| 质粒名称: | pAAVLINK-EF1a-HA-5'-BLM |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001674 |
| 产品用途: | 5' AAVLINK plasmid for BLM expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BLM |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-BLM
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-BLM载体质粒基本信息
pAAVLINK-EF1a-HA-5'-BLM质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-BLM载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-BLM质粒载体简介
pAAVLINK-EF1a-HA-5'-BLM质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-BLM 6279 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6279)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6279
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3419
/codon_start=1
/label=BLM
/translation="MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDN
NVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGS
KSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTS
ETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTE
EQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELH
STEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKD
LLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNE
LLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHL
PSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNES
SYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAA
SKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLA
SRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGG
KSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQL
SKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYK
RMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKP
KKVAFDCLEWIRKHHPYDS"
misc_feature 1380..1385
/label=SDS
intron 3420..3460
/label=Splicing Donor
misc_feature 3464..3497
/label=lox2272-JT1571
repeat_region 3542..3682
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3542..3671
/label=AAV2 ITR
rep_origin 3757..4212
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4494..4598
/gene="bla"
/label=AmpR promoter
CDS 4599..5459
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5630..6218
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggctgctgtt cctcaaaata atctacagga
781 gcaactagaa cgtcactcag ccagaacact taataataaa ttaagtcttt caaaaccaaa
841 attttcaggt ttcactttta aaaagaaaac atcttcagat aacaatgtat ctgtaactaa
901 tgtgtcagta gcaaaaacac ctgtattaag aaataaagat gttaatgtta ccgaagactt
961 ttccttcagt gaacctctac ccaacaccac aaatcagcaa agggtcaagg acttctttaa
1021 aaatgctcca gcaggacagg aaacacagag aggtggatca aaatcattat tgccagattt
1081 cttgcagact ccgaaggaag ttgtatgcac tacccaaaac acaccaactg taaagaaatc
1141 ccgggatact gctctcaaga aattagaatt tagttcttca ccagattctt taagtaccat
1201 caatgattgg gatgatatgg atgactttga tacttctgag acttcaaaat catttgttac
1261 accaccccaa agtcactttg taagagtaag cactgctcag aaatcaaaaa agggtaagag
1321 aaactttttt aaagcacagc tttatacaac aaacacagta aagactgatt tgcctccacc
1381 ctcctctgaa agcgagcaaa tagatttgac tgaggaacag aaggatgact cagaatggtt
1441 aagcagcgat gtgatttgca tcgatgatgg ccccattgct gaagtgcata taaatgaaga
1501 tgctcaggaa agtgactctc tgaaaactca tttggaagat gaaagagata atagcgaaaa
1561 gaagaagaat ttggaagaag ctgaattaca ttcaactgag aaagttccat gtattgaatt
1621 tgatgatgat gattatgata cggattttgt tccaccttct ccagaagaaa ttatttctgc
1681 ttcttcttcc tcttcaaaat gccttagtac gttaaaggac cttgacacct ctgacagaaa
1741 agaggatgtt cttagcacat caaaagatct tttgtcaaaa cctgagaaaa tgagtatgca
1801 ggagctgaat ccagaaacca gcacagactg tgacgctaga cagataagtt tacagcagca
1861 gcttattcat gtgatggagc acatctgtaa attaattgat actattcctg atgataaact
1921 gaaacttttg gattgtggga acgaactgct tcagcagcgg aacataagaa ggaaacttct
1981 aacggaagta gattttaata aaagtgatgc cagtcttctt ggctcattgt ggagatacag
2041 gcctgattca cttgatggcc ctatggaggg tgattcctgc cctacaggga attctatgaa
2101 ggagttaaat ttttcacacc ttccctcaaa ttctgtttct cctggggact gtttactgac
2161 taccacccta ggaaagacag gattctctgc caccaggaag aatctttttg aaaggccttt
2221 attcaatacc catttacaga agtcctttgt aagtagcaac tgggctgaaa caccaagact
2281 aggaaaaaaa aatgaaagct cttatttccc aggaaatgtt ctcacaagca ctgctgtgaa
2341 agatcagaat aaacatactg cttcaataaa tgacttagaa agagaaaccc aaccttccta
2401 tgatattgat aattttgaca tagatgactt tgatgatgat gatgactggg aagacataat
2461 gcataattta gcagccagca aatcttccac agctgcctat caacccatca aggaaggtcg
2521 gccaattaaa tcagtatcag aaagactttc ctcagccaag acagactgtc ttccagtgtc
2581 atctactgct caaaatataa acttctcaga gtcaattcag aattatactg acaagtcagc
2641 acaaaattta gcatccagaa atctgaaaca tgagcgtttc caaagtctta gttttcctca
2701 tacaaaggaa atgatgaaga tttttcataa aaaatttggc ctgcataatt ttagaactaa
2761 tcagctagag gcgatcaatg ctgcactgct tggtgaagac tgttttatcc tgatgccgac
2821 tggaggtggt aagagtttgt gttaccagct ccctgcctgt gtttctcctg gggtcactgt
2881 tgtcatttct cccttgagat cacttatcgt agatcaagtc caaaagctga cttccttgga
2941 tattccagct acatatctga caggtgataa gactgactca gaagctacaa atatttacct
3001 ccagttatca aaaaaagacc caatcataaa acttctatat gtcactccag aaaagatctg
3061 tgcaagtaac agactcattt ctactctgga gaatctctat gagaggaagc tcttggcacg
3121 ttttgttatt gatgaagcac attgtgtcag tcagtgggga catgattttc gtcaagatta
3181 caaaagaatg aatatgcttc gccagaagtt tccttctgtt ccggtgatgg ctcttacggc
3241 cacagctaat cccagggtac agaaggacat cctgactcag ctgaagattc tcagacctca
3301 ggtgtttagc atgagcttta acagacataa tctgaaatac tatgtattac cgaaaaagcc
3361 taaaaaggtg gcatttgatt gcctagaatg gatcagaaag caccacccat atgattcagg
3421 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat
3481 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
3541 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
3601 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
3661 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
3721 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
3781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
3841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3961 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4021 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
4081 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
4141 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
4201 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
4261 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
4321 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
4381 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
4441 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
5041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
5101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
5161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
5221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5281 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5761 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
6001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
6061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
6121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
6181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6241 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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pAAVLINK-EF1a-HA-5'-BLM质粒载体应用举例
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