pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BLM-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BLM-FLAG               6413 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3126
                     /codon_start=1
                     /label=BLM
                     /translation="G*FTASPGENVTPWLTRYREMGSLLLLTMLASVILPEMKCSRSGL
                     IRMAVRLSVLQLHLEWGLTNRTCDL*FMHLSLNLWRVTTKNLAELEEMGKYLTACFSIP
                     IMM*PD*KDL**WKKMETIIQEKLTSIICIAWYITVKI*RNAGEYSFWPTLVKMDLILI
                     FVRNTQMFLVIIAVKQRIIKQEM*LTM*KVL*DLFKNIVHHKE*EI*NM*VLLEDLL*I
                     CWSTFSWGVRVQKSSQVYLEKDLLIHDTMPKDFLKS*YLTRFWMKTYISMPMTRRSLM*
                     CSEIKPKLY*MAI*R*TLWKQKIPAV*KNKKR**QKCLRGKRWLKNVLENLQKSANLWG
                     KFLVSITSIFLIPSLSRSLQNLYLLILRFCFKLMVLLKTNWKNMVRK*FQYYRNTLNGH
                     RQLKTVPQG*ACPAAEAPEEVPLRSSTRKYPYLPTTLQVKPEMKGRGKRCQPPKGLRGE
                     KLLPVVPRQRGGLPHVERYLPKRNPPASLDPVQPHILLKRHQEPIANWGLWLHRSL*ID
                     RFLSLHMHS"
     CDS             3133..3198
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3202..3633
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3346..3351
                     /label=SDS
     repeat_region   3676..3816
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3676..3805
                     /label=AAV2 ITR
     rep_origin      3891..4346
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4628..4732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4733..5593
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5764..6352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggat aatttactgc
     1561 ctctccaggc gagaatgtga caccatggct gacacgttac agagagatgg gctcgctgct
     1621 cttgcttacc atgctggcct cagtgattct gccagagatg aagtgcagca gaagtggatt
     1681 aatcaggatg gctgtcaggt tatctgtgct acaattgcat ttggaatggg gattgacaaa
     1741 ccggacgtgc gatttgtgat tcatgcatct ctccctaaat ctgtggaggg ttactaccaa
     1801 gaatctggca gagctggaag agatggggaa atatctcact gcctgctttt ctatacctat
     1861 catgatgtga ccagactgaa aagacttata atgatggaaa aagatggaaa ccatcataca
     1921 agagaaactc acttcaataa tttgtatagc atggtacatt actgtgaaaa tataacggaa
     1981 tgcaggagaa tacagctttt ggcctacttt ggtgaaaatg gatttaatcc tgatttttgt
     2041 aagaaacacc cagatgtttc ttgtgataat tgctgtaaaa caaaggatta taaaacaaga
     2101 gatgtgactg acgatgtgaa aagtattgta agatttgttc aagaacatag ttcatcacaa
     2161 ggaatgagaa atataaaaca tgtaggtcct tctggaagat ttactatgaa tatgctggtc
     2221 gacattttct tggggagtaa gagtgcaaaa atccagtcag gtatatttgg aaaaggatct
     2281 gcttattcac gacacaatgc cgaaagactt tttaaaaagc tgatacttga caagattttg
     2341 gatgaagact tatatatcaa tgccaatgac caggcgatcg cttatgtgat gctcggaaat
     2401 aaagcccaaa ctgtactaaa tggcaattta aaggtagact ttatggaaac agaaaattcc
     2461 agcagtgtga aaaaacaaaa agcgttagta gcaaaagtgt ctcagaggga agagatggtt
     2521 aaaaaatgtc ttggagaact tacagaagtc tgcaaatctc tggggaaagt ttttggtgtc
     2581 cattacttca atatttttaa taccgtcact ctcaagaagc ttgcagaatc tttatcttct
     2641 gatcctgagg ttttgcttca aattgatggt gttactgaag acaaactgga aaaatatggt
     2701 gcggaagtga tttcagtatt acagaaatac tctgaatgga catcgccagc tgaagacagt
     2761 tccccaggga taagcctgtc cagcagcaga ggccccggaa gaagtgccgc tgaggagctc
     2821 gacgaggaaa tacccgtatc ttcccactac tttgcaagta aaaccagaaa tgaaaggaag
     2881 aggaaaaaga tgccagcctc ccaaaggtct aagaggagaa aaactgcttc cagtggttcc
     2941 aaggcaaagg gggggtctgc cacatgtaga aagatatctt ccaaaacgaa atcctccagc
     3001 atcattggat ccagttcagc ctcacatact tctcaagcga catcaggagc caatagcaaa
     3061 ttggggatta tggctccacc gaagcctata aatagaccgt ttcttaagcc ttcatatgca
     3121 ttctcatgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
     3181 aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa
     3241 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3301 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3361 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3421 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
     3481 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
     3541 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3601 caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc
     3661 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3721 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3781 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3841 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3961 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4021 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4081 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4141 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4201 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4261 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4321 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4381 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4441 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4501 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4561 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5881 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//