Cas9 MDC123

LOCUS       Cas9 MDC123             13658 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    Cas9 MDC123
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 13658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    82..106
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     294..316
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     308..325
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     309..325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        751..1095
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1039..1059
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1156..5259
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             5172..5180
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5266..5286
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5625..5641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5638..5660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5649..5665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5673..5703)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5718..5739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5930..6607
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             6652..7203
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    7210..7384
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7462..7486
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7911..8705
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8792..9380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9281..9300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9534..9551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9566..9706
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9632..9651)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9792..9814
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10050..10244
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10310..11377)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(11811..12440)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
       61 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
      121 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
      181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      301 acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtca acatggtgga
      361 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      421 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      541 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      601 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      661 agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca aaaatatcaa
      721 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
      781 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
      841 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
      901 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
      961 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     1021 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     1081 tcatttggag aggacctcga ctctagagga tccccgggta ccgggccccc cctcgaggcg
     1141 cgccaccagg tcgacatgga taagaagtac tccatcggac tggatatcgg aactaactcc
     1201 gtgggatggg ctgtgatcac tgatgagtac aaggtgccat ccaagaagtt caaggtgctg
     1261 ggaaacactg atagacactc catcaagaag aacctgatcg gagctctgct gttcgattcc
     1321 ggagagactg ctgaggctac tagactgaag agaactgcta gaagaagata cactagaaga
     1381 aagaacagaa tctgctacct gcaagagatc ttctccaacg agatggctaa ggtggatgat
     1441 tccttcttcc acagactgga ggagtccttc ctggtggagg aggataagaa gcacgagaga
     1501 cacccaatct tcggaaacat cgtggatgag gtggcttacc acgagaagta cccaactatc
     1561 taccacctga gaaagaagct ggtggattcc actgataagg ctgatctgag actgatctac
     1621 ctggctctgg ctcacatgat caagttcaga ggacacttcc tgatcgaggg agatctgaac
     1681 ccagataact ccgatgtgga taagctgttc atccaactgg tgcaaactta caaccaactg
     1741 ttcgaggaga acccaatcaa cgcttccgga gtggatgcta aggctatcct gtccgctaga
     1801 ctgtccaagt ccagaagact ggagaacctg atcgctcaac tgccaggaga gaagaagaac
     1861 ggactgttcg gaaacctgat cgctctgtcc ctgggactga ctccaaactt caagtccaac
     1921 ttcgatctgg ctgaggatgc taagctgcaa ctgtccaagg atacttacga tgatgatctg
     1981 gataacctgc tggctcaaat cggagatcaa tacgctgatc tgttcctggc tgctaagaac
     2041 ctgtccgatg ctatcctgct gtccgatatc ctgagagtga acactgagat cactaaggct
     2101 ccactgtccg cttccatgat caagagatac gatgagcacc accaagatct gactctgctg
     2161 aaggctctgg tgagacaaca actgccagag aagtacaagg agatcttctt cgatcaatcc
     2221 aagaacggat acgctggata catcgatgga ggagcttccc aagaggagtt ctacaagttc
     2281 atcaagccaa tcctggagaa gatggatgga actgaggagc tgctggtgaa gctgaacaga
     2341 gaggatctgc tgagaaagca aagaactttc gataacggat ccatcccaca ccaaatccac
     2401 ctgggagagc tgcacgctat cctgagaaga caagaggatt tctacccatt cctgaaggat
     2461 aacagagaga agatcgagaa gatcctgact ttcagaatcc catactacgt gggaccactg
     2521 gctagaggaa actccagatt cgcttggatg actagaaagt ccgaggagac tatcactcca
     2581 tggaacttcg aggaggtggt ggataaggga gcttccgctc aatccttcat cgagagaatg
     2641 actaacttcg ataagaacct gccaaacgag aaggtgctgc caaagcactc cctgctgtac
     2701 gagtacttca ctgtgtacaa cgagctgact aaggtgaagt acgtgactga gggaatgaga
     2761 aagccagctt tcctgtccgg agagcaaaag aaggctatcg tggatctgct gttcaagact
     2821 aacagaaagg tgactgtgaa gcaactgaag gaggattact tcaagaagat cgagtgcttc
     2881 gattccgtgg agatctccgg agtggaggat agattcaacg cttccctggg aacttaccac
     2941 gatctgctga agatcatcaa ggataaggat ttcctggata acgaggagaa cgaggatatc
     3001 ctggaggata tcgtgctgac tctgactctg ttcgaggata gagagatgat cgaggagaga
     3061 ctgaagactt acgctcacct gttcgatgat aaggtgatga agcaactgaa gagaagaaga
     3121 tacactggat ggggaagact gtccagaaag ctgatcaacg gaatcagaga taagcaatcc
     3181 ggaaagacta tcctggattt cctgaagtcc gatggattcg ctaacagaaa cttcatgcaa
     3241 ctgatccacg atgattccct gactttcaag gaggatatcc aaaaggctca agtgtccgga
     3301 caaggagatt ccctgcacga gcacatcgct aacctggctg gatccccagc tatcaagaag
     3361 ggaatcctgc aaactgtgaa ggtggtggat gagctggtga aggtgatggg aagacacaag
     3421 ccagagaaca tcgtgatcga gatggctaga gagaaccaaa ctactcaaaa gggacaaaag
     3481 aactccagag agagaatgaa gagaatcgag gagggaatca aggagctggg atcccaaatc
     3541 ctgaaggagc acccagtgga gaacactcaa ctgcaaaacg agaagctgta cctgtactac
     3601 ctgcaaaacg gaagagatat gtacgtggat caagagctgg atatcaacag actgtccgat
     3661 tacgatgtgg atcacatcgt gccacaatcc ttcctgaagg atgattccat cgataacaag
     3721 gtgctgacta gatccgataa gaacagagga aagtccgata acgtgccatc cgaggaggtg
     3781 gtgaagaaga tgaagaacta ctggagacaa ctgctgaacg ctaagctgat cactcaaaga
     3841 aagttcgata acctgactaa ggctgagaga ggaggactgt ccgagctgga taaggctgga
     3901 ttcatcaaga gacaactggt ggagactaga caaatcacta agcacgtggc tcaaatcctg
     3961 gattccagaa tgaacactaa gtacgatgag aacgataagc tgatcagaga ggtgaaggtg
     4021 atcactctga agtccaagct ggtgtccgat ttcagaaagg atttccaatt ctacaaggtg
     4081 agagagatca acaactacca ccacgctcac gatgcttacc tgaacgctgt ggtgggaact
     4141 gctctgatca agaagtaccc aaagctggag tccgagttcg tgtacggaga ttacaaggtg
     4201 tacgatgtga gaaagatgat cgctaagtcc gagcaagaga tcggaaaggc tactgctaag
     4261 tacttcttct actccaacat catgaacttc ttcaagactg agatcactct ggctaacgga
     4321 gagatcagaa agagaccact gatcgagact aacggagaga ctggagagat cgtgtgggat
     4381 aagggaagag atttcgctac tgtgagaaag gtgctgtcca tgccacaagt gaacatcgtg
     4441 aagaagactg aggtgcaaac tggaggattc tccaaggagt ccatcctgcc aaagagaaac
     4501 tccgataagc tgatcgctag aaagaaggat tgggatccaa agaagtacgg aggattcgat
     4561 tccccaactg tggcttactc cgtgctggtg gtggctaagg tggagaaggg aaagtccaag
     4621 aagctgaagt ccgtgaagga gctgctggga atcactatca tggagagatc ctccttcgag
     4681 aagaacccaa tcgatttcct ggaggctaag ggatacaagg aggtgaagaa ggatctgatc
     4741 atcaagctgc caaagtactc cctgttcgag ctggagaacg gaagaaagag aatgctggct
     4801 tccgctggag agctgcaaaa gggaaacgag ctggctctgc catccaagta cgtgaacttc
     4861 ctgtacctgg cttcccacta cgagaagctg aagggatccc cagaggataa cgagcaaaag
     4921 caactgttcg tggagcaaca caagcactac ctggatgaga tcatcgagca aatctccgag
     4981 ttctccaaga gagtgatcct ggctgatgct aacctggata aggtgctgtc cgcttacaac
     5041 aagcacagag ataagccaat cagagagcaa gctgagaaca tcatccacct gttcactctg
     5101 actaacctgg gagctccagc tgctttcaag tacttcgata ctactatcga tagaaagaga
     5161 tacacttcca ctaaggaggt gctggatgct actctgatcc accaatccat cactggactg
