| 质粒名称: | pXPR_011 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 59702 |
| 存储实验室: | John Doench, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8395 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFP sgRNA |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | hU6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | LKO.1 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXPR_011 was a gift from John Doench & David Root (Addgene plasmid # 59702 ; http://n2t.net/addgene:59702 ; RRID:Addgene_59702) |
| 参考文献部分: | Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt.3026 PubMed 25184501 |
买家导航
pXPR011载体基本信息
pXPR011载体图谱质粒图谱和多克隆位点信息
pXPR011载体简介
pXPR011载体序列
LOCUS pXPR_011 8395 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION This lentiviral vector can be used to assay Cas9 activity..
ACCESSION .
VERSION .
KEYWORDS pXPR_011
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8395)
AUTHORS Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I,
Sullender M, Ebert BL, Xavier RJ, Root DE
TITLE Rational design of highly active sgRNAs for CRISPR-Cas9-mediated
gene inactivation.
JOURNAL Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026.
PUBMED 25184501
REFERENCE 2 (bases 1 to 8395)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8395
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 7..507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 426..444
/label=hPGK-F
/note="Human PGK promoter, forward primer"
primer_bind complement(519..538)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1015..1035
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 1155..1220
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 1227..1949
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKS"
primer_bind complement(1272..1293)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1533..1552)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1880..1901
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1964..2552
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2017..2037)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2435..2446)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2624..2857
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2935..3056
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2972..2991)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3026..3045
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3096..3231
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3158..3177
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3251..3270)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3252..3270)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3280..3297)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3280..3296)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3289..3311)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3438..3893
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3525..3544)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3735..3756
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3919..4023
/gene="bla"
/label=AmpR promoter
CDS 4024..4884
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4242..4261)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5055..5643
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5544..5563
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5797..5814
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5931..5952
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5967..5997
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6005..6021
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6010..6032
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6029..6045
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6029..6045
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6064..6084
/label=T3
/note="T3 promoter, forward primer"
promoter 6066..6084
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6112..6338
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6339..6519
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6566..6691
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7184..7417
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7602..7646
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 7824..8064
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 7824..7844
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 7995..8014
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_feature 8226..8343
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
481 gaccgaatca ccgacctctc tccccagggg gatccaccat gaccgagtac aagcccaccg
541 tgcgcctcgc cacccgcgac gacgtgccca gggccgtccg caccctcgcc gccgctttcg
601 ccgactaccc cgccacccgc cacaccgtcg atcccgaccg ccacatcgag cgggtcaccg
661 agctgcaaga actcttcctc acccgcgtcg ggctcgacat cggcaaggtg tgggtcgctg
721 acgacggcgc cgcagtggct gtctggacca cacccgagag cgtcgaagct ggggcggtgt
781 tcgccgagat cggccctcgc atggccgagt tgagcggttc ccggctggcc gctcagcaac
841 agatggaagg cctcctggct ccacaccggc ccaaggagcc cgcatggttc ctggccaccg
901 tcggcgtctc tcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
961 tggaggctgc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgca ccccgcaacc
1021 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtggaggtg cccgaaggac
1081 cacgcacctg gtgcatgacc cgcaagcccg gtgccctgca gggatctggc gaagccagac
1141 acaaacagaa aattgtgaaa cagactttga attttgacct tctcaagttg gcgggagacg
1201 tggagtccaa cccagggccc gctagcatgg tgagcaaggg cgaggagctg ttcaccgggg
1261 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
1321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
1381 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
1441 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
1501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
1561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
1621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
1681 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
1741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
1801 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
1861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
1921 tcggcatgga cgagctgtac aagtcctaac gcgttaagtc gacaatcaac ctctggatta
1981 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
2041 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
2101 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
2161 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
2221 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
2281 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
2341 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
2401 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
2461 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
2521 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
2581 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
2641 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
2701 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
2761 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
2821 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
2881 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
2941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
3061 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
3241 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
3301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
3361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
3421 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
3481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
3541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
3601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
3661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
3721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
3781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
3841 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
3901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
3961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
4021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
4081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
4141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
4201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
4261 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
4321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
4381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
4441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
4501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
4561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
4621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
4681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
4741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
4801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
4861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
4921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
4981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
5041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
5101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
5161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
5221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
5281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
5341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
5401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
5461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
5521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
5581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
5641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
5701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
5761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
5821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
5881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
5941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
6001 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
6061 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
6121 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
6181 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
6241 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
6301 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
6361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
6421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
6481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
6541 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
6601 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
6661 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
6721 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
6781 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
6841 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
6901 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
6961 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
7021 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
7081 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
7141 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
7201 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
7261 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
7321 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
7381 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
7441 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
7501 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
7561 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
7621 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
7681 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
7741 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
7801 tcccaacccc gaggggaccc agagagggcc tatttcccat gattccttca tatttgcata
7861 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
7921 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
7981 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
8041 ctttatatat cttgtggaaa ggacgaaaca ccggtgaacc gcatcgagct gagtttaaga
8101 gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa
8161 gtggcaccga gtcggtgctt ttttgaattc tagatcttga gacaaatggc agtattcatc
8221 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
8281 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
8341 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga ggggg
//
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pXPR011载体质粒应用举例
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