pAAVLINK-3'-ASXL3-FLAG

LOCUS       pAAVLINK-3'-ASXL3-FLAG               6814 bp DNA     circular SYN 19-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     protein_bind    145..178
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          179..270
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             271..3602
                     /codon_start=1
                     /label=ASXL3
                     /translation="GLQT*KSLLPLKQKWKVRLVSLLSIQTVDLLATSLPTSGRPPLYY
                     SSLLTQVNFQKLPLTYLCIVLMKTYLCHIYLRKLFHLPLLKIAVCPCFLIKILSLYLFA
                     ALLYREQLKNIPL*VLLINPLS*CLLTVQTLQFLLVI*AC*KPSRELTLHA*PLYQNVL
                     KALPFQPLQRAPAYQAPWMISSY*YQAAVLVT*SPLSTPLCQLPPSETICQTSPLALS*
                     FPQWELTTDFLLRR*PYLGVKNRPLYPWVPL*EQPSAAVIL*RSQTLWLHTRPSQCLLE
                     TC*Q*TLMIVLPS*VLKAWTKIQGLETGQIILENLSNHQGALHQQP*TDQFRVKSSLTT
                     APR*PPVCL*LSPLKAQKPAWTCRADQ*GQRHPYSPWRVLRCL*LAGLSQLPTKV*ITV
                     PLSLLLRQQNKTVKHCRPPAQVSENYPLFQIN*MSRLLPVITLLSRHVAQLLSKVKQTQ
                     PVAISITQVTGFAGMMMG*GAQDSLWLLTRVQVNKKNI*SKAVQRLSKLNMPTT*TCQN
                     FIPGIL*QMLLFL*NLNFTKQTRALEWTLKTSLALSCLLRLQREPLVYNKHRT*KLPPQ
                     VPWKRLFPWLPMP*RESLVQGAQAVVCPLWRLTIRW*RSYYRATCLWKKCCHSPDWEPS
                     LKSTGFHCLFKLPQWVKQHQRETLKFRPALQIQMVRATWQGLWHHSK*ESEKTTPKRE*
                     LGL*ENTLKLNVNQENCWWSQMLKGCLVSSVPASVS*DTASHLSKNG*TSTPCRTELFT
                     ALRSNSKSGCSPRVASSRTYFMLTRMAASTLTLVPHTDSSFTKCLWLPGAPFLLQLCYR
                     PLPRPQWGVMHLPSTGILNRRDWERLVFPQHLTS*G*PTCYPPICP*KKVMRWEALHTQ
                     CQTKH*YIRRRHRLPLPLHPWLCPRLPPHHLRYLHLSLMQKSHLIKNNLQLPWKPLRDL
                     VGHSPRAYVAI*NRNLFLLRKV*AAAVNWA*NKFPMTRMK*KNS*KHSR*KVQISLPIC
                     FLSHKSLLPN*LLRKCRCSNNSSSVEIIQQYTLVARVSKGQHLQLKSPLGFWEVAPILP
                     QACLVRTLRCPFRTLPTAAMQMNWN*NALAG*KP*LCAKAVGPSAMTTA*VLQNFV*HA
                     WLY"
     misc_feature    1932..1937
                     /label=SDS
     CDS             3609..3674
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3678..4060
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3822..3827
                     /label=SDS
     repeat_region   4077..4217
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4077..4206
                     /label=AAV2 ITR
     rep_origin      4292..4747
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5029..5133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5134..5994
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6165..6753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
      181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      241 ctgacatcca ctttgccttt ctctccacag ggcctccaaa cttagaagtc tcttctaccc
      301 ctgaaacaaa aatggaaggt tcgactggtg tcattattgt caatccaaac tgtagatctc
      361 ctagcaacaa gtctgcccac ctccgggaga ccaccactgt actacagcag tctcttaacc
      421 caagtaaact tccagaaact gccactgact tatctgtgca tagttctgat gaaaacatac
      481 ctgtgtcaca tttatctgag aaaattgttt catctacctc ttctgaaaat agcagtgtgc
      541 ccatgctttt taataaaaat tctgtccctg tatctgtttg cagcactgct atatcgggag
      601 caattaaaga acatcccttt gtgagttctg ttgataaatc ctctgtccta atgtctgttg
      661 acagtgcaaa cactacaatt tctgcttgta atataagcat gttaaaaacc atccagggaa
      721 ctgacactcc atgcatagcc attataccaa aatgtattga aagcactccc atttcagcca
      781 ctacagaggg ctccagcata tcaagctcca tggatgataa gcagttacta atatcaagca
      841 gcagtgctag taacttagtc tccactcagt acacctctgt gccaactccc tccatcggaa
      901 acaatttgcc aaacctctcc actagctctg tcttgattcc cccaatggga attaacaaca
      961 gatttccttc tgagaagata gccatacctg ggagtgaaga acaggccact gtatccatgg
     1021 gtaccactgt gagagcagcc ctcagctgca gtgattctgt agcggtcaca gactctctgg
     1081 ttgcacaccc gaccgtcgca atgtttactg gaaacatgct gacaataaac tcttatgata
     1141 gtcctcccaa gttaagtgct gaaagcttgg acaaaaattc agggcctcga aacagggcag
     1201 ataattctgg aaaacctcag caaccaccag ggggctttgc accagcagcc ataaaccgat
     1261 caattccgtg taaagtcatc gttgaccaca gcaccacgct gacctccagt ttgtctctga
     1321 ctgtctccgt tgaaagctca gaagccagct tggacctgca gggcagacca gtgaggacag
     1381 aggcatccgt acagcccgtg gcgtgtcctc aggtgtctgt gattagcagg cctgagccag
     1441 ttgccaacga aggtatagat cacagttcca ctttcattgc tgcttcggca gcaaaacaag
     1501 acagtaaaac attgccggcc acctgcacaa gtctccgaga attacccctt gttccagata
     1561 aattaaatga gccgactgct cccagtcata actttgctga gcaggcacgt ggcccagctc
     1621 ctttcaaaag tgaagcagac acaacctgta gcaatcagta taacccaagt aaccggattt
     1681 gctggaatga tgatgggatg aggagcacag gacagcctct ggttactcac tcgggttcaa
     1741 gtaaacaaaa agaatatcta gagcaaagct gtccaaaggc tatcaaaact gaacatgcca
     1801 actacttgaa cgtgtcagaa cttcatccca ggaatcttgt aacaaatgtt gctcttcctg
     1861 tgaaatctga acttcacgaa gcagacaagg gctttagaat ggacactgaa gacttccctg
     1921 gccctgagct gcctcctccg gctgcagagg gagcctctag tgtacaacaa acacagaaca
     1981 tgaaagcttc cacctcaagt cccatggaag aggctatttc cttggctacc gatgccctga
     2041 agagagtccc tggtgcaggg agctcaggct gtcgtctgtc ctctgtggag gctaacaatc
