pQCXIX

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pQCXIX
质粒类型: 逆病毒载体;双顺反子载体;双表达载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6309 bp
5' 测序引物及序列: 5'd[ACGCCATCCACGCTGTTTTGACCT]3'
3' 测序引物及序列: --
载体标签: 无标签
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系
备注: pQCXIX载体是双表达载体,含有IRES(内部核糖体结合位点)元件,两个MCS区,允许两个外源基因以双顺反子的形式表达。
产品目录号: 631515
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pQCXIX载体图谱



pQCXIX 多克隆位点

pQCXIX 载体特征

载体简介

载体序列

LOCUS       pQCXIX	6291 bp 	DNA	SYN
DEFINITION  pQCXIX
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="pQCXIX"
                     /mol_type="other DNA"
     promoter        2..507
                     /label="CMV_immearly_promoter"
     misc_feature    6..689
                     /label="5_LTR"
     misc_feature    9..297
                     /label="CAG_enhancer"
     misc_feature    464..484
                     /label="CMV_fwd_primer"
     misc_feature    759..1568
                     /label="psi_plus_pack"
     misc_feature    1155..1562
                     /label="gag"
     misc_feature    1498..1520
                     /label="pLXSN_5_primer"
     misc_feature    1498..1520
                     /label="MSCV_primer"
     misc_feature    1536..1552
                     /label="pBABE_5_primer"
     misc_feature    1609..1896
                     /label="CAG_enhancer"
     promoter        1614..2106
                     /label="CMV_immearly_promoter"
     misc_feature    2063..2083
                     /label="CMV_fwd_primer"
     promoter        2064..2133
                     /label="CMV_promoter"
     promoter        2076..2195
                     /label="CMV2_promoter"
     misc_feature    2107..2126
                     /label="pCEP_fwd_primer"
     misc_feature    2109..2133
                     /label="LNCX_primer"
     misc_feature    2290..2852
                     /label="IRES"
     misc_feature    complement(3525..3547)
                     /label="pGEX_3_primer"
     misc_feature    complement(3683..3703)
                     /label="pBABE_3_primer"
     misc_feature    complement(3689..3904)
                     /label="SV40_enhancer"
     promoter        3701..3969
                     /label="SV40_promoter"
     rep_origin      3868..3945
                     /label="SV40_origin"
     misc_feature    3930..3949
                     /label="SV40pro_F_primer"
     gene            complement(5069..5929)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(5069..5929)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5971..5999)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6221..507
                     /label="CMV_immearly_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6285..689
                     /label="5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
   61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
  121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
  181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
  241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
  301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
  361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
  421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
  481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
  541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
  601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
  661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
  721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
  781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
  841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
  901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
  961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
 1561 taggcgccgg aattgaagat ctgggggatc gatcctctag agtccgttac ataacttacg
 1621 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
 1681 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
 1741 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
 1801 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
 1861 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
 1921 tggcagtaca tcaatgggcg tgaatagcgg tttgactcac ggggatttcc aagtctccac
 1981 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
 2041 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
 2101 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
 2161 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
 2221 acgctgcagg aattgatccg cggccgcacc ggtaggcctc gtacgcttaa ttaacggatc
 2281 cggaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
 2341 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
 2401 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
 2461 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
 2521 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
 2581 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
 2641 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
 2701 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
 2761 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
 2821 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgat cacgcgtctc
 2881 gagatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
 2941 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
 3001 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
 3061 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg
 3121 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
 3181 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
 3241 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
 3301 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
 3361 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
 3421 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
 3481 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
 3541 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
 3601 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
 3661 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
 3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
 3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
 3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
 3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
 3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
 4021 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
 4081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
 4141 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
 4201 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
 4261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
 4321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
 4381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
 4441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
 4501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
 4561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
 4621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
 4681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
 4741 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
 4801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
 4861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
 4921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
 4981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
 5041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
 5101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
 5161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
 5221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
 5281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
 5341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
 5401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
 5461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
 5521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
 5581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
 5641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
 5701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
 5761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
 5821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
 5881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
 5941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
 6001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
 6061 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
 6121 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
 6181 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
 6241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t

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