pBudCE4.1

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司:
Invitrogen
载体名称: pBudCE4.1
质粒类型: 哺乳动物细胞表达载体
高拷贝/低拷贝: --
启动子: CMV or EF-1a
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4595 bp
5' 测序引物及序列: CMV Forward: 5'd[TAATACGACTCACTATAGGG]3'
EF-1a Forward:5'd[TCAAGCCTCAGACAGTGGTTC]3'
3' 测序引物及序列: --
载体标签: 6XHis; c-myc epitope
载体抗性: zeocin
筛选标记: --
备注: 克隆宿主菌E.coli TOP10 ;DH5alpha T1 。
该载体能够独立表达两种基因,是双表达载体。表达水平高。
产品目录号: V86420
稳定性: --
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pBudCE4.1载体图谱



pBudCE4.1多克隆位点

pBudCE4.1载体特征

载体简介

pBudCE4.1 载体使用时的注意事项:

     现在市面上出售的pBudCE4.1载体,在多克隆位点位置都有突变,我们保证我们载体质粒序列的正确性,请大家放心购买!

     1.在使用E.coli 克隆菌株时最好使用不含Tn5基因的菌株,如TOP10和DH5α T1;

     2.采用电转法时最好用TOP10;

     3.Zeocin 工作浓度推荐25 mg/L ~ 50 mg/L

载体序列

LOCUS       pBudCE4.1	4595 bp 	DNA	SYN
DEFINITION  pBudCE4.1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="pBudCE4.1"
                     /mol_type="other DNA"
     promoter        11..627
                     /label="CMV_immearly_promoter"
     misc_feature    90..377
                     /label="CAG_enhancer"
     misc_feature    544..564
                     /label="CMV_fwd_primer"
     promoter        638..656
                     /label="T7_promoter"
     misc_feature    complement(823..842)
                     /label="EBV_rev_primer"
     misc_feature    complement(1063..1437)
                     /label="sh_ble"
     gene            complement(1066..1437)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(1438..1505)
                     /label="EM7_promoter"
     misc_feature    complement(1619..1638)
                     /label="SV40pro_F_primer"
     rep_origin      complement(1623..1700)
                     /label="SV40_origin"
     misc_feature    1670..1861
                     /label="SV40_enhancer"
     CDS             complement(1877..2791)
                     /label="ORF frame 2"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     promoter        1884..3051
                     /label="EF1a_promoter"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     misc_feature    2999..3019
                     /label="EF1a_fwd_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     misc_feature    complement(3218..3235)
                     /label="BGH_rev_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     terminator      3221..3448
                     /label="bGH_PA_terminator"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     rep_origin      complement(3565..4184)
                     /label="pBR322_origin"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
     misc_feature    complement(4315..4337)
                     /label="pGEX_3_primer"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
                     IADPSPQLLGDCGRCALCPLTGTGASRS*"
ORIGIN
    1 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
   61 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
  121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
  181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG
  241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
  301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
  361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
  421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
  481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
  541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
  601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA
  661 CCCAAGCTTG CATTCCTGCA GGTCGACATC GATCTTAAGC AGTACTTCTA GAGGATCCGA
  721 ACAAAAACTC ATCTCAGAAG AGGATCTGAA TATGCATACC GGTCATCATC ACCATCACCA
  781 TTGAGTTTGA TCCCCGGGAA TTCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
  841 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  901 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTGGGGTGG GCGAAGAACT CCAGCATGAG
  961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT
 1021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTAGCACG TGTCAGTCCT GCTCCTCGGC
 1081 CACGAAGTGC ACGCAGTTGC CGGCCGGGTC GCGCAGGGCG AACTCCCGCC CCCACGGCTG
 1141 CTCGCCGATC TCGGTCATGG CCGGCCCGGA GGCGTCCCGG AAGTTCGTGG ACACGACCTC
 1201 CGACCACTCG GCGTACAGCT CGTCCAGGCC GCGCACCCAC ACCCAGGCCA GGGTGTTGTC
 1261 CGGCACCACC TGGTCCTGGA CCGCGCTGAT GAACAGGGTC ACGTCGTCCC GGACCACACC
 1321 GGCGAAGTCG TCCTCCACGA AGTCCCGGGA GAACCCGAGC CGGTCGGTCC AGAACTCGAC
 1381 CGCTCCGGCG ACGTCGCGCG CGGTGAGCAC CGGAACGGCA CTGGTCAACT TGGCCATGGT
 1441 TTAGTTCCTC ACCTTGTCGT ATTATACTAT GCCGATATAC TATGCCGATG ATTAATTGTC
 1501 AACACGTGCT GATCAGATCC GAAAATGGAT ATACAAGCTC CCGGGAGCTT TTTGCAAAAG
 1561 CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC AGAGGCGGCC
 1621 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
 1681 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG
 1741 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT
 1801 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT
 1861 CCACACCCTC GTCGAGCTAG CTTCGTGAGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC
 1921 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG
 1981 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA
 2041 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG
 2101 GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC
 2161 GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTCCAGTACG TGATTCTTGA
 2221 TCCCGAGCTG GAGCCAGGGG CGGGCCTTGC GCTTTAGGAG CCCCTTCGCC TCGTGCTTGA
 2281 GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC
 2341 TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG
 2401 CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAGG ATCTGCACAC TGGTATTTCG
 2461 GTTTTTGGGC CCGCGGCCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG
 2521 CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT
 2581 GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC
 2641 CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TCCAGGGGGC
 2701 TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA
 2761 AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC
 2821 AGGCACCTCG ATTAGTTCTG GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG
 2881 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG
 2941 CACTTGATGT AATTCTCGTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC
 3001 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGTGGT
 3061 CGCGGCCGCT TCGAAGGTAC CAGCACAGTG GACTCGAGAG ATCTGGCCGG CTGGGCCCGT
 3121 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
 3181 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG TGGCGGTAAT
 3481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
 3541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
 3601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
 3661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
 3721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
 3781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
 3841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
 3901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
 3961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
 4021 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
 4081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
 4141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
 4201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACATTAA CCTATAAAAA
 4261 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
 4321 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
 4381 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC
 4441 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC
 4501 GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA
 4561 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCA
//

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