| 质粒名称: | pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74928 |
| 存储实验室: | Marvin Tanenbaum |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4TO |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 博来霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 24xGCN4_v4-kif18b-24xPP7 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 was a gift from Marvin Tanenbaum (Addgene plasmid # 74928 ; http://n2t.net/addgene:74928 ; RRID:Addgene_74928) |
| 参考文献部分: | Dynamics of Translation of Single mRNA Molecules In Vivo. Yan X, Hoek TA, Vale RD, Tanenbaum ME. Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034. 10.1016/j.cell.2016.04.034 PubMed 27153498 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 10960 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Translation reporter.
ACCESSION .
VERSION .
KEYWORDS pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10960)
AUTHORS Yan X, Hoek TA, Vale RD, Tanenbaum ME
TITLE Dynamics of Translation of Single mRNA Molecules In Vivo.
JOURNAL Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
PUBMED 27153498
REFERENCE 2 (bases 1 to 10960)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10960
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 841..859
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 869..893
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 984..993
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1059..1115
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1131..1187
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1203..1259
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1275..1331
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1347..1403
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1419..1475
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1491..1547
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1563..1619
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1635..1691
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1707..1763
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1779..1835
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1851..1907
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1923..1979
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1995..2051
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2067..2123
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2139..2195
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2211..2267
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2283..2339
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2355..2411
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2427..2483
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2499..2555
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2571..2627
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2715..2771
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
primer_bind complement(6971..6988)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6977..7201
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7247..7675
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7334..7353)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7544..7565
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7684..7704)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7689..8018
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7869..8004
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7931..7950
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8066..8113
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8132..8506
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8636..8757
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8673..8692)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8727..8746
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8806..8822)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8806..8822)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8819..8841)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8830..8846
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8854..8884)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8899..8920
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9037..9054)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9208..9793)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9288..9307)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9964..10824)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10587..10606
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10825..10929)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcagtg gaggacagca
2821 cgctgcaagt agtggtacgg gtgcggcccc ccacccctcg ggagctggac agtcagcggc
2881 ggccagtggt tcaggtggtg gacgagcggg tgctggtgtt taaccctgag gagcccgatg
2941 gagggttccc tggcctgaaa tggggtggca cccatgatgg ccccaagaag aagggcaaag
3001 acctgacgtt tgtctttgac cgggtctttg gcgaggcggc cacccaacag gacgtgttcc
3061 agcacaccac gcacagcgtc ctggacagct tcctccaggg ctacaactgc tcagtgtttg
3121 cctacggggc caccggggct gggaagacac acaccatgct gggaagggag ggggaccccg
3181 gcatcatgta cctgaccacc gtggaactgt acaggcgcct ggaggcccgc cagcaggaga
3241 agcacttcga ggtgctcatc agctaccagg aggtgtataa tgaacagatc catgacctcc
3301 tggagcccaa ggggcccctt gccatccgcg aggaccccga caagggggtg gtggtgcaag
3361 gactttcttt ccaccagcca gcctcagccg agcagctgct ggagatactg accaggggga
3421 accgtaaccg cacgcagcac cccactgatg ccaacgcgac ttcctcccgc tcccatgcca
3481 tcttccagat ctttgtgaag cagcaggacc gggttccagg actgacccag gctgtccagg
3541 tggccaagat gagcctgatt gacctggctg gctcagagcg ggcatccagc acccatgcga
3601 agggggagcg gctgcgggag ggggccaaca tcaaccgctc tctgctggcg ctcatcaacg
3661 tcctcaatgc cttggccgat gcaaagggcc gcaagaccgc tgtgccctac gcggacagcg
3721 cactgacccg cctgctcaaa gactccctcg ggggcaactg ccgcacagtg atgatcgctg
3781 ccatcagccc ctccagcctg acctacgagg acacgtataa taccctcaaa tatgccgacc
3841 gggccaagga gatcaggctc tcgctgaaga gcaatgtgac cagcctggac tgtcacatca
3901 gccagtatgc taccatctgc caacagctcc aggctgaggt agccgctctg aggaagaagc
3961 tccaagtgta tgagggggga ggccagcccc caccacagga cctcccagga tctcccaagt
4021 cgggaccacc accagaacac cttcccagct cccccttgcc accccaccct cccagccagc
4081 cctgcacccc agagctccct gcagggccta gagcccttca agaggagagt ctggggatgg
4141 aggcccaggt ggagagggcc atggaaggga actcttcaga ccaggagcag tccccagagg
4201 atgaggatga aggcccagct gaggaggttc caacccagat gccagagcag aaccccacac
4261 atgcactgcc agagtcccct cgcctgaccc tgcagcccaa gccagtcgtg ggccacttct
4321 cagcacggga actggatggg gaccgttcta agcggttggc cctaaaggtg ctgtgcgttg
4381 cccagcggca gtactccctg ctccaagcag ccaacctcct gacgcccgac atgatcacag
