| 质粒名称: | pAC-85b |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 53282 |
| 存储实验室: | Francis X Cunningham Jr |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC184 |
| 载体出品公司: | New England BioLabs |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4245 bp |
| 完整质粒大小: | 9424 bp |
| 载体修饰: | 质粒pAC-BETA用EcoRI酶切，BamHI部分酶切，凝胶纯化7.1kb片段。质粒pACYC184经EcoRI和BamHI酶切，凝胶纯化2.4kb片段。将两个片段连接在一起得到质粒pAC-85b。 |
| 载体类型: | 低拷贝数细菌克隆载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | crtE, crtY, crtI |
| 物种来源: | Erwinia herbicola Eho10 |
| 插入大小: | 7048 bp |
| 基因突变: | -- |
| GenBank编号 | M87280.1 |
| 启动子 | endogenous promoters |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAC-85b was a gift from Francis X Cunningham Jr (Addgene plasmid # 53282 ; http://n2t.net/addgene:53282 ; RRID:Addgene_53282) |
| 参考文献部分: | A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Cunningham FX Jr, Gantt E. Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. 10.1007/s11120-007-9210-0 PubMed 17634749 |
买家导航
pAC85b载体基本信息
pAC85b载体图谱质粒图谱和多克隆位点信息
pAC85b载体简介
pAC85b载体序列
LOCUS pAC-85b 9424 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea
agglomerans) Eho10, and will produce beta-carotene in E. coli when
complemented with a gene encoding phytoene synthase.
ACCESSION .
VERSION .
KEYWORDS pAC-85b
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9424)
AUTHORS Cunningham FX Jr, Gantt E
TITLE A portfolio of plasmids for identification and analysis of
carotenoid pathway enzymes: Adonis aestivalis as a case study.
JOURNAL Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
PUBMED 17634749
REFERENCE 2 (bases 1 to 9424)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9424
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 133..152
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(219..321)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(713..730)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(847..1392)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(927..946)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 1504..1532
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(7111..7130)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(8983..218)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
61 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
121 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
181 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
241 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
301 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
361 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
421 tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg
481 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
541 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg
601 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
661 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
721 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
781 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
841 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
901 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
1861 cgtcctgtgg atctaaaggc acagcgtctc atgcttcgca caatgtaaaa ctgcttcaga
1921 acctggcgag agctatccgc gcggtctacg gttaactgat actaaaagac aattcagcgg
1981 gtaaccttgc aatggtgagt ggcagtaaag cgggcgtttc gcctcatcgc gaaatagaag
2041 taatgagaca atccattgac gatcacctgg ctggcctgtt acctgaaacc gacagccagg
2101 atatcgtcag ccttgcgatg cgtgaaggcg tcatggcacc cggtaaacgg atccgtccgc
2161 tgctgatgct gctggccgcc cgcgacctcc gctaccaggg cagtatgcct acgctgctcg
2221 atctcgcctg cgccgttgaa ctgacccata ccgcgtcgct gatgctcgac gacatgccct
2281 gcatggacaa cgccgagctg cgccgcggtc agcccactac ccacaaaaaa tttggtgaga
2341 gcgtggcgat ccttgcctcc gttgggctgc tctctaaagc ctttggtctg atcgccgcca
2401 ccggcgatct gccgggggag aggcgtgccc aggcggtcaa cgagctctct accgccgtgg
2461 gcgtgcaggg cctggtactg gggcagtttc gcgatcttaa cgatgccgcc ctcgaccgta
2521 cccctgacgc tatcctcagc accaaccacc tcaagaccgg cattctgttc agcgcgatgc
