| 质粒名称: | pHR_PGK_antiCD19_synNotch_TetRVP64 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 71926 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR PGK |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | antiCD19_synNotch_TetRVP64 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_PGK_antiCD19_synNotch_TetRVP64 was a gift from Wendell Lim (Addgene plasmid # 79126 ; http://n2t.net/addgene:79126 ; RRID:Addgene_79126) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_PGK_antiCD19_synNotch_TetRVP64 11529 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral vector for constitutive expression of the anti-CD19 scFv
synNotch TetRVP64 Receptor (PGK promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_PGK_antiCD19_synNotch_TetRVP64
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11529)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 11529)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11529
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 61..294
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(319..337)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(320..337)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(627..646)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 980..1002
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1040..1058)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1126..1230
/gene="bla"
/label=AmpR promoter
CDS 1231..2091
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1449..1468)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2262..2850
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2751..2770
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3004..3021
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3091..3111)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3096..3425
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3276..3411
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3338..3357
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4559..4624
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4754..4774
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5199..5333
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5236..5255)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5290..5309
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5502..6135
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6182..6307
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6799..7032
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 7544..7661
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7721..8220
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7756..7779)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 8153..8172
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 8328..8357
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 10062..10082
/gene="mutant version of attB"
/label=attB5
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 10083..10829
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=tTA-Advanced
/note="In the Tet-Off(R) system, tTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 10939..11527
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(10992..11012)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(11410..11421)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
7681 gcagagatcc agtttggatc gataagcttg atatcgaatt gggtagggga ggcgcttttc
7741 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
7801 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
7861 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
7921 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
7981 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
8041 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
8101 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
8161 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
8221 aattctcacg cgtcaagtgg agcaaggcag gtggacagtg gatcatggcg ctccctgtca
8281 ccgcactgct tcttccgctg gcactgctgc tgcacgctgc acggcctgag caaaaactta
8341 tctctgaaga ggacctcgat atacagatga cgcagacaac gtcaagtctt tccgccagct
8401 tgggagaccg agtgactata tcttgtagag caagccagga tatttctaag tatcttaact
8461 ggtaccaaca aaagcccgat ggaacggtta agctgcttat ataccatacc agtagactcc
8521 actccggcgt accatcacgg ttttctggca gtggctccgg gaccgactat tctttgacga
8581 tctctaatct cgaacaagag gatattgcaa catacttttg tcagcaaggc aataccttgc
8641 catatacgtt tgggggcggg acaaaacttg agataaccgg cggcggtggt tcaggcggtg
8701 gcggttccgg tggtggggga tcagaggtta agcttcagga atccggacca ggtttggttg
8761 cccccagcca atctctcagc gttacatgca cggtttcagg cgtcagtctc cccgattacg
8821 gtgtaagttg gattcggcaa cctccgcgaa agggtctgga atggctgggg gttatttggg
8881 ggagtgagac aacttattac aactctgcac ttaagagtcg gcttaccatc atcaaggata
8941 attcaaaatc acaagtattc ctgaagatga actcattgca aacagatgat acagctatat
9001 actattgtgc caagcattac tattatggtg gttcttatgc aatggattac tgggggcaag
9061 gcacgtcagt gacagtgagt tcaatcctgg actacagctt cacaggtggc gctgggcgcg
9121 acattccccc accgcagatt gaggaggcct gtgagctgcc tgagtgccag gtggatgcag
9181 gcaataaggt ctgcaacctg cagtgtaata atcacgcatg tggctgggat ggtggcgact
9241 gctccctcaa cttcaatgac ccctggaaga actgcacgca gtctctacag tgctggaagt
9301 attttagcga cggccactgt gacagccagt gcaactcggc cggctgcctc tttgatggct
9361 tcgactgcca gctcaccgag ggacagtgca accccctgta tgaccagtac tgcaaggacc
9421 acttcagtga tggccactgc gaccagggct gtaacagtgc cgaatgtgag tgggatggcc
9481 tagactgtgc tgagcatgta cccgagcggc tggcagccgg caccctggtg ctggtggtgc
9541 tgcttccacc cgaccagcta cggaacaact ccttccactt tctgcgggag ctcagccacg
9601 tgctgcacac caacgtggtc ttcaagcgtg atgcgcaagg ccagcagatg atcttcccgt
9661 actatggcca cgaggaagag ctgcgcaagc acccaatcaa gcgctctaca gtgggttggg
9721 ccacctcttc actgcttcct ggtaccagtg gtgggcgcca gcgcagggag ctggacccca
9781 tggacatccg tggctccatt gtctacctgg agatcgacaa ccggcaatgt gtgcagtcat
9841 cctcgcagtg cttccagagt gccaccgatg tggctgcctt cctaggtgct cttgcgtcac
9901 ttggcagcct caatattcct tacaagattg aggccgtgaa gagtgagccg gtggagcctc
9961 cgctgccctc gcagctgcac ctcatgtacg tggcagcggc cgccttcgtg ctcctgttct
10021 ttgtgggctg tggggtgctg ctgtcccgca agcgccggcg gcaactttgt atacaaaagt
10081 tgatgtctag actggacaag agcaaagtca taaactctgc tctggaatta ctcaatgaag
10141 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
10201 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctggca atcgagatgc
10261 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
10321 ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
10381 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
10441 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
10501 ttacactggg ctgcgtattg gaggatcagg agcatcaagt agcaaaagag gaaagagaga
10561 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccatc
10621 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
10681 agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc
10741 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
10801 attttgacct tgacatgctc cccgggtaag atccttgact tgcggccgca actcccacct
10861 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt
10921 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
10981 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
11041 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
11101 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
11161 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
11221 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
11281 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
11341 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
11401 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
11461 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
11521 tccccgcat
//
