pAAV-minCMV-mCherry

价格:1100元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pAAV-minCMV-mCherry
质粒类型: 腺病毒载体;荧光蛋白报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Minimal CMV
载体大小: 4887 bp
5' 测序引物及序列: ccgagggcttcaagtgggagcgc
3' 测序引物及序列: cagcttcaccttgtagatgaactcgc
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 红色荧光蛋白mCherry
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 常规哺乳动物细胞系
备注: --
产品目录号: #27970
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-minCMV-mCherry载体图谱



载体简介

载体序列

LOCUS       pAAV-minCMV-mCherry	4887 bp 	DNA	SYN
DEFINITION  pAAV-minCMV-mCherry
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-minCMV-mCherry|
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="pAAV-minCMV-mCherry"
                     /mol_type="other DNA"
     promoter        163..181
                     /label="T7_promoter"
     misc_feature    233..252
                     /label="pCEP_fwd_primer"
     misc_feature    235..259
                     /label="LNCX_primer"
     CDS             279..989
                     /label="ORF frame 3"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     gene            282..986
                     /label="mCherry"
                     /gene="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    459..512
                     /label="Q66M (mCherry)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    1016..1603
                     /label="WPRE"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     CDS             1104..1715
                     /label="ORF frame 3"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     SSSFPWLLAYVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
     CDS             1117..1650
                     /label="ORF frame 1"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     terminator      1632..2110
                     /label="hGH_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     rep_origin      2382..2688
                     /label="f1_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     misc_feature    2956..2978
                     /label="pGEX_3_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     promoter        3137..3165
                     /label="AmpR_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     gene            3207..4067
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     CDS             3207..4067
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4222..4841
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
   61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
  121 actccatcac taggggttcc tgcggccgca cgcgtATCTA GATAATACGA CTCACTATAG
  181 GGGGATCCAC GTATGTCGAG GTAGGCGTGT ACGGTGGGAG GCCTATATAA GCAGAGCTCG
  241 TTTAGTGAAC CGTCAGATCG CCTGGAGGTA CCGCCACCAT GGTGAGCAAG GGCGAGGAGG
  301 ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA
  361 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA
  421 CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC
  481 CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT
  541 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
  601 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA
  661 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT
  721 GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC
  781 AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG
  841 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
  901 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  961 CCGGCGGCAT GGACGAGCTG TACAAGTAAG AATTCgatat caagcttatc gataatcaac
 1021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
 1081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
 1141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
 1201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
 1261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
 1321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
 1381 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg
 1441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
 1501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
 1561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
 1621 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
 1681 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
 1741 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
 1801 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
 1861 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
 1921 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
 1981 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
 2041 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
 2101 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta
 2161 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
 2221 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
 2281 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
 2341 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
 2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
 2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
 2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
 2581 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
 2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
 2701 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
 2761 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
 2821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
 2881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
 2941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
 3001 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
 3061 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
 3121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
 3181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
 3241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
 3301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
 3361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
 3421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
 3481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
 3541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
 3601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
 3661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
 3721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
 3781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
 3841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
 3901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
 3961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
 4021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
 4081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
 4141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
 4201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
 4261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
 4321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
 4381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
 4441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
 4501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
 4561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
 4621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
 4681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
 4741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
 4801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
 4861 ccttttgctg gccttttgct cacatgt
//

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