| 质粒名称: | pST1374-NLS-flag-linker-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 44758 |
| 存储实验室: | Xingxu Huang |
|
载体骨架基本信息 |
|
| 载体名称: | pST1374 (Addgene #13426) |
| 载体出品公司: | pST1374-based custom vector |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5052 bp |
| 完整质粒大小: | 9318 bp |
| 载体类型: | 哺乳细胞表达，CRISPR |
| 筛选标记: | 稻瘟 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Human codon optimized Cas9 |
| 插入大小: | 4266 bp |
| 基因突变: | codon-optimized |
| 启动子 | CMV |
| 标签/融合蛋白: |
Flag (N terminal on insert)
NLS (N terminal on insert)
32 amino acid linker (N terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI(未破坏) |
| 3' 克隆位点: | ApaI(未破坏) |
| 5' 测序引物 | CMV-fwd |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pST1374-NLS-flag-linker-Cas9 was a gift from Xingxu Huang (爱迪基因 plasmid # 44758) |
| 参考文献部分: | Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X. Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46. 10.1038/cr.2013.46 PubMed 23545779 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pST1374-NLS-flag-linker-Cas9 9317 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pST1374-NLS-flag-linker-Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9317)
AUTHORS Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X
TITLE Generation of gene-modified mice via Cas9/RNA-mediated gene
targeting.
JOURNAL Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46.
PUBMED 23545779
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pST1374-NLS-flag-linker-Cas9|
FEATURES Location/Qualifiers
source 1..9317
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 270..391
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(281..300)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 335..354
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(549..947)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
promoter complement(966..1013)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(1061..1390)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1075..1210
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1129..1148)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 1375..1395
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin complement(1404..1832)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1514..1535)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1726..1745
/label=F1ori-R
/note="F1 origin, reverse primer"
polyA_signal 1878..2102
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 2091..2108
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(2131..2148)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(2158..2199)
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS complement(2212..6315)
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(6418..6441)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS complement(6451..6471)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(6499..6518)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6500..6518)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(6563..6766)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(6592..6612)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 6767..7146
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 7318..7337
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 7412..7516
/gene="bla"
/label=AmpR promoter
CDS 7517..8377
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7735..7754)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8548..9136
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9037..9056
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9290..9307
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
541 cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat cccaactgcc
601 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
661 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
721 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
781 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
841 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
901 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc
961 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
1021 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
1081 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
1141 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
1201 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
1261 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
1321 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
1381 actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt
1441 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
1501 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
1561 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
1621 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
1681 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
1741 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
1801 cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt
1861 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
1921 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
1981 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
2041 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
2101 agtcgaggct gatcagcggg tttaaactca atggtgatgg tgatgatgac cggtacgcgt
2161 agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaagggcc cctagtcgcc
2221 gccgagctga gacaggtcga tgcgagtctc gtacagtccg gtaattgact ggtgtatcag
2281 ggtggcatcg agaacttctt tggtagaggt gtaccgcttc ctgtcaatag ttgtatcgaa
2341 atacttgaag gcagcaggag cgcccagatt agtcagagta aagaggtgga taatattctc
2401 tgcttgttcg cgaattggct tgtccctgtg cttattatat gcgctcagca ctttatcgag
2461 gtttgcatcg gccagaataa cccgcttgct gaactcgcta atctgttcaa tgatttcgtc
2521 caggtagtgt ttatgttgct caacaaagag ttgcttctgc tcattgtctt cagggctacc
2581 tttgagtttc tcgtagtggg aggccagata caggaagttc acgtatttgg agggcagagc
2641 cagctcgttt cctttctgca gctctccggc ggaggccagc atccgcttcc taccattctc
2701 cagctcaaag agagagtact tgggcagttt gatgatgaga tctttcttca cttctttata
2761 gcccttagct tccaggaaat cgattggatt cttctcgaag ctggatctct ccataatagt
2821 aattccgagc agctccttaa cagacttgag tttcttggac ttgcctttct ccacttttgc
2881 cacgaccaga acggaataag ccactgtagg ggaatcgaaa ccgccatact tctttgggtc
2941 ccaatctttc ttcctggcga tcagcttgtc agagttccgc tttggcagga tgctctcctt
3001 tgagaatccg ccggtctgca cttcggtctt cttcacgata ttgacttgtg gcatggacag
3061 caccttccgc acagttgcga agtccctgcc tttatcccac acgatttctc ctgtttctcc
3121 gtttgtttcg atcagtgggc gcttccggat ttcgccgtta gccagggtta tctcagtctt
3181 aaagaaattc atgatattag agtagaagaa gtacttggcg gtggctttgc caatctcttg
