| 质粒名称: | pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 71396 |
| 存储实验室: | Fred Gage |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10447 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 2xGFP:NES |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EF1alpha |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI(未破坏) |
| 3' 克隆位点: | -- |
| 5' 测序引物 | EF1a-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS was a gift from Fred Gage (Addgene plasmid # 71396 ; http://n2t.net/addgene:71396 ; RRID:Addgene_71396) |
| 参考文献部分: | Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects. Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y, Winkler J, Yao J, Hetzer MW, Gage FH. Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 10.1016/j.stem.2015.09.001. 10.1016/j.stem.2015.09.001 PubMed 26456686 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS 10447 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 2Gi2R assay.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10447)
AUTHORS Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S,
McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
Winkler J, Yao J, Hetzer MW, Gage FH
TITLE Directly Reprogrammed Human Neurons Retain Aging-Associated
Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic
Defects.
JOURNAL Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi:
10.1016/j.stem.2015.09.001.
PUBMED 26456686
REFERENCE 2 (bases 1 to 10447)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10447
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2283..3460
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2514..3451
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3408..3428
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3488..4204
/codon_start=1
/product="Cycle 3 GFP (Crameri et al., 1996)"
/label=Cycle 3 GFP
/note="mammalian codon-optimized"
/translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(3521..3549)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 4144..4164
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
protein_bind 4226..4250
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4259..4285
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 4295..5014
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4340..4361)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4601..4620)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4948..4969
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5023..5596
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5190..5207)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5417..5436
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5612..5632
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5633..6337
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(5775..5793)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 6043..6062
/label=mCherry-F
/note="mCherry, forward primer"
protein_bind 6365..6389
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
LTR 7165..7798
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7927..7943)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7927..7943)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7940..7962)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7951..7967
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7975..8005)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8020..8041
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8158..8175)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8329..8914)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8409..8428)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9085..9945)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9708..9727
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9946..10050)
/gene="bla"
/label=AmpR promoter
polyA_signal 10098..10232
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10135..10154)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10189..10208
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgcgccc gacgataagc tttgcaaaga tggataaagt
2221 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
2281 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
2341 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
2401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
2461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
2521 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
2581 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
2641 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
2701 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
2761 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
2821 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
2881 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
2941 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
3001 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
3061 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
3121 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
3181 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
3241 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
3301 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
3361 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
3421 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaatttcga cccggatccg
3481 cggccgcatg gccagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
3541 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
3601 tacatacgga aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
3661 gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca
3721 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac
3781 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga
3841 tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
3901 cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca
3961 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca
4021 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
4081 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca
4141 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta
4201 caaaagcggt tccggaccgg atcaaacaag tttgtacaaa aaagcaggct tactacagct
4261 accgccgctg gaaagactga ctctgggagg cgggatggtg agcaagggcg aggagctgtt
4321 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
4381 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
4441 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
4501 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
4561 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
4621 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
4681 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
4741 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
4801 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
4861 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
4921 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
4981 gatcactctc ggcatggacg agctgtacaa gtaggaattc cgcccctctc cctccccccc
5041 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
5101 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
5161 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
5221 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
5281 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
5341 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
5401 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
5461 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
5521 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
5581 aaaaacacga tgataagctt gccgtacgat gccaaaaaag aaaagaaaag ttgtgagcaa
5641 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
5701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
5761 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
5821 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
5881 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
5941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
6001 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
6061 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
6121 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
6181 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
6241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
6301 ccgccactcc accggcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
6361 catcacaagt ttgtacaaaa aagcaggctt aatgagcgag ctgattaagg agaacatgca
6421 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
6481 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
6541 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
6601 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg
6661 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
6721 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
6781 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc
6841 tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg ggggccacct
6901 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
6961 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
7021 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
7081 caaacttaat tagggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
7141 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
7201 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
7261 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
7321 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
7381 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
7441 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
7501 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
7561 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
7801 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
7861 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
7921 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
7981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
8041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
8101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
8161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
8221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
8281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
8341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
8401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
8461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
8521 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
8581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
8641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
8701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
8761 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
8821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
10081 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
10141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
10201 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
10261 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
10321 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
10381 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
10441 tagtagt
//
