pMN20

LOCUS       pMN20               11073 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMN20
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, 
            Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, 
            Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, 
            Yanofsky MF, Chory J
  TITLE     Activation tagging in Arabidopsis.
  JOURNAL   Plant Physiol. 2000 Apr . 122(4):1003-13.
  PUBMED    10759496
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pMN20|
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(60..76)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(61..77)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2053..2069)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2053..2069)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2109..2125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2109..2125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2122..2144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2133..2149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2157..2187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2202..2223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2388..2412
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3712..4341
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4775..5842
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5908..6102
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6338..6360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6446..6586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6501..6520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6601..6618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6852..6871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7604..8395)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8923..8947
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9045..9219
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9273..10070)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9384..9403)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9994..10013
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10130..10808)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(10173..10193)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     11022..11044
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11036..11053
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11037..11053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtcgactct agaggatccc cgggtaccga gctcgagatc tagatatcga taagcttatc
       61 gataccgtcg acctcgaggg gccaacagag cctggcgttc cccttttgca ttgagaccga
      121 tgttcgttcc ggaaccttgc acgccccaga gcttctcacc gttcacgaca atttccttct
      181 cgttgaggtc ggtcgcgcca tgtcggatga aataaaaact tttgatacca gcgggggcct
      241 tcgcagagcc gaggtaggtc tgagaaattg gcattttcac gtgtggaaga tatgaatttt
      301 tttgagaaac tagataagat taatgaatat cggtgttttg gttttttctt gtggccgtct
      361 ttgtttatat tgagattttt caaatcagtg cgcaagacgt gacgtaagta tccgagtcag
      421 tttttatttt tctactaatt tggtcgttta tttcggcgtg taggacatgg caaccgggcc
      481 tgaatttcgc gggtattctg tttctattcc aactttttct tgatccgcag ccattaacga
      541 cttttgaata gatacgctga cacgccaagc ctcgctattc aaaagtgtac caaacaacgc
      601 tttacagcaa gaacggaatg cgcgtgacgc tcgcggtgac gccatttcgc cttttcagaa
      661 atggataaat agccttctca aacactgata gtttcggatc tagatatcac atcaatccac
      721 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg
      781 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca
      841 atgatggcat ttgtagaagc catcttcctt ttctactgtc ctttcgatga agtgacagat
      901 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
      961 agccctctgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     1021 ctccaccatg ttggggatct agatatcaca tcaatccact tgctttgaag acgtggttgg
     1081 aacgtcttct ttttccacga tgttcctcgt gggtgggggt ccatctttgg gaccactgtc
     1141 ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtagaagcc
     1201 atcttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag
     1261 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctctggt cttctgagac
     1321 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggggatcta
     1381 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     1441 gttcctcgtg ggtgggggtc catctttggg accactgtcg gtagaggcat cttgaacgat
     1501 agcctttcct ttatcgcaat gatggcattt gtagaagcca tcttcctttt ctactgtcct
     1561 ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     1621 ttgttgaaaa gtctcaatag ccctctggtc ttctgagact gtatctttga tattcttgga
     1681 gtagacgaga gtgtcgtgct ccaccatgtt ggggatctag atatcacatc aatccacttg
     1741 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ttcctcgtgg gtgggggtcc
     1801 atctttggga ccactgtcgg tagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     1861 atggcatttg tagaagccat cttccttttc tactgtcctt tcgatgaagt gacagatagc
     1921 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     1981 cctctggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     2041 caccatgttg gggatccact agttctagag cggccgccac cgcggtggag ctcgaattcg
     2101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgagc
     2341 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     2401 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     2461 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     2521 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     2581 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     2641 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     2701 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     2761 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     2821 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     2881 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     2941 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     3001 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     3061 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     3121 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     3181 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     3241 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     3301 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     3361 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     3421 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     3481 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     3541 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     3601 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     3661 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     3721 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     3781 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     3841 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     3901 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     3961 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     4021 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     4081 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     4141 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     4201 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     4261 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     4321 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     4381 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     4441 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     4501 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     4561 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     4621 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     4681 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     4741 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     4801 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     4861 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     4921 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     4981 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     5041 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     5101 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     5161 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     5221 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     5281 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     5341 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     5401 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     5461 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     5521 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     5581 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     5641 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     5701 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     5761 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     5821 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     5881 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     5941 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     6001 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     6061 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     6121 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     6181 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     6241 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     6301 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6481 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6541 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7201 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga
     7441 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt
     7501 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac
     7561 gccggcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct
     7621 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     7681 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     7741 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     7801 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     7861 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     7921 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     7981 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     8041 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     8101 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     8161 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     8221 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     8281 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     8341 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt
     8401 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     8461 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     8521 caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     8581 cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct
     8641 tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaattgatca caggcagcaa
     8701 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     8761 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     8821 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     8881 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     8941 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
     9001 aattaacgcc gaattaattc gggggatcta tagggacttt aggtgatctg gattttagta
     9061 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     9121 acatactaag ggtttcttat atgctcaaca cgtgagcgaa accctataag aaccctaatt
     9181 cccttatctg ggaactactc acacattatt atggagaaac tcgatgtcga tcgactctag
     9241 ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     9301 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     9361 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     9421 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     9481 atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc gggcatgcgc
     9541 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     9601 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     9661 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     9721 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     9781 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     9841 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag ttcattcagg
     9901 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     9961 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
    10021 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat ggtcgatcga
    10081 cagatctgcg aatcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc
    10141 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
    10201 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
    10261 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
    10321 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
    10381 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
    10441 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
    10501 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac
    10561 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
    10621 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
    10681 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
    10741 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
    10801 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
    10861 gagtgtcgtg ctccaccatg ttggcaagct gctctagcat tcgccattca ggctgcgcaa
    10921 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10981 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11041 aacgacggcc agtgccaagc ttgcatgcct gca
//