3241 ctcagacttt gctatcatct tcctcacatc gtagacttta tagtcaccgt acacgaactc
3301 agactccagt ttagggtatt tcttgatcag ggcggtgcca acgacagcat tgagataggc
3361 atcgtgagca tgatggtaat tgtttatttc gcgaactttg tagaattgaa agtccttccg
3421 gaagtcagac accagcttgc tcttcagagt tatcactttc acttccctga tcagcttatc
3481 gttctcatcg tacttagtgt tcatcctaga gtcgaggatt tgtgccacgt gtttggtaat
3541 ctggcgtgtt tcgaccagtt gcctcttaat aaagccggcc ttgtcgagtt cgctcagacc
3601 tcctctttct gccttagtca gattgtcaaa cttccgctgg gtgatcagtt tggcgttgag
3661 gagctgccgc cagtaattct tcatcttctt gaccacctct tctgatggaa cattgtcaga
3721 cttaccgcga ttcttatcgg atctggtcag caccttgttg tcaatggagt catctttgag
3781 gaaggactgt ggaacaatat ggtccacgtc ataatcggac agccggttga tgtcgagttc
3841 ctggtcaacg tacatgtccc gcccgttctg gaggtagtac aggtagagtt tctcgttctg
3901 gagctgtgta ttctccacag ggtgctcctt cagtatctga gatcccagct ccttaattcc
3961 ctcttcgatt cttttcatcc gttcccgaga gttcttctgg cccttctggg tggtttggtt
4021 ctcccttgcc atttcgataa cgatattctc tggcttgtgc cgacccatca ctttgacgag
4081 ttcgtccacg accttgactg tctgcagtat tcccttcttt atggcgggtg atccagcgag
4141 gttggcgatg tgctcgtgca ggctgtcgcc ttgaccgctc acctgtgcct tctggatgtc
4201 ctctttaaat gtcagagagt catcgtgaat cagttgcatg aagttcctgt tagcgaatcc
4261 gtcggatttc aggaaatcca ggatggtctt tccgctctgt ttgtcgcgga tgccgtttat
4321 gagtttcctg gagagtctac cccacccagt gtagcgccgc cgcttgagct gtttcatgac
4381 tttatcgtca aacagatggg cataggtctt caggcgctct tcgatcatct ctctatcctc
4441 gaacagagtc agggtcagca cgatatcttc caggatgtcc tcgttctcct cattatcgag
4501 gaaatctttg tctttgatta tcttcagcag atcatggtaa gtgcccaggc tggcattaaa
4561 gcggtcctcc acgccagaga tttccactga gtcaaagcat tcgatcttct taaagtaatc
4621 ctccttgagc tgcttcactg tcacctttct attagtcttg aagagcagat caacgatagc
4681 cttcttctgc tctccggaca ggaaggcagg cttcctcatg ccctcggtca cgtacttcac
4741 tttagtcagc tcgttataga cggtgaaata ctcgtacagg agtgaatgtt tgggcaggac
4801 cttctcgttg ggcaggttct tatcgaaatt ggtcatccgt tcgatgaatg actgggcgct
4861 tgcgccctta tccacgacct cttcgaagtt ccaaggagta attgtctcct cagatttcct
4921 ggtcatccaa gcgaagcggg agttgcctct agccagaggg ccgacgtaat aagggatcct
4981 gaaggtcagg atcttctcta tcttctcccg gttatccttc aggaaaggat agaaatcctc
5041 ctgcctgcgg aggattgcat gcagctctcc caggtgtatc tgatgtggaa tggagccatt
5101 atcaaaggtc ctctgcttcc tcagcaggtc ctccctgttc agcttcacca gcagctcttc
5161 agtaccgtcc atcttctcga ggattggttt gatgaacttg taaaattctt cctgtgatgc
5221 tccgccatcg atgtatccgg catatccatt cttgctctgg tcgaagaata tctctttgta
5281 cttctctggc agctgttgcc tcacgagggc tttgagcaga gtcaggtctt gatggtgttc
5341 atcatagcgt tttatcatgg aggcgctcag aggtgctttg gtgatctcag tgttgacccg
5401 gagtatgtcg ctcagcagaa tggcgtcgga gagattctta gcagccagaa agagatcggc
5461 gtactggtcg cctatctgtg cgagcaggtt gtccagatca tcgtcatagg tgtccttgga
5521 gagctggagt ttagcatctt cggccagatc aaaattggac ttgaagttag gtgtcaggcc
5581 caggctcagg gcgatgaggt tcccaaacag gccgttcttc ttttctcctg gcagttgggc
5641 aatcagattc tccagtctgc gtgacttgga cagccgagcg gacagaatag ccttggcatc
5701 cacaccagaa gcgtttatgg gattctcctc gaacagttgg ttatatgtct gcaccagttg
5761 aatgaagagt ttatccacat cggaattatc gggattcagg tcgccctcga tcagaaagtg
5821 tcctctaaac tttatcatat gagccagggc cagatagatg agcctcaggt ctgctttatc
5881 ggtgctgtcc accagcttct tcctcagatg gtagattgta ggatactttt catggtaagc
5941 cacctcatcc acgatatttc cgaatatagg gtgcctctcg tgtttcttat cctcctccac
6001 cagaaagctc tcttccaggc ggtgaaagaa ggagtcgtcc accttagcca tttcgttgct
6061 aaagatctct tgcagataac atatccgatt cttgcggcgg gtgtatctcc gccttgcggt
6121 ccgcttcagc cgagtagctt cagcggtttc accggagtcg aagaggagtg ctccgatcag
6181 gttcttcttg attgaatggc ggtcagtatt acccagcacc ttgaatttct tgcttggcac
6241 cttatactcg tcggttatga cggcccagcc aacggagttt gtcccgatat ccagtccaat
6301 agagtatttc ttgtctctag tgtgggtccg ctggtgtctg gtcagagcac cagactgaga
6361 gaaagattta ccacactctg ggcatttgta tggcttctcg ccgggttcca gtctagattt
6421 atcgtcgtca tccttgtagt cagcggccgc caccttcctc tttttcttag gtcccatggt
6481 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
6541 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
6601 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
6661 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
6721 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
6781 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
6841 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
6901 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
6961 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
7021 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
7081 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
7141 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
7201 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
7261 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
7321 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
7381 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
7441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
7501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
7561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
7621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
7681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
7741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
7801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
7861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
7921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
7981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
8041 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
8101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
8161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
8221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
8281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
8341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
8401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
8461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
8521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
8581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
8641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
8701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
8761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
8821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
8881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
8941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
9001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
9061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
9121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
9181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
9241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
9301 gagcgaggaa gcggaag
//
