| 质粒名称: | pSLIK-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 66844 |
| 存储实验室: | Christian Metallo |
|
载体骨架基本信息 |
|
| 载体名称: | pSLIK-hygro |
| 载体出品公司: | Ian Frasier, Addgene Plasmid #25737 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 13918 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | Aequorea victoria |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | huB-Cpro-R |
|
文献引用方法 |
|
| 材料和方法部分: | pSLIK-GFP was a gift from Christian Metallo (Addgene plasmid # 66844 ; http://n2t.net/addgene:66844 ; RRID:Addgene_66844) |
| 参考文献部分: | Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes NI, Feist AM, Vander Heiden MG, Metallo CM. Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29. 10.1016/j.molcel.2014.05.008 PubMed 24882210 |
买家导航
pSLIK-GFP载体 pSLIKGFP质粒基本信息
pSLIK-GFP质粒图谱 pSLIKGFP载体图谱和pSLIK-GFP载体序列 pSLIKGFP质粒序列多克隆位点信息
pSLIK-GFP质粒 pSLIKGFP载体简介
pSLIK-GFP质粒序列 pSLIKGFP载体序列
LOCUS pSLIK-GFP 13529 bp ds-DNA circular SYN 21-DEC-2018
DEFINITION Tet-inducible expression of GFP in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pSLIK-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13529)
AUTHORS Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes
NI, Feist AM, Vander Heiden MG, Metallo CM
TITLE Tracing compartmentalized NADPH metabolism in the cytosol and
mitochondria of mammalian cells.
JOURNAL Mol Cell. 2014 Jul 17;55(2):253-63. doi:
10.1016/j.molcel.2014.05.008. Epub 2014 May 29.
PUBMED 24882210
REFERENCE 2 (bases 1 to 13529)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 21, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13529
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2203..2320
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2377..2401
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind 2440..2710
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2743..2781
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2778..2802
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2888..3607
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2933..2954)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3194..3213)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3541..3562
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(3660..3684)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter 3734..4945
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 4782..4801
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 5070..5816
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 5974..6436
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6030..6047)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6257..6276
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 6495..7514
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
misc_feature 7980..8568
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8033..8053)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8451..8462)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8571..8587)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8572..8588)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 9097..9277
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9303..9320)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9309..9533
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9579..10007
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9666..9685)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9876..9897
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10016..10036)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10021..10350
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10201..10336
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10263..10282
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10398..10445
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10464..10838
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10968..11089
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11005..11024)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11059..11078
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11138..11154)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11138..11154)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11151..11173)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11162..11178
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11186..11216)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11231..11252
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11369..11386)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11540..12128)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11620..11639)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12299..13159)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12922..12941
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13160..13264)
/gene="bla"
/label=AmpR promoter
primer_bind complement(13339..13358)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
2401 tttaaaggaa ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa
2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
2521 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
2581 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
2641 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
2701 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg
2761 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
2821 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaacta
2881 gtccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
2941 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
3001 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
3061 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
3121 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
3181 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
3241 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
3301 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
3361 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
3421 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
3481 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
3541 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
3601 caagtaaact agtcgactag ggataacagg gtaattgttt gaatgaggct tcagtctaga
3661 cccagctttc ttgtacaaag tggttgataa catctcgagc cataaagatg gttaattaac
3721 ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc
3781 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg
3841 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag
3901 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc
3961 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc
4021 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag
4081 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc
4141 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt
4201 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg
4261 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg
4321 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag
4381 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc
4441 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg
4501 cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg
4561 ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct
4621 gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta
4681 ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca
4741 gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac
4801 tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta
4861 atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg
4921 gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatct ccaccccgta
4981 ccggtcctgc aggatttaaa tggcgcgccg ctagcgctac cggactcaga tctcgagctc
5041 aagcttcgaa ttctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa
5101 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
5161 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
5221 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
5281 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
5341 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
5401 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
5461 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
5521 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
5581 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
5641 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
5701 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
5761 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
5821 gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc cctncccccc
5881 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
5941 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
6001 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
6061 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
6121 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
6181 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
6241 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
6301 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
6361 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
6421 aaaaacacga tgataagctt accggtacgc gtgatagctt gccacaaccc gtaccaaaga
6481 tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
6541 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
6601 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
6661 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
6721 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
6781 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
6841 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
6901 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
6961 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
7021 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
7081 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
7141 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
7201 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
7261 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
7321 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
7381 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
7441 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
7501 gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc
7561 agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac
7621 agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt
7681 gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc
7741 gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca
7801 gatctctaga tctgtacaag taaagcggcc gcgactctag atcataatca gccataccac
7861 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
7921 taaaatgaat gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgata
7981 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
8041 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
8101 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
8161 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
8221 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
8281 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
8341 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
8401 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
8461 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
8521 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
8581 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
8641 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
8701 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
8761 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
8821 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
8881 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
8941 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
9001 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
9061 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
9121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
9181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
9241 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
9301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
9361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
9421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
9481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
9541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
9601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
9661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
9721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
9781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
9841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
9901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
9961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
10021 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
10081 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
10141 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
10201 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
10261 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
10321 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
10381 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
10441 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
10501 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
10561 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
10621 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
10681 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
10741 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
10801 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
10861 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
10921 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
10981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
11041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
11101 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
11161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
11221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
11281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
11341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
11401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
11461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
11521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
11581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
11641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
11701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
11761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
11821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
11881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
11941 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
12001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
12061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
12121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
12181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
12241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
12301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
12361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
12421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
12481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
12541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
12601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
12661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
12721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
12781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
12841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
12901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
12961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
13021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
13081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
13141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
13201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
13261 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
13321 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
13381 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
13441 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
13501 tcgcgatgta cgggccagat atacgcgtt
//
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pSLIK-GFP质粒 pSLIKGFP载体应用举例
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