pDONR201

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pDONR201
质粒类型: Gateway载体;克隆载体
高拷贝/低拷贝: 低拷贝
启动子: --
克隆方法: Gateway; BP反应
载体大小: 4470 bp
5' 测序引物及序列: pDONR-F: 5'-TCGCGTTAACGCTAGCATGGATCTC-3'
3' 测序引物及序列: pDONR-R: 5'-GTAACATCAGAGATTTTGAGACAC-3'
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素 / 氯霉素
筛选标记: --
备注:
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增,用来构建Gateway系统入门载体。

pDONR201尽管含有pUC质粒的起始复制点,但是细菌内仍然是低产量质粒。

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。 
 
产品目录号: 11798-014
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDONR201载体图谱



pDONR201多克隆位点

pDONR201载体特征

载体简介

载体序列

LOCUS       pDONR201	4470 bp    	DNA     circular    SYN
DEFINITION  pDONR201
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="pDONR201"
                     /mol_type="other DNA"
     terminator      complement(73..100)
                     /label="rrnB_T2_terminator"
     terminator      complement(232..275)
                     /label="rrnB_T1_terminator"
     misc_feature    complement(331..426)
                     /label="attL1"
     misc_feature    332..563
                     /label="attP"
     misc_feature    complement(430..530)
                     /label="attR2"
     misc_feature    430..530
                     /label="attR1"
     misc_feature    complement(959..1264)
                     /label="ccdB"
     gene            complement(1606..2265)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             complement(1606..2265)
                     /label="ORF frame 1"
     misc_feature    complement(2513..2744)
                     /label="attP"
     misc_feature    complement(2546..2653)
                     /label="attR1"
     misc_feature    2546..2646
                     /label="attR2"
     misc_feature    2670..2745
                     /label="attL2"
     gene            2868..3677
                     /label="KanR2"
                     /gene="KanR2"
     CDS             2868..3677
                     /label="ORF frame 3"
     rep_origin      3804..4423
                     /label="pBR322_origin"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCC AGGAAGAGTT
   61 TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA
  121 GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC
  181 GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA
  241 AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT
  301 CGCGTTAACG CTAGCATGGA TCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG
  361 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAG TTTGTACAAA
  421 AAAGCAGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
  481 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG AATCAACTAC
  541 TTAGATGGTA TTAGTGACCT GTAGTCGACC GACAGCCTTC CAAATGTTCT TCGGGTGATG
  601 CTGCCAACTT AGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA TCGTCGTATC
  661 CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT AAGAAAAAGA
  721 GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT ATACGCTAGT
  781 GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT ACTTTTCTCT
  841 TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC GTGATAAAGT
  901 TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT
  961 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT
 1021 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC
 1081 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA
 1141 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC
 1201 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT
 1261 GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG
 1321 ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT
 1381 TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT GCCTTGAGCA
 1441 ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA CACCTCTTTT
 1501 TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA
 1561 ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGATTACG CCCCGCCCTG
 1621 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA
 1681 GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA
 1741 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA
 1801 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT
 1861 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA
 1921 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG
 1981 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC
 2041 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA
 2101 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT
 2161 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA
 2221 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC
 2281 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG
 2341 AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG
 2401 CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC
 2461 AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG ACTACAGGTC
 2521 ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA
 2581 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT
 2641 TTCTCGTTCA GCTTTCTTGT ACAAAGTGGG CATTATAAGA AAGCATTGCT TATCAATTTG
 2701 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC
 2761 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT
 2821 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC
 2881 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
 2941 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC
 3001 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
 3061 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
 3121 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT
 3181 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
 3241 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
 3301 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
 3361 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC
 3421 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
 3481 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
 3541 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC
 3601 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC
 3661 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC
 3721 TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
 3781 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
 3841 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
 3901 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
 3961 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
 4021 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
 4081 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
 4141 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
 4201 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
 4261 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
 4321 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
 4381 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
 4441 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//

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