pYPQ202

LOCUS       pYPQ202               12807 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Multisite Gateway T-DNA entry plasmid (attR1   AtUBQ10 promoter and 
            Hygromiycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ202
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12807)
  AUTHORS   Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z,
            Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y
  TITLE     A CRISPR-Cpf1 system for efficient genome editing and 
            transcriptional repression in plants.
  JOURNAL   Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
  PUBMED    28211909
REFERENCE   2  (bases 1 to 12807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12807
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1125..1319
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1555..1577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1663..1803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1718..1737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1818..1835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1989..2577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2069..2088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2664..3458)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5082..5106
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5184..5358
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5399..6424)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6491..7167)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6534..6554)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7358..7379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7394..7424
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7432..7448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7437..7459
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7456..7472
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7456..7472
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      7489..7741
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7805..7929
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7970..8275)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8072..8091)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8595..9275)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     9190..9209
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9329..9359)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(9384..9508)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10191..10208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10191..10207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10200..10222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10410..10434
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11735..12364
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12793..1059
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
       61 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
      121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
      181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
      241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
      301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
      361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
      421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
      481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
      541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
      601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
      661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
      721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
      781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
      841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
      901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
      961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     1021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     1081 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
     1141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     1201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     1261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     1321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     1381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     1441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     1501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     1681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     1741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2641 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
     2701 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     2761 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     2821 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     2881 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     2941 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     3001 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     3061 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     3121 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     3181 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     3241 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     3301 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
     3361 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3421 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     3481 tacagtattt aaagataccc caagaagcta aggaaggtgc gaataagcgg ggaaattctt
     3541 ctcggctgac tcagtcattt catttcttca tgtttgagcc gattttttct cccgtaaatg
     3601 ccttgaatca gcctatttag accgtttctt cgccatttaa ggcgttatcc ccagttttta
     3661 gtgagatctc tcccactgac gtatcatttg gtccgcccga aacaggttgg ccagcgtgaa
     3721 taacatcgcc agttggttat cgtttttcag caaccccttg tatctggctt tcacgaagcc
     3781 gaactgtcgc ttgatgatgc gaaatgggtg ctccaccctg gcccggatgc tggctttcat
     3841 gtattcgatg ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt
     3901 gccggggcgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
     3961 cgccccttgg tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc
     4021 cagctgattg aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact
     4081 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
     4141 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat
     4201 gatggtggca tcgaccaagg tgccttgagt catcatgacg cctgcttcgg ccagccagcg
     4261 attgatggtc ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt
     4321 catgatggtg gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat
     4381 ggaggcgatt tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg
     4441 catgcagtga atgcgtagca tggtttccag cggataaggt cgccggccat taccagcctt
     4501 ggggtaaaac ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg
     4561 ggacaagaaa atctcttttc tggtctgacg gcgcttactg ctgaattcac tgtcggcgaa
     4621 ggtaagttga tgactcatga tgaaccctgt tctatggctc cagatgacaa acatgatctc
     4681 atatcaggga cttgttcgca ccttccctaa ttataacaag acgaactcca attcactgtt
     4741 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     4801 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     4861 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     4921 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     4981 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     5041 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     5101 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     5161 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     5221 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     5281 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     5341 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     5401 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     5461 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     5521 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     5581 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     5641 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     5701 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     5761 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     5821 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     5881 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     5941 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     6001 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     6061 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
     6121 cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact
     6181 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     6241 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     6301 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     6361 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     6421 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
     6481 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     6541 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     6601 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6661 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6721 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6781 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6841 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6901 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     6961 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     7021 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     7081 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     7141 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     7201 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     7261 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7321 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7381 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7441 gataacaatt tcacacagga aacagctatg acatgattac gaattcccga tctagtaaca
     7501 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     7561 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     7621 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     7681 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     7741 cggggaaatt cgagctccac cgcggtggcg gccgctctag aactagttaa ttaagaatta
     7801 tcgaaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
     7861 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     7921 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     7981 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     8041 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     8101 catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg
     8161 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     8221 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     8281 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     8341 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     8401 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     8461 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     8521 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     8581 ctgttatctg gcttttagta agccggatcc tctagattac gccccgcctg ccactcatcg
     8641 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     8701 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     8761 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     8821 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     8881 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     8941 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     9001 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat
     9061 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     9121 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     9181 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     9241 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     9301 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
     9361 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
     9421 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     9481 ctcgttcagc ttttttgtac aaacttgttt gatagcttgg cgcgcctcga gggggggccc
     9541 ggtaccctgt taatcagaaa aactcagatt aatctacaaa ttcgatcgca caaactagaa
     9601 actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa
     9661 attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct
     9721 aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac
     9781 atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac
     9841 agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa
     9901 ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga
     9961 agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa
    10021 taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt
    10081 atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc
    10141 ggctgcaacc actttgacgc cgtttattac tgactcgtcg aaagcttggc actggccgtc
    10201 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10261 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10321 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
    10381 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
    10441 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
    10501 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
    10561 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
    10621 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
    10681 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
    10741 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
    10801 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
    10861 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
    10921 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
    10981 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
    11041 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
    11101 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
    11161 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
    11221 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
    11281 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
    11341 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
    11401 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
    11461 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
    11521 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
    11581 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
    11641 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
    11701 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
    11761 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
    11821 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
    11881 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
    11941 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
    12001 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
    12061 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
    12121 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
    12181 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
    12241 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
    12301 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
    12361 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
    12421 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
    12481 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    12541 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    12601 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
    12661 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
    12721 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
    12781 cgaggaatcg gcgtgagcgg tcgcaaa
//