     5221 tacgagacta gaatcgatct gtcccaactg ggaggagatc tgcaaccaaa gaagaagaga
     5281 aaggtgggag gatgatctag accagattaa tataactagt tctagagcgg ccgcccaccg
     5341 cggtggagct cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg
     5401 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     5461 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     5521 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     5581 ttatcgcgcg cggtgtcatc tatgttactg aattcgtaat catggtcata gctgtttcct
     5641 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5701 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5761 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5821 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
     5881 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     5941 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     6001 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     6061 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     6121 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     6181 ataacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     6241 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     6301 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     6361 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     6421 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     6481 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     6541 cccactatcc ttcgcaagac cttcctctat ataaggaagt tcatttcatt tggagaggac
     6601 acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagtctac catgagccca
     6661 gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg acatgccggc ggtctgcacc
     6721 atcgtcaacc actacatcga gacaagcacg gtcaacttcc gtaccgagcc gcaggaaccg
     6781 caggagtgga cggacgacct cgtccgtctg cgggagcgct atccctggct cgtcgccgag
     6841 gtggacggcg aggtcgccgg catcgcctac gcgggcccct ggaaggcacg caacgcctac
     6901 gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac gggactgggc
     6961 tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc
     7021 gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc
     7081 ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc
     7141 tggcagctgg acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatt
     7201 tgactcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     7261 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     7321 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     7381 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     7441 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     7501 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     7561 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     7621 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     7681 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     7741 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     7801 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     7861 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     7921 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     7981 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     8041 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     8101 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     8161 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     8221 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     8281 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     8341 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     8401 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     8461 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     8521 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     8581 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     8641 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     8701 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9481 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9541 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     9601 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9661 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     9721 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9781 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9841 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     9901 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     9961 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
    10021 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
    10081 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
    10141 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
    10201 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
    10261 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
    10321 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
    10381 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
    10441 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
    10501 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
    10561 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
    10621 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
    10681 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
    10741 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
    10801 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
    10861 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
    10921 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
    10981 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
    11041 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
    11101 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
    11161 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
    11221 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
    11281 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
    11341 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg
    11401 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    11461 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    11521 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    11581 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    11641 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    11701 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    11761 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    11821 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    11881 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11941 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    12001 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    12061 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    12121 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    12181 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    12241 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    12301 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    12361 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    12421 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    12481 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    12541 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    12601 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    12661 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    12721 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    12781 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    12841 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12901 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12961 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    13021 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    13081 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    13141 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    13201 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    13261 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    13321 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    13381 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    13441 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    13501 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    13561 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    13621 agtactttga tcccgagggg aaccctgtgg ttggcatg
//