pRetroX-Tight-Pur

价格:3500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pRetroX-Tight-Pur
质粒类型: 逆病毒载体;四环素调控载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Tight
载体大小: 6568 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pRetroX-Tight-Pur是四环素调控系统载体
产品目录号: 632104
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-Tight-Pur载体图谱



pRetroX-Tight-Pur 多克隆位点

pRetroX-Tight-Pur 载体特征

载体简介

pRetroX-Tight-Pur载体描述

pRetroX-Tight-Pur is an inducible, self-inactivating, retroviral expression vector designed to express a gene of interest under the control of PTight, a modified Tet-responsive promoter. PTight consists of seven direct repeats of a 36 bp regulatory sequence that contains the 19 bp tet operator sequence (tetO), and a modified minimal CMV promoter (1). This vector is supplied with our Retro-XTM Tet-On Advanced and Tet-Off Advanced Inducible Expression Systems (Cat. Nos. 632104 and 632105). These systems provide ready access to the inducible gene expression strategy of Gossen & Bujard, and incorporate major improvements described by Urlinger, et al. (2-6).
pRetroX-Tight-Pur is optimized to eliminate promoter interference through LTR self-inactivation. The hybrid 5’ LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This promoter drives high levels of viral genome transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (7-8) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3’ LTR enhancer region (U3). During reverse transcription of the retroviral RNA, a copy of the inactivated 3’ LTR U3 region replaces the corresponding region of the 5’ LTR, resulting in the inactivation of the 5’ LTR CMV enhancer sequence. This mechanism can reduce the phenomenon known as promoter interference (9-10) and allow more efficient expression. In this way, pRetroX-Tight-Pur supports high viral titers, yet eliminates potential downstream interference between the inducible PTight promoter and its adjacent viral LTR.

pRetroX-Tight-Pur contains all of the necessary viral RNA processing elements; these include the 5’ and 3’ LTRs, the packaging signal (Ψ+), and the tRNA primer binding site. For safety reasons, however, the vector lacks the structural genes (gag, pol, and env) necessary for retroviral particle formation and replication. pRetroX-Tight-Pur contains a puromycin resistance gene (Purr) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transfectants. In addition, the vector contains a ColE1 origin of replication and an E. coli Ampr gene for propagation and selection in bacteria.


pRetroX-Tight-Pur can be used as either a plasmid or retroviral expression vector. When used as a retroviral expression vector, it must be transfected into a packaging cell line, such as GP2-293; we recommend the Retro-X Universal Packaging System (Cat. No. 631530). Packaging cell lines allow you to produce infectious, replication-incompetent retroviral particles. These retroviral particles can infect a wide range of target cells and transmit your gene of interest, but they cannot replicate within these cells due to the absence of viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chance of producing replication-competent virus due to recombination events during cell proliferation.

Propagation in E. coli
 Suitable host strains: DH5α, DH10B and other general purpose strains.
 Selectable marker: plasmid confers resistance to ampicillin (100 μg/ml) in E. coli hosts.
 E. coli replication origin: ColE1
 Copy number: high

Notes:
The vector sequence was compiled from information in the sequence databases, published literature, and other sources, together with partial sequences obtained by Clontech. This vector has not been completely sequenced.
The viral supernatants produced by this retroviral vector could contain potentially hazardous recombinant virus. Due caution must be exercised in the production and handling of recombinant retrovirus. Appropriate NIH, regional, and institutional guidelines apply. 

载体序列

LOCUS       pRetroX-Tight-Pur	6567 bp 	DNA	SYN
DEFINITION  pRetroX-Tight-Pur
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="pRetroX-Tight-Pur"
                     /mol_type="other DNA"
     promoter        2..506
                     /label="CMV_immearly_promoter"
     misc_feature    6..688
                     /label="5_LTR"
     misc_feature    9..296
                     /label="CAG_enhancer"
     misc_feature    463..483
                     /label="CMV_fwd_primer"
     misc_feature    758..1567
                     /label="psi_plus_pack"
     misc_feature    1154..1561
                     /label="gag"
     misc_feature    1497..1519
                     /label="MSCV_primer"
     misc_feature    1497..1519
                     /label="pLXSN_5_primer"
     misc_feature    1535..1551
                     /label="pBABE_5_primer"
     CDS             1669..2196
                     /label="ORF frame 1"
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR
                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL
                     VRKFPPAPQLASCRT*"
     misc_feature    1888..1907
                     /label="pCEP_fwd_primer"
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR
                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL
                     VRKFPPAPQLASCRT*"
     misc_feature    1890..1914
                     /label="LNCX_primer"
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR
                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL
                     VRKFPPAPQLASCRT*"
     misc_feature    complement(2008..2031)
                     /label="MSCV_rev_primer"
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR
                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL
                     VRKFPPAPQLASCRT*"
     CDS             2253..3092
                     /label="ORF frame 3"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     misc_feature    2405..2424
                     /label="mPGK_F_primer"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     CDS             complement(2418..3143)
                     /label="ORF frame 2"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            2493..3092
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3801..3823)
                     /label="pGEX_3_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3959..3979)
                     /label="pBABE_3_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3965..4180)
                     /label="SV40_enhancer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     promoter        3977..4245
                     /label="SV40_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     rep_origin      4144..4221
                     /label="SV40_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    4206..4225
                     /label="SV40pro_F_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            complement(5345..6205)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     CDS             complement(5345..6205)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6247..6275)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6497..506
                     /label="CMV_immearly_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6561..688
                     /label="5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
   61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
  121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
  181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
  241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
  301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
  361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
  421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
  481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
  541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
  601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
  661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
  721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
  781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
  841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
  901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
  961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
 1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag
 1621 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
 1681 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
 1741 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
 1801 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
 1861 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
 1921 agaaggatcc gcggccgcgc cggctctaga tcgcgaacgc gtgaattcta ccgggtaggg
 1981 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
 2041 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
 2101 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
 2161 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
 2221 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
 2281 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
 2341 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
 2401 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
 2461 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
 2521 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
 2581 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
 2641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
 2701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
 2761 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
 2821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
 2881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
 2941 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
 3001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
 3061 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
 3121 ccgaaaggag cgcacgaccc catgcatcgg cgtctcgaga tatcagtggt ccaggctcta
 3181 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
 3241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
 3301 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
 3361 aagttcagat caaggtcagg aacagatgga acagggtcga ccctagagaa ccatcagatg
 3421 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
 3481 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
 3541 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
 3601 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
 3661 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
 3721 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt
 3781 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
 3841 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
 3901 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg
 3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
 4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
 4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
 4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
 4201 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
 4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat
 4321 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
 4381 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
 4441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
 4501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
 4561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
 4621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
 4681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
 4741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
 4801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
 4861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
 4921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
 4981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
 5041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
 5101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
 5161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
 5221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
 5281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
 5341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
 5401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
 5461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
 5521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
 5581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
 5641 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
 5701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
 5761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
 5821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
 5881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
 5941 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
 6001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
 6061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
 6121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
 6181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
 6241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
 6301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
 6361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt
 6421 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
 6481 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
 6541 cattagttca tagcccatat atggagt
//

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