pCMV-LacZ

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pCMV-LacZ
质粒类型: 哺乳动物表达载体;β-gal报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: CMV
载体大小: 7.2 kb
5' 测序引物及序列: CMV Forward CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5a 或 HB101
宿主细胞(系): --
备注: pCMV-LacZ载体是β半乳糖甘酶表达载体,在哺乳动物细胞中高水平表达β半乳糖甘酶;
可用于建立标准浓度或者标准菌株,定量分析其它报告型载体的表达情况。
产品目录号: 631719
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-LacZ载体图谱



pCMV-LacZ 载体特征

载体简介


pCMV-LacZ is a mammalian reporter vector designed to expression β-galactosidase in mammalian cells from the human cytomegalovirus immediate early gene promoter (1). pCMV-LacZ contains an intron (splice donor/splice acceptor; 2) and polyadenylation signal from SV40, and the full-length E. coli β-galactosidase gene with eukaryotic translation initiation signals (3). pCMV-LacZ expresses high levels of β-galactosidase and can be used as a reference (control) plasmid when transfecting other reporter gene constructs and can be used to optimize transfection protocols by employing standard assays or stains to assay β-galactosidase activity. Alternatively, the β-galactosidase gene can be excised using the Not I sites at each end to allow other genes to be inserted into the pCMV-LacZ vector backbone for expression in mammalian cells or to insert the β-galactosidase fragment into another expression vector.

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