| 质粒名称: | pTP1122 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 104159 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pQE80 derivative |
| 载体出品公司: | Clontech |
| 空载体大小: | 4655bp |
| 完整质粒大小: | 4655bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines) |
| 物种来源: | Vicugna pacos |
| 标签/融合蛋白: |
14xHistidine tag (N terminal on backbone)
NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)
|
|
文献引用方法 |
|
| 材料和方法部分: | pTP1122 was a gift from Dirk Görlich (Addgene plasmid # 104159 ; http://n2t.net/addgene:104159 ; RRID:Addgene_104159) |
| 参考文献部分: | A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies. Pleiner T, Bates M, Gorlich D. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. 10.1083/jcb.201709115 PubMed 29263082 |
买家导航
pTP1122载体质粒基本信息
pTP1122质粒图谱载体图谱和pTP1122载体序列质粒序列多克隆位点信息
pTP1122质粒载体简介
pTP1122质粒序列载体序列
LOCUS pTP1122 4654 bp DNA circular SYN 15-DEC-2025
DEFINITION Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107
(3x Cysteines).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4654)
AUTHORS Pleiner T, Bates M, Gorlich D
TITLE A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.
JOURNAL J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi:
10.1083/jcb.201709115. Epub 2017 Dec 20.
PUBMED 29263082
REFERENCE 2 (bases 1 to 4654)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1083/jcb.201709115"; journalName: "J Cell Biol"; date:
"2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1143-1154"
COMMENT Sequence Label: pTP1122
FEATURES Location/Qualifiers
source 1..4654
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter join(1..15,16..21,22..38,39..45)
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 21..37
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 53..69
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 88..99
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 890..984
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
primer_bind complement(1032..1051)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1043..1858
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
CDS 1043..1858
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1116..1135)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 2077..2163
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(2256..3338)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 3300..3319
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(3339..3416)
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
primer_bind complement(3500..3522)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3608..3748
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3663..3682
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3763..3780)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3934..4522)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4014..4033)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4613..4631
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
61 ggataacaat ttcacacaga attcattaaa gaggagaaat taaccatgag caagcatcac
121 catcattcag gccatcacca taccggacac caccatcatt caggcagtca tcaccatacc
181 ggcggatcat ccggaggtat gagctcagga tctaacggat ctacgggttc tggtactggt
241 aatagcggga ccggaaccat gattaaagta aagactctga ccggcaagga aattgagatc
301 gacatcgaac cgaccgacac catcgatcgt atcaaagaac gtgtggagga aaaagaaggc
361 attcctccgg ttcagcagcg tctcatttac gcgggtaaac agcttgcaga tgacaaaacc
421 gccaaagatt acaatatcga gggcggtagc gtactgcacc ttgttctggc cctgcgtggt
481 ggcgccggta cctgcggatc ccaggtgcaa ttggtagagt gtgggggagg cttggtgcag
541 cctggggggt ctctgaggct ctcctgtgca gcctctggat tcacattcag tgatacctgg
601 atgaactggg tccgccaggc tccagggaag gggctctatt ggatctcagc catcaatccg
661 gacggtggta acacagccta tgcagactcc gtcaagggcc ggttcaccat ctccagagac
721 aacgccaaga acatggtgta tctgcagatg gacaacctga gacctgagga cacggccatg
781 tattactgcg caaagggttg ggttcgcctt ccagatcccg atctggtaag gggccagggg
841 acccaggtca cagtctcatc atgttaagct tcttaattag ctgagcttgg actcctgttg
901 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
961 cgggcgtttt ttattggtga gaatccaagc tagccatgaa aataaactgt ctgcttacat
1021 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc
1081 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
1141 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
1201 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
1261 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
1321 tgcttggtta ctcacgactg cgatccccgg caaaacagca ttccaggtat tagaagaata
1381 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
1441 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
1501 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
1561 gcctgttgaa caagtctgga aagaaatgca caaacttttg ccattctcac cggattcagt
1621 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
1681 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
1741 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
1801 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga
1861 attaattcat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
1921 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
1981 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
2041 aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg
2101 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
2161 aatccgccct ctagattacg tgcagtcgat gataagctgt caaacatgag aattgtgcct
2221 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
2281 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
2341 ttgggggcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
2401 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
2461 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
2521 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
2581 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
2641 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
2701 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
2761 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
2821 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
2881 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
2941 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
3001 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
3061 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
3121 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
3181 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
3241 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
3301 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
3361 tcttccgggc gctatcatgc cataccgcga aaggttttgc accattcgat ggtgtcggaa
3421 tttcgggcag cgttgggtcc tggccacggg tgcgcatgat ctagagctgc ctcgcgcgtt
3481 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggcatccgct tacagacaag
3541 ctgtgaccgt ctccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
3601 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
3661 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
3721 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
3781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
3841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
3901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
4381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaca ttaacctata aaaataggcg
4621 tatcacgagg ccctttcgtc ttcacctcga gaaa
//
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