| 载体名称: | pSTAT3-TA-Luc |
|---|---|
| 质粒类型: | 哺乳动物细胞载体；信号通路载体 |
| 复制子: |
pUC ori，SV40 ori
|
| 克隆方法: | -- |
| 启动子: | SV40 |
| 终止子: | SV40 poly (A) signal |
| 载体大小: | 5672 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | EBV-R: GTGGTTTGTCCAAACTCATC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 培养条件: | 37°C ,LB培养基 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 诱导方式: | 无诱导，瞬时表达 |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
PSTAT3-TA-luc可以通过传统的细胞转染方法转染到细胞中。碧云田萤火虫萤火虫荧光素酶报告基因检测试剂盒（RG005/RG006）或双荧光素酶报告基因检测试剂盒（RG027/RG028）。 STAT3试剂可活化，可作为阳性对照进行pSTAT3 TA-luc报告基因检测。
载体序列
LOCUS pStat3-luc 5672 bp ds-DNA circular SYN 22-DEC-2021
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5672)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, Dec 22, 2021 from SnapGene Viewer 5.1.4.1 to
Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|969580800|
COMMENT VNTDBDATE|969580800|
COMMENT VNTNAME|pStat3-luc|
FEATURES Location/Qualifiers
promoter 109..140
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 167..176
/regulatory_class="other"
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 173..1825
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
polyA_signal 1869..1990
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2184..2541
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2392..2527
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2572..3366
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
polyA_signal 3391..3439
/label=poly(A) signal
/note="synthetic polyadenylation signal"
rep_origin complement(3766..4354)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4554..5414)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
polyA_signal 5519..5567
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 5581..5672
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt
61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat
121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
1441 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa
1501 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa
1561 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc
1621 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc
1681 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc
1741 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa
1801 gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc
1861 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
1921 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
1981 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
2041 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt
2101 tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa
2161 agaggaactt ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
2221 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2341 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2461 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
2521 ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgatcgaa
2581 caagacggcc tccatgctgg cagtcccgca gcttgggtcg aacgcttgtt cgggtacgac
2641 tgggcccagc agaccatcgg atgtagcgat gcggccgtgt tccgtctaag cgctcaaggc
2701 cggcccgtgc tgttcgtgaa gaccgacctg agcggcgccc tgaacgagct tcaagacgag
2761 gctgcccgcc tgagctggct ggccaccacc ggcgtaccct gcgccgctgt gttggatgtt
2821 gtgaccgaag ccggccggga ctggctgctg ctgggcgagg tccctggcca ggatctgctg
2881 agcagccacc ttgcccccgc tgagaaggtt tctatcatgg ccgatgcaat gcggcgcctg
2941 cacaccctgg accccgctac ctgccccttc gaccaccagg ctaagcatcg gatcgagcgt
3001 gctcggaccc gcatggaggc cggcctggtg gaccaggacg acctggacga ggagcatcag
3061 ggcctggccc ccgctgaact gttcgcccga ctgaaagccc gcatgccgga cggtgaggac
3121 ctggttgtca cacacggaga tgcctgcctc cctaacatca tggtcgagaa tggccgcttc
3181 tccggcttca tcgactgcgg tcgcctagga gttgccgacc gctaccagga catcgccctg
3241 gccacccgcg acatcgctga ggagcttggc ggcgagtggg ccgaccgctt cttagtcttg
3301 tacggcatcg cagctcccga cagccagcgc atcgccttct accgcttgct cgacgagttc
3361 ttttaatgat ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
3421 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
3481 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
3541 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
3601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
3721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
3781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
4021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
4321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
4381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
4441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
4501 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
4561 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
4621 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
4681 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
4741 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
4801 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
4861 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
4921 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
4981 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
5041 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
5101 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
5161 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
5221 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
5281 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
5341 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
5401 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
5461 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
5521 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
5581 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
5641 cagtgcaagt gcaggtgcca gaacatttct ct
//
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