pEarleyGate104

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pEarleyGate104; pEarleyGate 104
质粒类型: Gateway载体;植物双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway
启动子: 35S
载体大小: 12505 bp
5' 测序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: 黄色荧光蛋白EYFP(N ter)
载体抗性: 卡那霉素、氯霉素(仅空载体)
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、
克隆菌株: DB3.1菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEarleyGate104载体图谱



载体简介

载体序列

LOCUS       Exported               12505 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible plant transformation vector with a YFP 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    pEarleyGate 104
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 8613.
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 
                     乙酰转移酶"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /note="35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2681..2735
                     /note="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2737..3450
                     /codon_start=1
                     /product="enhanced YFP"
                     /note="EYFP"
                     /note="mammalian codon-optimized"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     protein_bind    3520..3639
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3669..3699
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3753..4412
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4754..5059
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5100..5224)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5276..5983
                     /note="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    6227..6251
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7551..8180
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8609..9682
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9748..9942
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10286..10426
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10612..11200)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11287..12081)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgg gcaagggcga ggagctgttc
     2761 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2821 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2881 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2941 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3001 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3061 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3121 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3181 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     3241 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     3361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3421 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     3481 aattctgcag tcgacggtac cgcgggcccg ggatcatcaa caagtttgta caaaaaagct
     3541 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     3601 cagactacat aatactgtaa aacacaacat atccagtcat attggcggcc gcattaggca
     3661 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     3721 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     3781 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     3841 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     3901 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     3961 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     4021 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     4081 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     4141 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     4201 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     4261 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     4321 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     4381 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
     4441 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     4501 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     4561 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     4621 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     4681 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     4741 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     4801 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     4861 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     4921 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     4981 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     5041 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     5101 atagtgactg gatatgttgt gttttacagc attatgtagt ctgtttttta tgcaaaatct
     5161 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     5221 tggtgctagg tgagtctaga gagttaatta agacccggga ctagtcccta gagtcctgct
     5281 ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca
     5341 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct
     5401 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac
     5461 tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata ttgtgctgaa
     5521 taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc gttttattat
     5581 tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac tgattacata
     5641 aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca ccgagcggcg
     5701 aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg ggtgagattc
     5761 cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat tcaacccggt
     5821 ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca tttttttggt
     5881 gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc
     5941 gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggacctgc aggcatgcaa
     6001 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6061 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6121 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgagct
     6181 tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
     6241 ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg
     6301 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     6361 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     6421 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     6481 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     6541 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     6601 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     6661 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     6721 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     6781 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     6841 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     6901 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     6961 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     7021 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     7081 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     7141 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     7201 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     7261 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     7321 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     7381 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     7441 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     7501 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     7561 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     7621 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     7681 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     7741 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     7801 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     7861 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     7921 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     7981 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     8041 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     8101 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     8161 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     8221 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     8281 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     8341 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     8401 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     8461 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     8521 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     8581 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     8641 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     8701 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     8761 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     8821 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     8881 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     8941 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     9001 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     9061 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     9121 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     9181 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     9241 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     9301 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     9361 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     9421 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     9481 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     9541 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     9601 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     9661 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     9721 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     9781 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     9841 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     9901 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     9961 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    10021 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    10081 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    10141 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10201 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10261 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    10321 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11041 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
    11281 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    11341 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    11401 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    11461 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    11521 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    11581 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    11641 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    11701 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    11761 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    11821 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    11881 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    11941 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    12001 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    12061 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    12121 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    12181 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    12241 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    12301 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    12361 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    12421 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    12481 ggtcggggag ctgttggctg gctgg
//

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