pAdTrack

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pAdTrack
质粒类型: 哺乳动物细胞腺病毒表达载体
高拷贝/低拷贝: 低拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8175 bp
5' 测序引物及序列: /
3' 测序引物及序列: /
载体标签: EGFP
载体抗性: 卡那
备注: pAdTrack载体用来转基因腺病毒细胞表达,载体拥有一个EGFP绿色荧光蛋白跟踪基因表达结果,和pAdeasy1载体共同使用时最大能够插入5.9kb,pAdeasy2载体共同使用时能够最大插入8.6kb片段。具体使用方法可以参考pAdeasy载体系统。
产品目录号: 240005
稳定性: /
组成型: --
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAdTrack载体图谱


载体简介

载体序列

LOCUS       pAdTrack	8175 bp 	DNA     circular	SYN
DEFINITION  pAdTrack
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="pAdTrack"
                     /mol_type="other DNA"
     misc_feature    197..345
                     /label="Encap"
     misc_feature    complement(374..393)
                     /label="EBV_rev_primer"
     CDS             575..1354
                     /label="ORF frame 2"
     CDS             complement(593..1339)
                     /label="ORF frame 3"
     gene            complement(623..1336)
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(665..686)
                     /label="EGFP_C_primer"
     misc_feature    complement(1127..1156)
                     /label="Y66 (EGFP)"
     misc_feature    1273..1294
                     /label="EGFP_N_primer"
     misc_feature    complement(1413..1433)
                     /label="CMV_fwd_primer"
     CDS             complement(2563..3921)
                     /label="ORF frame 1"
     CDS             complement(3669..4190)
                     /label="ORF frame 2"
     misc_feature    complement(5147..5242)
                     /label="LITR"
     misc_feature    5147..5242
                     /label="RITR"
     rep_origin      complement(5487..6106)
                     /label="pBR322_origin"
     gene            complement(6261..6560)
                     /label="Ampicillin (299 - 0)"
                     /gene="Ampicillin (299 - 0)"
     promoter        6765..6814
                     /label="NEOKAN_promoter"
     CDS             6903..7697
                     /label="ORF frame 3"
     gene            6906..7694
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
ORIGIN
    1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA
   61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GGCGTGGGAA CGGGGCGGGT GACGTAGTAG
  121 TGTGGCGGAA GTGTGATGTT GCAAGTGTGG CGGAACACAT GTAAGCGACG GATGTGGCAA
  181 AAGTGACGTT TTTGGTGTGC GCCGGTGTAC ACAGGAAGTG ACAATTTTCG CGCGGTTTTA
  241 GGCGGATGTT GTAGTAAATT TGGGCGTAAC CGAGTAAGAT TTGGCCATTT TCGCGGGAAA
  301 ACTGAATAAG AGGAAGTGAA ATCTGAATAA TTTTGTGTTA CTCATAGCGC GTAANNNCGC
  361 GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT
  421 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA
  481 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT
  541 TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG
  601 ATCCGGTGGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
  661 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
  721 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
  781 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
  841 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
  901 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
  961 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
 1021 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
 1081 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT
 1141 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA
 1201 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
 1261 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
 1321 CCTCGCCCTT GCTCACCATG GTGGCGACCG GTAGCGCTAG CGGATCTGAC GGTTCACTAA
 1381 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
 1441 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
 1501 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
 1561 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
 1621 CTGCCAAGTA GGAAAGTCCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
 1681 ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA
 1741 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT
 1801 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
 1861 AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA
 1921 TGACCCCGTA ATTGATTACT ATTANNNCTA GCAGATCTGG TACCGTCGAC GCGGCCGCGA
 1981 TATCCTCGAG AAGCTTTCTA GAGNNNTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT
 2041 TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA
 2101 TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA
 2161 GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT
 2221 GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC
 2281 CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG
 2341 CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT
 2401 GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA
 2461 GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC
 2521 AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG
 2581 CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG
 2641 GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG
 2701 GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA
 2761 GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG
 2821 CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA
 2881 TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG
 2941 GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC
 3001 ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT
 3061 TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT
 3121 ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT
 3181 TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC
 3241 GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC
 3301 TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG
 3361 CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC
 3421 CGGGTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC
 3481 TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC
 3541 GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC
 3601 CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT
 3661 CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT
 3721 TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG
 3781 CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG
 3841 CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC
 3901 TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG
 3961 CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG
 4021 AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG
 4081 GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA
 4141 ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT
 4201 CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTNNNG TTTAAACGAA
 4261 TTCNNNTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA
 4321 AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA
 4381 AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA
 4441 TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA
 4501 AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA
 4561 AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC
 4621 ACATCAGGTT GATTCATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA
 4681 TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG
 4741 AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG
 4801 CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA
 4861 AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA GCTCAATCAG TCACAGTGTA
 4921 AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA AAATGACGTA ACGGTTAAAG
 4981 TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA
 5041 ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTAACT TCCCATTTTA
 5101 AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC GTCACCCGCC
 5161 CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC TCATTATCAT ATTGGCTTCA
 5221 ATCCAAAATA AGGTATATTA TTGATGATNN NTTAATTAAG GATCCNNNCG GTGTGAAATA
 5281 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT
 5341 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
 5401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
 5461 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
 5521 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
 5581 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
 5641 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
 5701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
 5761 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
 5821 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
 5881 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
 5941 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
 6001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
 6061 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
 6121 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
 6181 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
 6241 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
 6301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
 6361 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
 6421 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
 6481 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
 6541 CCAGTTAATA GTTTGCGCAA CGTTGTTGNN NNNNAAAAAG GATCTTCACC TAGATCCTTT
 6601 TCACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG
 6661 CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA
 6721 CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG
 6781 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTCGCCGC
 6841 CAAGGATCTG ATGGCGCAGG GGATCAAGCT CTGATCAAGA GACAGGATGA GGATCGTTTC
 6901 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
 6961 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
 7021 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
 7081 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
 7141 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
 7201 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
 7261 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
 7321 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
 7381 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
 7441 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
 7501 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
 7561 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
 7621 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
 7681 TTGACGAGTT CTTCTGAATT TTGTTAAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
 7741 TAGGCCGAAA TCGGCAACAT CCCTTATAAA TCAAAAGAAT AGACCGCGAT AGGGTTGAGT
 7801 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
 7861 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT
 7921 TTGCGGTCGA GGTGCCGTAA AGCTCTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
 7981 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
 8041 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCGCGC
 8101 TTAATGCGCC GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 8161 NNNNNNNNNN NNNNN
//

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载体应用举例

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