pSTAT3-TA-Luc

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

载体名称: pSTAT3-TA-Luc
质粒类型: 哺乳动物细胞载体;信号通路载体
复制子:
pUC ori,SV40 ori
克隆方法: --
启动子: SV40
终止子: SV40 poly (A) signal
载体大小: 5672dp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
培养条件: 37°C ,LB培养基
克隆菌株: DH5α 等
宿主细胞(系): 哺乳动物细胞
备注: --
产品目录号: --
稳定性: --
诱导方式: 无诱导,瞬时表达
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pSTAT3-TA-luc载体图谱
pSTAT3-TA-luc载体图谱1

载体简介

PSTAT3-TA-luc可以通过传统的细胞转染方法转染到细胞中。碧云田萤火虫萤火虫荧光素酶报告基因检测试剂盒(RG005/RG006)或双荧光素酶报告基因检测试剂盒(RG027/RG028)。 STAT3试剂可活化,可作为阳性对照进行pSTAT3 TA-luc报告基因检测。

载体序列

LOCUS       pSTAT3-TA-luc                5672 bp ds-DNA     circular SYN 04-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        109..140
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             173..1825
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1869..1990
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2184..2541
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2392..2527
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2572..3366
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3391..3439
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3766..4354)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4554..5414)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5519..5567
                     /note="synthetic polyadenylation signal"
     misc_feature    5581..5672
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt
       61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat
      121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
      181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
      241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
      301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
      361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
      421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
      481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
      541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
      601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
      661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
      721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
      781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
      841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
      901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
      961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
//

      

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