| 出品公司: | Clontech |
|---|---|
| 载体名称: | pQCXIX |
| 质粒类型: | 逆病毒载体；双顺反子载体；双表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6309 bp |
| 5' 测序引物及序列: | 5'd[ACGCCATCCACGCTGTTTTGACCT]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无标签 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α, HB101 |
| 宿主细胞（系）: | 常规细胞系 |
| 备注: | pQCXIX载体是双表达载体，含有IRES(内部核糖体结合位点）元件，两个MCS区，允许两个外源基因以双顺反子的形式表达。 |
| 产品目录号: | 631515 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pQCXIX 6291 bp DNA SYN
DEFINITION pQCXIX
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6291
/organism="pQCXIX"
/mol_type="other DNA"
promoter 2..507
/label="CMV_immearly_promoter"
misc_feature 6..689
/label="5_LTR"
misc_feature 9..297
/label="CAG_enhancer"
misc_feature 464..484
/label="CMV_fwd_primer"
misc_feature 759..1568
/label="psi_plus_pack"
misc_feature 1155..1562
/label="gag"
misc_feature 1498..1520
/label="pLXSN_5_primer"
misc_feature 1498..1520
/label="MSCV_primer"
misc_feature 1536..1552
/label="pBABE_5_primer"
misc_feature 1609..1896
/label="CAG_enhancer"
promoter 1614..2106
/label="CMV_immearly_promoter"
misc_feature 2063..2083
/label="CMV_fwd_primer"
promoter 2064..2133
/label="CMV_promoter"
promoter 2076..2195
/label="CMV2_promoter"
misc_feature 2107..2126
/label="pCEP_fwd_primer"
misc_feature 2109..2133
/label="LNCX_primer"
misc_feature 2290..2852
/label="IRES"
misc_feature complement(3525..3547)
/label="pGEX_3_primer"
misc_feature complement(3683..3703)
/label="pBABE_3_primer"
misc_feature complement(3689..3904)
/label="SV40_enhancer"
promoter 3701..3969
/label="SV40_promoter"
rep_origin 3868..3945
/label="SV40_origin"
misc_feature 3930..3949
/label="SV40pro_F_primer"
gene complement(5069..5929)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(5069..5929)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5971..5999)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 6221..507
/label="CMV_immearly_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6285..689
/label="5_LTR"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
1561 taggcgccgg aattgaagat ctgggggatc gatcctctag agtccgttac ataacttacg
1621 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
1681 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
1741 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
1801 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
1861 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
1921 tggcagtaca tcaatgggcg tgaatagcgg tttgactcac ggggatttcc aagtctccac
1981 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
2041 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
2101 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
2161 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
2221 acgctgcagg aattgatccg cggccgcacc ggtaggcctc gtacgcttaa ttaacggatc
2281 cggaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
2341 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
2401 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
2461 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
2521 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
2581 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
2641 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
2701 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
2761 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
2821 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgat cacgcgtctc
2881 gagatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
2941 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
3001 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
3061 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg
3121 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
3181 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
3241 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
3301 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
3361 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
3421 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
3481 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
3541 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
3601 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
3661 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
4021 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
4081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
4141 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4201 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4741 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
5401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
5701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6061 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
6121 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
6181 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
6241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//
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