| 质粒名称: | pAAVLINK-3'-EP300-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001688 |
| 产品用途: | 3' AAVLINK plasmid for EP300 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EP300 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-EP300-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-EP300-FLAG载体质粒基本信息
pAAVLINK-3'-EP300-FLAG质粒图谱载体图谱和pAAVLINK-3'-EP300-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-EP300-FLAG质粒载体简介
pAAVLINK-3'-EP300-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-EP300-FLAG 7183 bp DNA circular SYN 19-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7183)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7183
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3922
/codon_start=1
/label=EP300
/translation="VSFL*EVFQ*DPRGERFFGG*PFPASNYNK*RTIFQEKK*HTGS*
TVC*MYRVRKKDASDLCPSP*DHLACWIRL*WLFKEKCTN*ERK*VFC*KVAIYQTWHL
SRESCE*LSEATESP*VRRGHC*SSSCF*QNRGSKTRHESKVCGQWRDGRILSIPNQSP
LCL*RN*WC*PVLLWHACSRVWL*LPSTQPEESIHILPR*CSFLPS*MLEDCSLS*NPN
WIFRICQEIRLHNRAYLGMSTK*GR*LYLPLPSS*PEDTQAQATAGMVQKNA*QGCIRA
YCP*LQGYF*TSY*R*INKCKGIALFRG*FLAQCSGRKH*GTGTGGRREKTRGKHQQ*K
HRCDQGRQQKC*KEE**ENQQK*EQPE*GQQEETRDAQCI*RPLTETICHHGEA*RGLL
CDPPHCWPCCQLPASHC*S*SSHPLRSDGWSGCVSHAGKGQAPGVLFTPKSPVVHHVHA
GGAAHAEPGPLCLHLQ*MQAPCGDTLALYCL*GL*LVYHLL*H*KP*PQNGETRPWLR*
*EQQPAGCSHPEPRRFSPPEYPALHPVSGPCLPVSECQLLTAILPEDEAGCAAYQGLQT
ENQWRVPHLQAAHCPLLLPCQALPGEQMPGAVLPKHQAEAPAATAAAPTTAGPNASQED
GQHAADWCGWAATGPPFPHSCHSNDTNWPTANHPADAPAHFSASAYPSQ*HATLLAQDS
SCWPCVPG*GSRPGDPSNPSSDCSATPSRAPTCSSGNGNADSESSGDAAPDGPRANFSK
ANPTPDAPDDSHGPHGYEPTSHDQRSQWAFGARDGTDRDAATATLEPRRIASAPATTVW
DAKASHDVSGPAWSTFEHGSTTRIGPGRYQPTQTRHCVSTSLTKPFADSQVSQLSPAAA
TGA*YPSRQPPAVGCIHQAAGCQVCQL*STTHPWAAWHAPGAARATATYHARSAGGPLQ
SSHAEHESNAGGRSEGWPAPAATTAATPATHGRDEPPGSADEHEPQHHAFTIPRHLETT
ANDATAAATGSRARNRPWNGQP*PVPATPRSWLPTTAAAADAASHATDATRKYGTDRPA
SPGLGSRGRCQSTGLSAATPSATDGVPCSAQPHEPPAAYAPKSGPVPTPTRPADP*FSL
QSSALSPACPFSTATVPAPPLQSFPKDAASAFSTPRFPTDKFPTSWTGSCPGQPHGTRA
FCQPGPEFNAFSAC*QSRHGKPPWCKRHGPGTQHR*LRLEFKPLTEYTRHT"
misc_feature 1034..1039
/label=SDS
misc_feature 1245..1250
/label=SDS
regulatory 1436..1445
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 2168..2173
/label=SDS
misc_feature 2408..2413
/label=SDS
misc_feature 2671..2676
/label=SDS
CDS 3929..3994
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3998..4429
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4142..4147
/label=SDS
repeat_region 4446..4586
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4446..4575
/label=AAV2 ITR
rep_origin 4661..5116
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5398..5502
/gene="bla"
/label=AmpR promoter
CDS 5503..6363
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6534..7122
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtatcatttc tgtgagaagt gtttcaatga
301 gatccaaggg gagagcgttt ctttggggga tgacccttcc cagcctcaaa ctacaataaa
361 taaagaacaa ttttccaaga gaaaaaatga cacactggat cctgaactgt ttgttgaatg
421 tacagagtgc ggaagaaaga tgcatcagat ctgtgtcctt caccatgaga tcatctggcc
481 tgctggattc gtctgtgatg gctgtttaaa gaaaagtgca cgaactagga aagaaaataa
541 gttttctgct aaaaggttgc catctaccag acttggcacc tttctagaga atcgtgtgaa
601 tgactttctg aggcgacaga atcaccctga gtcaggagag gtcactgtta gagtagttca
661 tgcttctgac aaaaccgtgg aagtaaaacc aggcatgaaa gcaaggtttg tggacagtgg
721 agagatggca gaatcctttc cataccgaac caaagccctc tttgcctttg aagaaattga
781 tggtgttgac ctgtgcttct ttggcatgca tgttcaagag tatggctctg actgccctcc
841 acccaaccag aggagagtat acatatctta cctcgatagt gttcatttct tccgtcctaa
901 atgcttgagg actgcagtct atcatgaaat cctaattgga tatttagaat atgtcaagaa
961 attaggttac acaacagggc atatttgggc atgtccacca agtgagggag atgattatat
1021 cttccattgc catcctcctg accagaagat acccaagccc aagcgactgc aggaatggta
1081 caaaaaaatg cttgacaagg ctgtatcaga gcgtattgtc catgactaca aggatatttt
1141 taaacaagct actgaagata gattaacaag tgcaaaggaa ttgccttatt tcgagggtga
1201 tttctggccc aatgttctgg aagaaagcat taaggaactg gaacaggagg aagaagagag
1261 aaaacgagag gaaaacacca gcaatgaaag cacagatgtg accaagggag acagcaaaaa
1321 tgctaaaaag aagaataata agaaaaccag caaaaataag agcagcctga gtaggggcaa
1381 caagaagaaa cccgggatgc ccaatgtatc taacgacctc tcacagaaac tatatgccac
1441 catggagaag cataaagagg tcttctttgt gatccgcctc attgctggcc ctgctgccaa
1501 ctccctgcct cccattgttg atcctgatcc tctcatcccc tgcgatctga tggatggtcg
1561 ggatgcgttt ctcacgctgg caagggacaa gcacctggag ttctcttcac tccgaagagc
1621 ccagtggtcc accatgtgca tgctggtgga gctgcacacg cagagccagg accgctttgt
1681 ctacacctgc aatgaatgca agcaccatgt ggagacacgc tggcactgta ctgtctgtga
1741 ggattatgac ttgtgtatca cctgctataa cactaaaaac catgaccaca aaatggagaa
1801 actaggcctt ggcttagatg atgagagcaa caaccagcag gctgcagcca cccagagccc
1861 aggcgattct cgccgcctga gtatccagcg ctgcatccag tctctggtcc atgcttgcca
1921 gtgtcggaat gccaattgct cactgccatc ctgccagaag atgaagcggg ttgtgcagca
1981 taccaagggt tgcaaacgga aaaccaatgg cgggtgcccc atctgcaagc agctcattgc
2041 cctctgctgc taccatgcca agcactgcca ggagaacaaa tgcccggtgc cgttctgcct
2101 aaacatcaag cagaagctcc ggcagcaaca gctgcagcac cgactacagc aggcccaaat
2161 gcttcgcagg aggatggcca gcatgcagcg gactggtgtg gttgggcagc aacagggcct
2221 cccttccccc actcctgcca ctccaacgac accaactggc caacagccaa ccaccccgca
