pLVX-PTuner2-C

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-PTuner2-C
质粒类型: 慢病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8537 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DD tag (C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢毒载体pLVX-PTuner2-C载体表达N端DD标签融合蛋白;
DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域,带有DD标签的目的蛋白将很快被降解;
向培养基中加入Shield1配体后,情况将发生逆转,细胞中目的蛋白的量则迅速提高。
产品目录号: 631098
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-PTuner2-C载体图谱



pLVX-PTuner2-C 多克隆位点

pLVX-PTuner2-C 载体特征

载体简介

载体序列

LOCUS       pLVX-PTuner2-C   8537 bp    DNA   circular             8-SEP-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631098,631094
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4505..5096
                     /label=WPRE
     rep_origin      complement(6405..7078)
                     /label=pUC\origin
     CDS             complement(7223..8219)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5299..5935
                     /label=3'\LTR
     misc_feature    3363..3871
                     /label=PGK\Promoter
     misc_feature    3892..4491
                     /label=Puro(R)
     CDS             2868..3341
                     /label=DD-G
     misc_feature    2796..2867
                     /label=MCS
BASE COUNT     2134 a      2162 c      2246 g      1995 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccatc agtctgattg 
     2881 cggcgttagc ggtagatcac gttatcggca tggaaaacgc catgccgtgg aacctgcctg 
     2941 ccgatctcgc ctggtttaaa cgcaacacct taaataaacc cgtgattatg ggccgccata 
     3001 cctgggaatc aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc agcagtcaac 
     3061 cgagtacgga cgatcgcgta acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg 
     3121 gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt ctatgaacag ctcctgccaa 
     3181 aagcgcaaaa actgtatctg acgcatatcg acgcagaagt ggaaggcgac acccatttcc 
     3241 cggattacga gccggatgac tgggaatcgg tattcagcga attccacgat gctgatgcgc 
     3301 agaactctca cagctattgc tttgagattc tggagcggcg atgagcggcc gcgactctag 
     3361 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 
     3421 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 
     3481 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 
     3541 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 
     3601 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 
     3661 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 
     3721 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 
     3781 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 
     3841 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 
     3901 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 
     3961 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 
     4021 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 
     4081 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 
     4141 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 
     4201 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 
     4261 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 
     4321 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 
     4381 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 
     4441 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 
     4501 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     4561 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     4621 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     4681 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     4741 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     4801 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     4861 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     4921 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     4981 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     5041 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     5101 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 
     5161 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 
     5221 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     5281 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 
     5341 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 
     5401 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 
     5461 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 
     5521 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 
     5581 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 
     5641 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 
     5701 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 
     5761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
     5821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
     5881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 
     5941 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 
     6001 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 
     6061 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     6121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     6181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     6241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     6301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     6361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     6421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     6481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     6541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     6601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     6661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     6721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     6781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     6841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     6901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     6961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     7021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     7081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     7141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     7201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     7261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     7321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     7381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     7441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     7501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     7561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     7621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     7681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     7741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     7801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     7861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     7921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     7981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     8041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     8101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     8161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 
     8221 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     8281 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     8341 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 
     8401 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 
     8461 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 
     8521 actacaaact tagtagt 
//

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