2581 tgcagatcgt cgccattgct tccgcctcgt cgccgagcac gcgagagacg ctgcacgcct
2641 tcgccctcga cttcggccag gcgtttcaac tgctggacga tctgcgtgac gatcacccgg
2701 aaaccggtaa agatcgcaat aaggacgcgg gaaaatcgac gctggtcaac cggctgggcg
2761 cagacgcggc ccggcaaaag ctgcgcgagc atattgattc cgccgacaaa cacctcactt
2821 ttgcctgtcc gcagggcggc gccatccgac agtttatgca tctgtggttt ggccatcacc
2881 ttgccgactg gtcaccggtc atgaaaatcg cctgataccg cccttttggg ttcaagcagt
2941 acataacgat ggaaccacat tacaggagta gtgatgaatg aaggacgagc gccttgttca
3001 gcgtaagaac gatcatctgg atatcgttct cgacccccgt cgcgccgtaa ctcaggctag
3061 cgcaggtttt gagcgctggc gctttaccca ctgcgccctg ccagagctga attttagcga
3121 catcacgctg gaaaccacct tcctgaatcg gcagctacag gctccgctgc tgatcagctc
3181 catgaccggc ggcgttgagc gctcgcgcca tatcaaccgc cacctcgccg aggcggcgca
3241 ggtgctaaaa attgcgatgg gggtgggctc ccagcgcgtc gccattgaga gcgacgcggg
3301 cttagggctg gataaaaccc tgcggcagct ggctccggac gtgccgctgc tggcgaacct
3361 cggcgcggcg cagctgaccg gcagaaaagg tattgattac gcccgacggg ccgtggagat
3421 gatcgaggcg gatgcgctga ttgtgcacct aaacccgctg caggaggcgc tacagcccgg
3481 cggcgatcgc gactggcgcg gacggctggc ggctattgaa actctggtcc gcgagctgcc
3541 cgttccgctg gtggtgaaag aggtgggagc cggtatctcc cgaaccgtgg ccgggcagct
3601 gatcgatgcc ggcgttaccg tgattgacgt cgcgggcgcg ggcggcacca gctgggccgc
3661 cgttgaaggc gagcgggcgg ccaccgagca gcagcgcagc gtggccaacg tctttgccga
3721 ctgggggatc cccaccgctg aggcgctggt tgacattgcc gaggcctggc cgcagatgcc
3781 ccttattgcc tcgggcggga ttaaaaacgg cgtcgaccag ccgcgctacg ttgccgaggc
3841 taatctggtg atcacccacg gcggtctcaa taccgtactg gatgcgctgg ctgccgcgac
3901 gccggtgctg gcggtgccac tctctttcga ccagcccgcc gtggctgccc ggctggtcta
3961 taacgggctg ggtcgccggg tatcgcgctt tgccagacag cagacgctgg cggatgagat
4021 tgcccaactg ctgggggatg agacgctgca tcagcgtctg gcgacggccc gccagcagct
4081 taacgacgcc gggggcacgc cccgtgcggc gaccctgatt gaacaggcca tagcagggag
4141 tgagagcgta tcgtgaggga tctgatttta gtcggcggcg gcctggccaa cgggctgatc
4201 gcctggcgtc tgcgccagcg ctacccgcag cttaacctgc tgctgatcga ggccggggag
4261 cagcccggcg ggaaccatac ctggtcattc catgaagacg atctgactcc cgggcagcac
4321 gcctggctgg ccccgctggt ggcccacgcc tggccgggct atgaggtgca gtttcccgat
4381 cttcgccgtc gcctcgcgcg cggctactac tccattacct cagagcgctt tgccgaggcc
4441 ctgcatcagg cgctggggga gaacatctgg ctaaactgtt cggtgagcga ggtgttaccc
4501 aatagcgtgc gccttgccaa cggtgaggcg ctgcttgccg gagcggtgat tgacggacgc
4561 ggcgtgaccg ccagttcggc gatgcaaacc ggctatcagc tctttcttgg tcagcagtgg
4621 cggctgacac agccccacgg cctgaccgta ccgatcctga tggatgccac ggtggcgcag
4681 cagcagggct atcgctttgt ctacacgctg ccgctctccg ccgacacgct gctgatcgag
4741 gatacgcgct acgccaatgt cccgcagcgt gatgataatg ccctacgcca gacggttacc
4801 gactatgctc acagcaaagg gtggcagctg gcccagcttg aacgcgagga gaccggctgt
4861 ctgccgatta ccctggcggg tgacatccag gctctgtggg ccgatgcgcc gggcgtgccg
4921 cgctcgggaa tgcgggctgg gctatttcac cctaccactg gctattcgct gccgctggcg
4981 gtggcccttg ccgacgcgat tgccgacagc ccgcggctgg gcagcgttcc gctctatcag
5041 ctcacccggc agtttgccga acgccactgg cgcaggcagg gattcttccg cctgctgaac
5101 cggatgcttt tcctggccgg gcgcgaggag aaccgctggc gggtgatgca gcgcttttat
5161 gggctgccgg agcccaccgt agagcgcttt tacgccggtc ggctctctct ctttgataag
5221 gcccgcattt tgacgggcaa gccaccggtt ccgctgggcg aagcctggcg ggcggcgctg
5281 aaccattttc ctgacagacg agataaagga tgaaaaaaac cgttgtgatt ggcgcaggct
5341 ttggtggcct ggcgctggcg attcgcctgc aggcggcagg gatcccaacc gtactgctgg
5401 agcagcggga caagcccggc ggtcgggcct acgtctggca tgaccagggc tttacctttg
5461 acgccgggcc gacggtgatc accgatccta ccgcgcttga ggcgctgttc accctggccg
5521 gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa acccttctac cgactctgct
5581 gggagtccgg gaagaccctc gactatgcta acgacagcgc cgagcttgag gcgcagatta
5641 cccagttcaa cccccgcgac gtcgagggct accggcgctt tctggcttac tcccaggcgg
5701 tattccagga gggatatttg cgcctcggca gcgtgccgtt cctctctttt cgcgacatgc
5761 tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca gagcgtctac cagtcggttt
5821 cgcgctttat tgaggatgag catctgcggc aggccttctc gttccactcc ctgctggtag