2281 gacgccccag cccacttctc agcctcagcc tacccctccc aatagcatgc caccctactt
2341 gcccaggact caagctgctg gccctgtgtc ccagggtaag gcagcaggcc aggtgacccc
2401 tccaacccct cctcagactg ctcagccacc ccttccaggg cccccacctg cagcagtgga
2461 aatggcaatg cagattcaga gagcagcgga gacgcagcgc cagatggccc acgtgcaaat
2521 ttttcaaagg ccaatccaac accagatgcc cccgatgact cccatggccc ccatgggtat
2581 gaacccacct cccatgacca gaggtcccag tgggcatttg gagccaggga tgggaccgac
2641 agggatgcag caacagccac cctggagcca aggaggattg cctcagcccc agcaactaca
2701 gtctgggatg ccaaggccag ccatgatgtc agtggcccag catggtcaac ctttgaacat
2761 ggctccacaa ccaggattgg gccaggtagg tatcagccca ctcaaaccag gcactgtgtc
2821 tcaacaagcc ttacaaaacc ttttgcggac tctcaggtct cccagctctc ccctgcagca
2881 gcaacaggtg cttagtatcc ttcacgccaa cccccagctg ttggctgcat tcatcaagca
2941 gcgggctgcc aagtatgcca actctaatcc acaacccatc cctgggcagc ctggcatgcc
3001 ccaggggcag ccagggctac agccacctac catgccaggt cagcaggggg tccactccaa
3061 tccagccatg cagaacatga atccaatgca ggcgggcgtt cagagggctg gcctgcccca
3121 gcagcaacca cagcagcaac tccagccacc catgggaggg atgagccccc aggctcagca
3181 gatgaacatg aaccacaaca ccatgccttc acaattccga gacatcttga gacgacagca
3241 aatgatgcaa cagcagcagc aacagggagc agggccagga ataggccctg gaatggccaa
3301 ccataaccag ttccagcaac cccaaggagt tggctaccca ccacagcagc agcagcggat
3361 gcagcatcac atgcaacaga tgcaacaagg aaatatggga cagataggcc agcttcccca
3421 ggccttggga gcagaggcag gtgccagtct acaggcctat cagcagcgac tccttcagca
3481 acagatgggg tcccctgttc agcccaaccc catgagcccc cagcagcata tgctcccaaa
3541 tcaggcccag tccccacacc tacaaggcca gcagatccct aattctctct ccaatcaagt
3601 gcgctctccc cagcctgtcc cttctccacg gccacagtcc cagccccccc actccagtcc
3661 ttccccaagg atgcagcctc agccttctcc acaccacgtt tccccacaga caagttcccc
3721 acatcctgga ctggtagctg cccaggccaa ccccatggaa caagggcatt ttgccagccc
3781 ggaccagaat tcaatgcttt ctcagcttgc tagcaatcca ggcatggcaa acctccatgg
3841 tgcaagcgcc acggacctgg gactcagcac cgataactca gacttgaatt caaacctctc
3901 acagagtaca ctagacatac actgtacaga ctacaaagac catgacggtg attataaaga
3961 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
4021 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
4081 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
4141 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
4201 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
4261 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
4321 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
4381 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaac ggaccgagcg
4441 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
4501 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
4561 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
4621 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
4681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
4741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4861 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4981 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
5041 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
5101 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
5161 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
5221 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
5281 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
5341 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
5401 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
5461 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
5521 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
5581 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
5641 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
5701 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5761 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5821 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5881 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5941 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
6001 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
6061 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
6121 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
6181 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
6241 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
6301 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
6361 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
6421 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
6481 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
6541 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
6601 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
6661 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
6721 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6781 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6841 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6901 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6961 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
7021 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
7081 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
7141 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINK-3'-EP300-FLAG质粒载体应用举例
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