactaaaag atatattatt gaccttggtt attcttcaag
10201 ttcgccctat tgaaaattcg gacactttag ggtttggaat ctgttaaaga tatagaattc
10261 actcaccaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta
10321 agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc
10381 gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg
10441 gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct cccgatccgt
10501 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
10561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
10621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
10681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
10741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
10801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
10861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
10921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
10981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
11041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
11101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
11161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
11221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
11281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
11341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt gagccctccc
11401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
11461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
11521 atatacttta gattgattta aaacttcatt tttaatttaa a
//
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