     2101 cgctggtgac gcagttacta cagggcaacc tgcctttgga aaaagtgttg ccacagccca
     2161 gattgggagc caagcttgaa atcaacaggc ttccattgcc tcttcaaact acctcagtgg
     2221 gtaaaacagc accagagaga aacgttgaaa ttccgcccag ctctccaaat ccagatggta
     2281 agggctactt ggcagggact ctggcaccac tccaaatgag aaagcgagaa aaccacccca
     2341 aaaagagagt agctaggact gtaggagaac acactcaagt taaatgtgaa ccaggaaaat
     2401 tgttggtgga gccagatgtt aaaggggtgc cttgtgtcat cagttccggc atcagtcagc
     2461 taggacacag ccagccattt aagcaagaat ggctaaacaa gcactccatg cagaacagaa
     2521 ttgttcacag ccctgaggtc aaacagcaaa agcggctgct cccctcgtgt agcttccagc
     2581 agaacctatt tcatgttgac aagaatggcg gcttccacac tgacgctggt acctcacaca
     2641 gacagcagtt ttaccaaatg cctgtggctg ccaggggccc cattcctact gcagctctgt
     2701 tacaggcctc ttccaagacc ccagtggggt gtaatgcatt tgccttcaac aggcatcttg
     2761 aacagaaggg attgggagag gttagtcttt cctcagcacc tcaccagcta aggttagcca
     2821 acatgttatc ccccaatatg cccatgaaag aaggtgatga ggtgggaggc actgcacaca
     2881 caatgccaaa caaagcacta gtacatccgc cgccgccacc gcctccccct ccccctccac
     2941 ccttggcttt gcccccgcct ccccccccac cacctccgct acctccacct ctccctaatg
     3001 cagaagtccc atctgatcaa aaacaacctc cagttaccat ggaaaccact aagagactta
     3061 gttggccaca gtccacgggc atatgtagca atataaaatc ggaacctctt tcttttgagg
     3121 aaggtttaag cagcagctgt gaactgggca tgaaacaagt ttcctatgac cagaatgaaa
     3181 tgaaagaaca gttaaaagca ttcgcgctaa aaagtgcaga tttctcttcc tatttgcttt
     3241 ctgagccaca aaagcctttt acccaattag ctgctcagaa aatgcaggtg cagcaacaac
     3301 agcagctctg tggaaattat ccaacaatac actttggtag cacgagtttc aaaagggcag
     3361 catctgcaat tgaaaagtcc attgggattt tgggaagtgg ctccaatcct gccacaggct
     3421 tgtctggtca gaacgctcag atgcccgttc agaactttgc cgacagcagc aatgcagatg
     3481 aattggaact gaaatgctct tgccggctga aagccatgat tgtgtgcaaa ggctgtgggg
     3541 ccttctgcca tgacgactgc ataggtcctt caaaactttg tgtagcatgc ctggttgtac
     3601 gatgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     3661 atgacgatga caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt
     3721 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3781 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3841 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3901 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
     3961 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4021 tatgtttcag gttcaggggg agatgtggga ggttttttaa cggaccgagc ggccgcagga
     4081 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4141 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4201 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4261 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4321 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4381 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4441 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4501 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4561 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4621 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     4681 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4741 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4801 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4861 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4921 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4981 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5041 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5101 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5161 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5221 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5281 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5341 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5401 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5461 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5521 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5581 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5641 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5701 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5761 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5821 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5881 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5941 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6001 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6061 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6121 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6181 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6241 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6301 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6361 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6421 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6481 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6541 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6601 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6661 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6721 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6781 ttcctggcct tttgctggcc ttttgctcac atgt
//