pAc5-V5-HisC

价格:1600元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pAc5-V5-HisC 、pAc5/V5-His C
质粒类型: 昆虫表达载体;果蝇细胞表达载体
克隆方法: 多克隆位点,限制性内切酶
启动子: P-AC5 (Actin 5C)
载体大小: 5.4kb
5' 测序引物及序列: AC5 Forward:5'd[ACACAAAGCCGCTCCATCAG]3'
3' 测序引物及序列: --
载体标签: V5 Epitope Tag , His Tag (C-末端)
载体抗性: 氨苄青霉素
真核筛选标记:
克隆菌株: TOP10;DH5-T1R
宿主细胞(系): 果蝇细胞系S2
备注: pAc5-V5-His 载体可以与筛选载体pCoHygro或者pCoBlast共转染,建立稳表达细胞系。
产品目录号: V4110-20
瞬表达/稳表达: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pAc5-V5-HisC载体图谱



pAc5-V5-HisC 多克隆位点

载体简介

载体序列

CTTCAAGGAATTAATTCTATATTCTAAAAACACAAATGATACTTCTAAAAAAAATCATGAATGGCATCAACT
CTGAATCAAATCTTTGCAGATGCACCTACTTCTCATTTCCACTGTCACATCATTTTTCCAGATCTCGCTGCC
TGTTATGTGGCCCACAAACCAAGACACGTTTTATGGCCATTAAAGCTGGCTGATCGTCGCCAAACACCAAAT
ACATATCAATATGTACATTCGAGAAAGAAGCGATCAAAGAAGCGTCTTCGGGCGAGTAGGAGAATGCGGAGG
AGAAGGAGAACGAGCTGATCTAGTATCTCTCCACAATCCAATGCCAACTGACCAACTGGCCATATTCGGAGC
AATTTGAAGCCAATTTCCATCGCCTGGCGATCGCTCCATTCTTGGCTATATGTTTTTCACCGTTCCCGGGGC
CATTTTCAAAGACTCGTCGGTAAGATAAGATTGTGTCACTCGCTGTCTCTCTTCATTTGTCGAAGAATGCTG
AGGAATTTCGCGATGACGTCGGCGAGTATTTTGAAGAATGAGAATAATTTGTATTTATACGAAAATCAGTTA
GTGGAATTTTCTACAAAAACATGTTATCTATAGATAATTTTGTTGCAAAATATGTTGACTATGACAAAGATT
GTATGTATATACCTTTAATGTATTCTCATTTTCTTATGTATTTATAATGGCAATGATGATACTGATGATATT
TTAAGATGATGCCAGACCACAGGCTGATTTCTGCGTCTTTTGCCGAACGCAGTGCATGTGCGGTTGTTGTTT
TTTGGAATAGTTTCAATTTTCGGACTGTCCGCTTTGATTTCAGTTTCTTGGCTTATTCAAAAAGCAAAGTAA
AGCCAAAAAAGCGAGATGGCAATACCAAATGCGGCAAAACGGTAGTGGAAGGAAAGGGGTGCGGGGCAGCGG
AAGGAAGGGTGGGGCGGGGCGTGGCGGGGTCTGTGGCTGGGCGCGACGTCACCGACGTTGGAGCCACTCCTT
TGACCATGTGTGCGTGTGTGTATTATTCGTGTCTCGCCACTCGCCGGTTGTTTTTTTCTTTTTATCTCGCTC
TCTCTAGCGCCATCTCGTACGCATGCTCAACGCACCGCATGTTGCCGTGTCCTTTATGCGTCATTTTGGCTC
GAAATAGGCAATTATTTAAACAAAGATTAGTCAACGAAAACGCTAAAATAAATAAGTCTACAATATGGTTAC
TTATTGCCATGTGTGTGCAGCCAACGATAGCAACAAAAGCAACAACACAGTGGCTTTCCCTCTTTCACTTTT
TGTTTGCAAGCGCGTGCGAGCAAGACGGCACGACCGGCAAACGCAATTACGCTGACAAAGAGCAGACGAAGT
TTTGGCCGAAAAACATCAAGGCGCCTGATACGAATGCATTTGCAATAACAATTGCGATATTTAATATTGTTT
ATGAAGCTGTTTGACTTCAAAACACACAAAAAAAAAAATAAAACAAATTATTTGAAAGAGAATTAGGAATCG
GACAGCTTATCGTTACGGGCTAACAGCACACCGAGACGAAATAGCTTACCTGACGTCACAGCCTCTGGAAGA
ACTGCCGCCAAGCAGAGAGAGAGAGAAAAAGAGGGAGAGCAGCTTAGACCGCATGTGCTTGTGTGTGAGGCG
TCTCTCTCTTCGTCTCCTGTTTGCGCAAACGCATAGACTGCACTGAGAAAATCGATTACCTATTTTTTATGA
ATGAATATTTGCACTATTACTATTCAAAACTATTAAGATAGCAATCACATTCAATAGCCAAATACTATACCA
CCTGAGCGATGCAACGAAATGATCAATTTGAGCAAAAATGCTGCATATTTAGGACGGCATCATTATAGAAAT
GCTTCTTGCTGTGTACTTTTCTCTCGTCTGGCAGCTGTTTCGCCGTTATTGTTAAAACCGGCTTAAGTTAGG
TGTGTTTTCTACGACTAGTGATGCCCCTACTAGAAGATGTGTGTTGCACAAATGTCCCTGAATAACCAATTT
GAAGTGCAGATAGCAGTAAACGTAAGCTAATATGAATATTATTTAACTGTAATGTTTTAATATCGCTGGACA
TTACTAATAAACCCACTATAAACACATGTACATATGTATGTTTTGGCATACAATGAGTAGTTGGGGAAAAAA
TGTGTAAAAGCACCGTGACCATCACAGCATAAAGATAACCAGCTGAAGTATCGAATATGAGTAACCCCCAAA
TTGAATCACATGCCGCAACTGATAGGACCCATGGAAGTACACTCTTCATGGCGATATACAAGACACACACAA
GCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAAATGAAAACCCAATCGGCGAACAATTCATACCCATAT
ATGGTAAAAGTTTTGAACGCGACTTGAGAGCGGAGAGCATTGCGGCTGATAAGGTTTTAGCGCTAAGCGGGC
TTTATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTGAGTTCTTGTGCTGTGTGGATACTCCT
CCCGACACAAAGCCGCTCCATCAGCCAGCAGTCGTCTAATCCAGAGACCCCGGATCCGATGGGGTACCTACT
AGTCCAGTGTGGTGGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGGTCACCCATTCGAAGGTAA
GCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTA
AACCCGCTGATCAGCCTCGACTGTGCCTTCTAAGGCCTGAGCTCGCTGATCAGCCTCGATCGAGGATCCAGA
CATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGA
AATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCAT
TCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGG
TATGGCTGATTATGATCAGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGC
GGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAG
CGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTAT
TCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGG
CCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGG
CCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGC
AGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACC
AATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCG
TCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCAC
GCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAA
CTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTT
TGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCT
CCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTC
CTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTC
TTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGT
GTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAA
AAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTT
CGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAA
AAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCC
TTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGA
AAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTA
TCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGT

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载体应用举例

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