5881 gcggcaaccc cttcaccacc tcgtccatct acaccctgat ccacgccctt gagcgggagt
5941 ggggggtctg gttccctgag ggcggcaccg gggcgctggt gaacggcatg gtgaagctgt
6001 ttaccgatct gggcggggag atcgaactca acgcccgggt cgaagagctg gtggtggccg
6061 ataaccgcgt aagccaggtc cggctggcgg atggtcggat ctttgacacc gacgccgtag
6121 cctcgaacgc tgacgtggtg aacacctata aaaagctgct cggccaccat ccggtggggc
6181 agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa ctcgctgttt gtgctctact
6241 tcggcctgaa ccagcctcat tcccagctgg cgcaccatac catctgtttt ggtccccgct
6301 accgggagct gatcgacgag atctttaccg gcagcgcgct ggcggatgac ttctcgctct
6361 acctgcactc gccctgcgtg accgatccct cgctcgcgcc tcccggctgc gccagcttct
6421 acgtgctggc cccggtgccg catcttggca acgcgccgct ggactgggcg caggaggggc
6481 cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg ctatatgccc ggcctgcgta
6541 gccagctggt gacccagcgg atctttaccc cggcagactt ccacgacacg ctggatgcgc
6601 atctgggatc ggccttctcc atcgagccgc tgctgaccca aagcgcctgg ttccgcccgc
6661 acaaccgcga cagcgacatt gccaacctct acctggtggg cgcaggtact caccctgggg
6721 cgggcattcc tggcgtagtg gcctcggcga aagccaccgc cagcctgatg attgaggatc
6781 tgcaatgagc caaccgccgc tgcttgacca cgccacgcag accatggcca acggctcgaa
6841 aagttttgcc accgctgcga agctgttcga cccggccacc cgccgtagcg tgctgatgct
6901 ctacacctgg tgccgccact gcgatgacgt cattgacgac cagacccacg gcttcgccag
6961 cgaggccgcg gcggaggagg aggccaccca gcgcctggcc cggctgcgca cgctgaccct
7021 ggcggcgttt gaaggggccg agatgcagga tcctctacgc cggacgcatc gtggccggca
7081 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
7141 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
7201 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
7261 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
7321 agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
7381 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
7441 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
7501 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
7561 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
7621 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
7681 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
7741 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
7801 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
7861 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
7921 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
7981 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
8041 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
8101 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
8161 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
8221 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
8281 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcccct acgtgctgct gaagttgccc
8341 gcaacagaga gtggaaccaa ccggtgatac cacgatacta tgactgagag tcaacgccat
8401 gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgtccg gtagctcctt
8461 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
8521 actcgtagga caggtgccgg cagcgcccaa cagtcccccg gccacggggc ctgccaccat
8581 acccacgccg aaacaagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
8641 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctaaccgt ttttatcagg
8701 ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga
8761 gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
8821 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
8881 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
8941 agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
9001 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
9061 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
9121 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
9181 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
9241 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
9301 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
9361 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
9421 acgg
//
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