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载体序列
LOCUS pMXs-IRES-Puro 5846 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5846)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5846
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 22..615
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 678..1035
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1094..1510
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1520..1894
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
misc_feature 2015..2566
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2578..3180
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
LTR 3351..3820
/note="long terminal repeat from Moloney murine leukemia
virus"
rep_origin complement(4121..4709)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4873..5733)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5734..5838)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
1921 cccagtgtgg tggtacggga attcctgcag gcctcgaggg ccggcgcgcc gcggccgcta
1981 cgtaaattcc gccccccccc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
2041 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttgcaatg
2101 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
2161 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
2221 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
2281 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
2341 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
2401 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
2461 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
2521 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
2581 gttaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
2641 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
2701 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
2761 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
2821 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
2881 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
2941 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
3001 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
3061 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
3121 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
3181 gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
3241 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
3301 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
3361 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
3421 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
3481 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
3541 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
3601 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
3661 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
3721 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
3781 tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat
3841 taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg
3901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
3961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
4321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4561 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
4621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
4681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
4741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
4801 tcttcaccta gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac
4861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
5041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
5101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
5161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
5221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
5281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
5401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
5521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5821 acaaataggg gttccgcgca catttc
//
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