pEGM-11ZF(+)

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联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pEGM-11ZF(+)
质粒类型: 大肠杆菌克隆载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3223bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEGM-11ZF(+)载体图谱



pEGM-11ZF(+)多克隆位点

pEGM-11ZF(+)载体特征

载体简介

The pGEM?-11Zf(?) Vector(a) can be used as a standard cloning vector, as a template
for in vitro transcription and for the production of ssDNA. The plasmid contains T7 and
SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide
coding region of β-galactosidase (1). Insertional inactivation of the α-peptide allows
recombinant clones to be directly identified by color screening on indicator plates when
using appropriate E. coli strains (e.g., JM109, DH5α?, XL1-Blue). The multiple cloning
region contains unique restriction sites for Sfi I, Sac I, EcoR I, Sal I, Xho I, BamH I,
Apa I, Xba I, Not I, Sph I, Nsi I and Hind III.
For induction of ssDNA, bacterial cells with the F′ episome (e.g., JM109, XL1-Blue,
DH5α?F′) containing pGEM?-11Zf(?) recombinants are infected with an appropriate
helper phage. The plasmid then enters the f1 replication mode, and the resulting
ssDNA is exported from the cell as an encapsidated virus-like particle. The sequence of
the ssDNA rescued upon infection with helper phage is the same strand as that shown
in Figure 1. The exported ssDNA can be used for in vitro mutagenesis or can be
sequenced using Promega's SP6 Promoter Primer or pUC/M13 Reverse Primer.
The sequences of Promega vectors are available online at www.promega.com/vectors/
and are also available from the GenBank? database.

载体序列

LOCUS       pGEM-11Zf-	3223 bp 	DNA	SYN
DEFINITION  pGEM-11Zf-
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="pGEM-11Zf-"
                     /mol_type="other DNA"
     misc_feature    complement(93..110)
                     /label="Sp6_primer"
     promoter        complement(124..142)
                     /label="M13_reverse_primer"
     misc_feature    complement(141..163)
                     /label="M13_pUC_rev_primer"
     promoter        complement(177..206)
                     /label="lac_promoter"
     rep_origin      complement(515..1134)
                     /label="pBR322_origin"
     gene            complement(1289..2149)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1289..2149)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2191..2219)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(2378..2400)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2605..2911
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(3055..3203)
                     /label="lacZ_a"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3169..3191
                     /label="M13_pUC_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        3184..3200
                     /label="M13_forward20_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        3207..2
                     /label="T7_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgagggat ccgggccctc
   61 tagatgcggc cgcatgcata agcttgagta ttctatagtg tcacctaaat agcttggcgt
  121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
  181 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
  241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
  301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
  361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
  421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
  481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttcgataggc
  541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
  601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
  661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
  721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
  781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
  841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
  901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
  961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
 1021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
 1081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
 1141 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
 1201 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
 1261 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
 1321 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
 1381 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
 1441 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
 1501 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
 1561 gtagttcgcc agttaatagt ttgcgcaacg ttgttggcat tgctacaggc atcgtggtgt
 1621 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
 1681 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
 1741 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
 1801 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
 1861 gagaataccg cgcccggcga ccgagttgct cttgcccggc gtcaatacgg gataatagtg
 1921 tatgacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
 1981 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
 2041 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
 2101 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
 2161 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
 2221 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
 2281 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
 2341 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
 2401 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
 2461 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
 2521 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
 2581 tcaggcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
 2641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
 2701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
 2761 cgatttagag ctttacggca cctcgaccgc aaaaaacttg atttgggtga tggttcacgt
 2821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
 2881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
 2941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
 3001 atatttaacg cgaattttaa caaaatatta acgtttacaa tttccattcg ccattcaggc
 3061 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
 3121 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
 3181 gttgtaaaac gacggccagt gaattgtaat acgactcact ata
//

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