4441 agtttgagac cctacagcag ctggtgcaag aggaaaaaat tgagcctggg gcagaggcct
4501 tgaggacttc aggcctggcc aggggggcac ctctggctca ggagctgtgt tcagagtcaa
4561 agcctccagg atacactggc cctgtgaccc ggactatggc gaggcgactg agtggccccc
4621 tgcacaccct gggaatcccg cctggaccca actgcacccc agcccagggg tcccgatggc
4681 ccatggagaa gaagaggagg agaccaagcg ccttggaggc agacagtccc atggccccaa
4741 agcggggcac caagcgccag cgccagtcct tcctgccctg cctaaggaga gggtctctgc
4801 ctgacaccca accttcacag gggcccagca cccccaaagg agaaagggcc tcctccccct
4861 gccattcccc tcgcgtttgc ccagccacag tcatcaaaag ccgggtgccc ctgggccctt
4921 ccgccatgca gaactgctcc accccgctgg ctctgcccac tcgagacctc aatgccacct
4981 ttgatctctc tgaggagcct ccctcaaagc ccagtttcca tgaatgcatt ggctgggaca
5041 aaatacccca ggagctgagc aggctggacc agcccttcat ccccagggca cctgtgcccc
5101 tgttcaccat gaagggcccc aagccaacat cttccctccc tgggacctct gcctgcaaga
5161 agaagcgcgt tgcgagttcc tcagtctccc atggccgcag ccgcatcgcc cgcctcccca
5221 gcagcacttt gaagaggcca gctgggcccc ttgtactccc agagctgccc ttgagtcccc
5281 tgtgccctag caaccggagg aatggaaagg acctcatcag ggtggggaga gcactctcag
5341 cagggaacgg cgtcaccaag gtgtccgata tctaacgaca cgattcgaag atcgcatcgc
5401 tagcccggtt gtacaggtac cggatcctaa ggtacctaat tgcctagaaa ggagcagacg
5461 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
5521 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
5581 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
5641 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
5701 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
5761 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
5821 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
5881 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
5941 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
6001 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
6061 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
6121 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
6181 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
6241 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
6301 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
6361 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
6421 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
6481 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
6541 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
6601 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
6661 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
6721 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
6781 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
6841 gggctcgctg gctgcagtat tcccgggttc attagatctc gcgaagggcg aattcacgag
6901 gcgcgcccgg atcgcgatcg caatcttaat taactcgagt ctagagggcc cgtttaaacc
6961 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
7021 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
7081 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
7141 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
7201 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
7261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
7321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
7381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
7441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
7501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
7561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
7621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
7681 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
7741 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
7801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
7861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
7921 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
7981 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
8041 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
8101 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
8161 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
8221 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
8281 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
8341 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
8401 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
8461 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
8521 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
8581 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
8641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
8701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
8761 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
8821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
8881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
8941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
9001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
9061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
9121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
9181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
9241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
9301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
9361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
9421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
9481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
9541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
9601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
9661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
9721 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
9781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
9841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
9901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
9961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
10021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
10081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
10141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
10201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
10261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
10321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
10381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
10441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
10501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
10741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
10801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
10861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
10921 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
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