| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-LacZ，pCMVLacZ |
| 质粒类型: | 哺乳动物表达载体；β-gal报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 7.2 kb |
| 5' 测序引物及序列: | CMV Forward CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | DH5a 或 HB101 |
| 宿主细胞（系）: | -- |
| 备注: |
pCMV-LacZ载体是β半乳糖甘酶表达载体，在哺乳动物细胞中高水平表达β半乳糖甘酶； 可用于建立标准浓度或者标准菌株，定量分析其它报告型载体的表达情况。 |
| 产品目录号: | 631719 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-LacZ载体 pCMVLacZ质粒基本信息
pCMV-LacZ质粒图谱 pCMVLacZ载体图谱和pCMV-LacZ载体序列 pCMVLacZ质粒序列多克隆位点信息
pCMV-LacZ质粒 pCMVLacZ载体简介
pCMV-LacZ（pCMVLacZ） is a mammalian reporter vector designed to expression β-galactosidase in mammalian cells from the human cytomegalovirus immediate early gene promoter (1). pCMV-LacZ contains an intron (splice donor/splice acceptor; 2) and polyadenylation signal from SV40, and the full-length E. coli β-galactosidase gene with eukaryotic translation initiation signals (3). pCMV-LacZ expresses high levels of β-galactosidase and can be used as a reference (control) plasmid when transfecting other reporter gene constructs and can be used to optimize transfection protocols by employing standard assays or stains to assay β-galactosidase activity. Alternatively, the β-galactosidase gene can be excised using the Not I sites at each end to allow other genes to be inserted into the pCMV-LacZ vector backbone for expression in mammalian cells or to insert the β-galactosidase fragment into another expression vector.
pCMV-LacZ质粒序列 pCMVLacZ载体序列
LOCUS pCMV-LacZ 7164 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Plasmid for high-level expression of E. coli beta-galactosidase in
mammalian cells. Also known as pCMV-beta, pCMVbeta, or pCMV-beta.
ACCESSION U02451
VERSION .
KEYWORDS pCMV-LacZ
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7164)
AUTHORS MacGregor GR, Caskey CT.
TITLE Construction of plasmids that express E. coli beta-galactosidase in
mammalian cells.
JOURNAL Nucleic Acids Res. 1989;17:2365.
PUBMED 2495524
REFERENCE 2 (bases 1 to 7164)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Tuesday, September 8, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7164
/organism="Cloning vector pCMVbeta"
/mol_type="genomic DNA"
/db_xref="taxon:31798"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 873..965
/codon_start=1
/gene="ADH-71k "
/product="N-terminal fragment of Drosophila melanogaster
alcohol dehydrogenase"
/note="Drosophila Adh"
/translation="MSFTLTNKNVIFVAGLGGIGLDTSKELLKRD"
CDS 969..4016
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/note="lacZ"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAELQLSAGRYHYQLVWCQK"
polyA_signal 4303..4437
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4950..5538)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5709..6569)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6570..6674)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaattc ccggggatcg
841 aaagagcctg ctaaagcaaa aaagaagtca ccatgtcgtt tactttgacc aacaagaacg
901 tgattttcgt tgccggtctg ggaggcattg gtctggacac cagcaaggag ctgctcaagc
961 gcgatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
1021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
1081 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
1141 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
1201 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc
1261 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
1321 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
1381 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
1441 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
1501 tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
1561 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
1621 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
1681 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
1741 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
1801 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
1861 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
1921 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
1981 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
2041 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
2101 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
2161 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
2221 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
2281 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
2341 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
2401 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
2461 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
2521 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
2581 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
2641 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
2701 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
2761 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
2821 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
2881 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
2941 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
3001 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
3061 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
3121 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
3181 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
3241 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
3301 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
3361 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
3421 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
3481 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
3541 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
3601 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
3661 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
3721 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
3781 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
3841 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
3901 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
3961 aattacagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
4021 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa
4081 aaaacacaaa cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag
4141 cctacttccc gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata
4201 cgggtattat ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc
4261 tgctttctga caaactcggc ctcgactcta ggcggccgcg gggatccaga catgataaga
4321 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
4381 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
4441 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg
4501 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
4561 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
4621 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
4681 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
4741 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
4801 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
4861 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
4921 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
4981 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
5041 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
5101 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
5161 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
5221 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
5281 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
5341 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
5401 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
5461 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
5521 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
5581 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
5641 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
5701 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
5761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
5821 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
5881 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
5941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
6001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
6061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
6121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
6181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
6241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
6301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
6361 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
6421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
6481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
6541 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
6601 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
6661 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
6721 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
6781 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
6841 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
6901 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
6961 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
7021 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
7081 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
7141 cgacgttgta aaacgacggc cagt
//
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pCMV-LacZ质粒 pCMVLacZ载体应用举例
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