pENTR3C

价格:800元

联系方式:I47-825O-882O

买家导航

pENTR 3C载体基本信息

出品公司: Invitrogen
载体名称: pENTR3C, pENTR 3C, pENTR-3C
质粒类型: Gateway载体;入门载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: Gateway
载体大小: 2723bp
5' 测序引物及序列: pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列: pENTR-R: ATGGCTCATAACACCCCTTG
载体标签: --
载体抗性: 卡那霉素
筛选标记: --
备注:
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须 使用DB3.1感受态细胞进行质粒扩增,用来构建Gateway系统入门载体。
 
产品目录号: 11817012
病毒/非病毒: 非病毒

pENTR 3C载体质粒图谱和多克隆位点信息

pENTR3C载体图谱



pENTR3C多克隆位点

pENTR3C载体特征

pENTR 3C载体简介

pENTR 3C载体序列

LOCUS       pENTR3C        2723 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     source          1..2723
                     /invitrogen="563"
                     /vntifkey="98"
     misc_recomb     358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(952..1051)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             618..923
                     /invitrogen="1060000"
                     /vntifkey="4"
                     /label=ccdB
     CDS             1174..1983
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      2047..2720
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
BASE COUNT      710 a       604 c       672 g       737 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatccg gtaccgaatt cgatcgctta ctaaaagcca gataacagta tgcgtatttg 
      541 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa 
      601 agaggtgtgc ttctagaatg cagtttaagg tttacaccta taaaagagag agccgttatc 
      661 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc 
      721 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc 
      781 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg 
      841 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta 
      901 acctgatgtt ctggggaata tagaattcgc ggccgcactc gagatatcta gacccagctt 
      961 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg 
     1021 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct 
     1081 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 
     1141 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg 
     1201 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 
     1261 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag 
     1321 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 
     1381 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 
     1441 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa 
     1501 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 
     1561 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 
     1621 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 
     1681 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 
     1741 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 
     1801 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 
     1861 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 
     1921 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 
     1981 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg 
     2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 
     2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     2701 tgctggcctt ttgctcacat gtt 
//

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